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ZJUS-B
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology)  2007, Vol. 8 Issue (9): 673-679    DOI: 10.1631/jzus.2007.B0673
Biotechnology     
Effect of chlorogenic acid on antioxidant activity of Flos Lonicerae extracts
WU Lan
Department of Horticulture, Beijing Vocational College of Agriculture, Beijing 102442, China
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Abstract  Flos Lonicerae is a medically useful traditional Chinese medicine herb. However, little is known about the antioxidant properties of Flos Lonicerae extracts. Here the antioxidant capacity of water, methanolic and ethanolic extracts prepared from Flos Lonicerae to scavenge 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and reduce Fe3+ to Fe2+ is examined. Chlorogenic acid, a major component of Flos Lonicerae, is identified and further purified from 70% ethanolic extract with high performance liquid chromatography (HPLC) and its antioxidant capacity is characterized. The total phenolic compounds and chlorogenic acid contents in Flos Lonicerae are determined. The present results demonstrate that the Flos Lonicerae extracts exhibit antioxidant activity and chlorogenic acid is a major contributor to this activity.

Key words： Flos Lonicerae      Chlorogenic acid      Antioxidant     
Received: 06 December 2006     
CLC:  R282.71  
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WU Lan. Effect of chlorogenic acid on antioxidant activity of Flos Lonicerae extracts. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2007, 8(9): 673-679.

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http://www.zjujournals.com/xueshu/zjus-b/10.1631/jzus.2007.B0673     OR     http://www.zjujournals.com/xueshu/zjus-b/Y2007/V8/I9/673

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