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Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology)  2014, Vol. 15 Issue (2): 173-180    DOI: 10.1631/jzus.B1300186
Articles     
In vitro antioxidant activity and potential inhibitory action against α-glucosidase of polysaccharides from fruit peel of tea (Camellia sinensis L.)
Yue-fei Wang, Jie Wang, Jing Wu, Ping Xu, Yi-qi Wang, Jun-jie Gao, Danielle Hochstetter
Department of Tea Science, Zhejiang University, Hangzhou 310058, China; Key Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Ministry of Agriculture, Hangzhou 310058, China; College of Pharmacy, Zhejiang Chinese Medical University, Hangzhou 310053, China
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Abstract  The conditions for extracting polysaccharides from tea (Camellia sinensis L.) fruit peel (TFPPs) were studied. Three parameters (temperature, time, and liquid/solid ratio) affecting the extraction of TFPP were optimized using response surface methodology (RSM). Under the optimized conditions, the yield of TFPP was predicted to be 4.98%. The physicochemical properties, in vitro antioxidant activities, and inhibitory effects on α-glucosidase of fractionated TFPPs (TFPP-0, TFPP-20, TFPP-40, and TFPP-60) were investigated. We found that the TFPPs were all acid protein-bound heteropolysaccharides, although with different chemical compositions. They had not only remarkable scavenging activity on 2,2\'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) and reducing activity, but also excellent inhibitory potential against α-glucosidase in vitro. Our results suggest that tea fruit peel could be treated as a potential bioresource for the development of polysaccharide antioxidants.

Key wordsPolysaccharides      Tea (Camellia sinensis L.) fruit peel      Physicochemical properties      Antioxidant activity      Data S1

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Received: 11 July 2013      Published: 28 January 2014
CLC:  S609.9  
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Yue-fei Wang
Ping Xu
Jie Wang
Jing Wu
Yi-qi Wang
Jun-jie Gao
Danielle Hochstetter
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Yue-fei Wang, Jie Wang, Jing Wu, Ping Xu, Yi-qi Wang, Jun-jie Gao, Danielle Hochstetter. In vitro antioxidant activity and potential inhibitory action against α-glucosidase of polysaccharides from fruit peel of tea (Camellia sinensis L.). Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2014, 15(2): 173-180.

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http://www.zjujournals.com/xueshu/zjus-b/10.1631/jzus.B1300186     OR     http://www.zjujournals.com/xueshu/zjus-b/Y2014/V15/I2/173

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