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浙江大学学报（农业与生命科学版）
浙江大学学报(农业与生命科学版)
论文     
牡丹抗逆转录因子基因PsDREB的功能解析
刘慧春*, 马广莹, 朱开元, 邹清成, 周江华, 田丹青
（浙江省农业科学院花卉研究开发中心，杭州 311202）
Functional analysis of stressrelated transcription factor gene PsDREB from Paeonia suffruticosa
LIU Huichun*, MA Guangying, ZHU Kaiyuan, ZOU Qingcheng, ZHOU Jianghua, TIAN Danqing
(Flower Research and Development Center, Zhejiang Academy of Agricultural Sciences, Hangzhou 311202,China)
 全文: PDF(2651 KB)  
摘要： 通过酵母单杂交试验，对牡丹抗逆转录因子基因PsDREB的DNA结合能力及转录激活功能进行分析。结果显示：PsDREB蛋白能够特异性地与顺式作用元件DRE（dehydration responsive element，干旱应答元件）结合，但不能与突变的DRE(mDRE)结合；该蛋白能够激活下游报告基因His的表达，从而使酵母细胞在缺失His的培养基上能够正常生长。说明PsDREB具有转录激活功能。为了进一步研究该转录因子基因PsDREB在植物抗逆反应中的功能，利用农杆菌介导法将其转化到模式植物烟草中，对获得的转基因烟草进行低温、干旱、高盐、脱落酸等胁迫处理。结果发现：与对照相比，超量表达PsDREB基因能够明显提高烟草对逆境胁迫的抗性，尤其是在抗干旱和高盐方面效果显著。
Abstract: Peony (Paeonia suffruticosa) is one of the most famous traditional flowers with profound cultural connotation in China. With the fast development of gardenflower industry, the application of peony increased dramatically for the purpose of landscaping or medicinal uses. However, stress resistance of peony has been poor due to artificial domestication for thousands of years, which greatly restricted the development of peony. With the significant interest of adding new vitality into the flower industry by successfully introducing and cultivating peony in southern China, improving the tolerance of peony cultivars to stress has become crucial. The dehydration responsive element binding protein (DREB) gene is one of the most important transcription factors that was found to be related to adversity resistance. The objective of this study was to analyze the biological function of DREBgene from peony, named as PsDREB, with methods of yeast onehybrid assay and transgenic technology.  In this study, the ability of PsDREB protein binding with DRE and its transcriptional activation activity were tested by yeast onehybrid assay. Results showed that the PsDREB protein was able to combine specifically with the DRE, but unable to combine with the mutated DRE (mDRE), showing a strong transcriptional activity. Subsequently, the PsDREB gene was transferred into tobacco and was mediated by Agrobacterium to determine its biological function in response to adversity. Thereafter, the tolerance ability of the transgenic tobacco was detected under manipulated drought, low temperature, high salt, and abscisic acid (ABA) treatments, as compared to controls. In conclusion, the PsDREB protein has strong binding and transcriptional activities. The overexpression of PsDREBcan obviously enhance plant resistance to abiotic stresses, especially for drought and high salt treatments. These results lay the foundation for further research on the function mode of PsDREB transcription factor, and also supply theoretical and material evidence for excavating more possible stressresistant genes from peony so as to breed out highly stresstolerant transgenic peony.
收稿日期: 2015-05-15 出版日期: 2016-08-31
CLC:  S 682.2  
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刘慧春
马广莹
朱开元
邹清成
周江华
田丹青

引用本文:

刘慧春, 马广莹, 朱开元, 邹清成, 周江华, 田丹青. 牡丹抗逆转录因子基因PsDREB的功能解析[J]. 浙江大学学报(农业与生命科学版), 10.3785/j.issn.1008-9209.2015.05.152.

LIU Huichun, MA Guangying, ZHU Kaiyuan, ZOU Qingcheng, ZHOU Jianghua, TIAN Danqing . Functional analysis of stressrelated transcription factor gene PsDREB from Paeonia suffruticosa. Journal of Zhejiang University (Agriculture and Life Sciences), 10.3785/j.issn.1008-9209.2015.05.152.

链接本文:

http://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2015.05.152    或    http://www.zjujournals.com/agr/CN/Y0/V/I/1

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