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| Simultaneous determination by UPLC-MS/MS of seven bioactive compounds in rat plasma after oral administration of Ginkgo biloba tablets: application to a pharmacokinetic study |
| Wen-ping Wang, Na Liu, Qian Kang, Pei-pei Du, Yi Lan, Bo-chen Zhao, Yan-yan Chen, Qing Zhang, Hui Li, Ye-wen Zhang, Qing Wu |
| School of Chinese Material Medica, Beijing University of Chinese Medicine, Beijing 100102, China; Beijing Handian Pharmaceutical Co., Ltd., Beijing 100004, China |
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Abstract A rapid, reliable, and sensitive method was developed using ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) with an electrospray ionization (ESI) source for determination of seven bioactive compounds in rat plasma after oral administration of Ginkgo biloba tablets (GBTs). The method simultaneously detects bilobalide (BB), ginkgolide A (GA), ginkgolide B (GB), ginkgolide C (GC), quercetin (QCT), kaempferol (KMF), and isorhamnetin (ISR) for pharmacokinetic study. The analytes and internal standard (IS) were extracted from rat plasma by acetidin. An MS/MS detection was conducted using multiple reaction monitoring (MRM) and operating in the negative ionization mode. The calibration curve ranges were 5–500, 5–500, 2.5–250, 1–100, 1–100, 1–100, and 1–100 ng/ml for BB, GA, GB, GC, QCT, KMF, and ISR, respectively. The mean recovery of the analytes ranged from 68.11% to 84.42%. The intra- and inter-day precisions were in the range of 2.33%–9.86% and the accuracies were between 87.67% and 108.37%. The method was used successfully in a pharmacokinetic study of GBTs. The pharmacokinetic parameters of seven compounds were analyzed using a non-compartment model. Plasma concentrations of the seven compounds were determined up to 48 h after administration, and their pharmacokinetic parameters were in agreement with previous studies.
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Received: 10 February 2014
Published: 04 November 2014
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Cite this article:
Wen-ping Wang, Na Liu, Qian Kang, Pei-pei Du, Yi Lan, Bo-chen Zhao, Yan-yan Chen, Qing Zhang, Hui Li, Ye-wen Zhang, Qing Wu. Simultaneous determination by UPLC-MS/MS of seven bioactive compounds in rat plasma after oral administration of Ginkgo biloba tablets: application to a pharmacokinetic study. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2014, 15(11): 929-939.
URL:
http://www.zjujournals.com/xueshu/zjus-b/10.1631/jzus.B1400035 OR http://www.zjujournals.com/xueshu/zjus-b/Y2014/V15/I11/929
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