The contradiction between land expansion and ecological protection exists in decisions about urban development. In order to quantitatively identify the key ecological conservation area on a large scale and delineate a rigid urban growth boundary, habitat quality and carbon storage and sequestration modules in the InVEST model were used to quantitatively evaluate the ecosystem services over ten districts, one county-level city, and two counties in Hangzhou. Spatial and data information analyses were used to deal with the win-win areas of habitat quality (as a proxy of biodiversity) and carbon stocks, and the analyses of sensitivity of model parameter and the validity of the results were used to ensure the reliability of the analyses. Results show that spatial differences exist in the levels of habitat quality and carbon storage. Those areas in the southwest have higher levels of ecosystem service than those in the northeast. By integrating ecosystem service evaluation into the urban land use decision-making process, priority conservation areas were defined and references for the delineation of a rigid urban growth boundary were provided.