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浙江大学学报（农业与生命科学版）
浙江大学学报(农业与生命科学版)  2016, Vol. 42 Issue (04): 435-441    DOI: 10.3785/j.issn.1008-9209.2015.09.151
农业科学     
蔗糖和光周期在泸定百合试管鳞茎膨大中的作用机制
周玲云， 高素萍*， 陈锋
四川农业大学风景园林学院，成都 611130
Action mechanism of sucrose and photoperiod on bulblet enlargement of Lilium sargentiae
ZHOU Lingyun, GAO Suping*, CHEN Feng
(School of Landscape Architecture, Sichuan Agricultural University, Chengdu 611130, China)
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摘要： 为研究蔗糖和光周期对泸定百合试管鳞茎膨大的影响及其交互作用，解释两者在鳞茎膨大过程中的作用机制，以泸定百合种球鳞片为外植体诱导形成的无菌丛芽为材料，采用不同质量浓度(30、60、90 g/L)蔗糖与不同光周期(0、8、12、16、24 h/d)的组合处理，测定鳞茎发生和膨大的形态指标及其内源性糖含量与相关酶活性的变化。结果显示：1）在蔗糖质量浓度为60 g/L、光周期为8 h/d组合处理下，鳞茎膨大效果最好，与全黑暗处理间无显著差异。2）在30 g/L蔗糖和各光照时间处理下，蔗糖含量变化趋势为W型，果糖和葡萄糖含量变化趋势为M型；而在60和90 g/L蔗糖处理下，蔗糖含量呈先下降后升高再下降趋势，果糖和葡萄糖含量变化趋势与蔗糖含量变化趋势相反。3）在不同质量浓度蔗糖处理下，蔗糖磷酸合成酶活性的变化趋势与蔗糖含量变化趋势基本一致，而蔗糖合成酶活性的变化趋势与蔗糖含量变化趋势相反。综上，蔗糖质量浓度和光周期对试管小鳞茎的发生和膨大存在交互作用，但蔗糖对百合鳞茎生长与膨大影响更大，是造成鳞茎诱导及膨大的主要因子；蔗糖合成酶参与蔗糖分解代谢过程，而蔗糖磷酸合成酶参与蔗糖积累过程;蔗糖不仅在膨大过程中扮演了生理角色，还可能扮演了糖信号角色，并且这些过程都受到严格的浓度调控.在生产中，推荐的最适组合为蔗糖质量浓度60 g/L，光周期0 h/d。
Abstract:   Lily has high ornamental and economic value. However, the reproduction rate of traditional propagation method was low, and was easily infected, causing degradation of bulbs and associated problems. Application of tissue culture techniques can greatly improve the reproduction coefficient in the production process, but the weakness of plantlets and low survival rate in the transplanting process have become a prominent problem during the commercial process of lily bulbs. Hence, to cultivate strong seedling and increase survival rate, we could promote the formation and enlargement of bulbs by in vitro culture. This study aimed to explore the influence of sucrose and photoperiod on the enlargement of in vitro bulblet of Lilium sargentiae and their interaction effect, and to explain their action mechanism in the process of in vitro bulblet enlargement. Sterile buds induced by bulblet scales of L. sargentiae as explants were selected as materials. The experiments were conducted under three different concentrations of sucrose (30, 60 and 90 g/L) and five different photoperiods (0, 8, 12, 16 and 24 h/d). The morphological index, endogenous sugar and related enzymatic activity changes were measured in formation and enlargement of in vitro bulblet. The results showed that: 1) The optimal condition for bulblet enlargement was sucrose concentration of 60 g/L and photoperiod of 8 h/d. At a certain concentration of sucrose, there was no significant difference between the photoperiods of 8 h/d and 0 h/d. 2) Under the sucrose concentration of 30 g/L and different lighting conditions, the trend was W type for sucrose content, and M type for both fructose and glucose contents; under the sucrose concentrations of 60 and 90 g/L, the trend of sucrose content was fall rise fall, while the trend of fructose and glucose contents was rise fall rise, opposite to that of sucrose content. 3) Among treatments with different sucrose concentrations, the change trend of sucrose phosphate synthase activity was basically the same with that of the sucrose content, but the change trends of the sucrose synthase activity and sucrose content were opposite. In conclusion, 60 g/L sucrose in complete darkness can promote bulblet enlargement in production. The sucrose synthase is involved in decomposition process of sucrose, while the sucrose phosphate synthase is involved in the accumulation process of sucrose. Sucrose not only plays a physiological role, but also may play a sugar signal role in the bulblet enlargement process, both of which are strictly regulated by sugar concentration. Sucrose concentration and photoperiod have interaction effect on the induction and enlargement of in vitro bulblets, but the effect of sucrose is greater. Thus, sucrose is a major factor for the formation and enlargement of in vitro bulblets.
出版日期: 2016-07-20
CLC:  S 682.2  
通讯作者: 高素萍（http://orcid.org/0000-0002-4609-9988），E-mail:gsp65@163.com   
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周玲云
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引用本文:

周玲云，高素萍，陈锋. 蔗糖和光周期在泸定百合试管鳞茎膨大中的作用机制[J]. 浙江大学学报(农业与生命科学版), 2016, 42(04): 435-441.

ZHOU Lingyun, GAO Suping, CHEN Feng. Action mechanism of sucrose and photoperiod on bulblet enlargement of Lilium sargentiae. Journal of Zhejiang University (Agriculture and Life Sciences), 2016, 42(04): 435-441.

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http://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2015.09.151    或    http://www.zjujournals.com/agr/CN/Y2016/V42/I04/435

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