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ZJUS-B
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology)  2005, Vol. 6 Issue ( 7): 3-    DOI: 10.1631/jzus.2005.B0622
    
Effects of fructose-1,6-diphosphate on concentration of calcium and activities of sarcoplosnic Ca2+-ATPase in cardiomyocytes of Adriamycin-treated rats
CAI Wei, CHEN Jun-zhu, RUAN Li-ming, WANG Yi-na
Department of Internal Medicine, First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China
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Abstract  Objective: To observe the effects of fructose-1,6-diphosphate (FDP) on serum levels of cardiac troponin I (cTnI) and creatine kinase-MB (CK-MB), as well as the concentration of calcium in cardiomyocytes (Myo[Ca2+]) and activity of sarcoplosnic Ca2+-ATPase (SRCa2+-ATPase) in Adriamycin (ADR)-treated rats. Methods: Rats were intraperitoneally injected with ADR (2.5 mg/kg every other day for 6 times) and then with different dosages of FDP (every other day for twenty-one times). Bi-antibodies sandwich Enzyme linked immune absorption assay (ELISA) was performed to detect serum level of cTnI. CK-MB was detected by monoclonal antibody, Myo[Ca2+] was detected by fluorescent spectrophotometry and the activity of SRCa2+-ATPase was detected by inorganic phosphate method. Results: FDP (300, 600, 1200 mg/kg) significantly reduced the serum levels of cTnI and CK-MB, while at the same time decreased calcium concentration and increased SRCa2+-ATPase activity in cardiomyocytes of ADR-treated rats (P<0.01). Conclusions: FDP might alleviate the cardiotoxic effects induced by ADR through decreasing calcium level as well as increasing SRCa2+-ATPase activity in cardiomyocytes.

Key words： Fructose-1,6-diphosphate      Adriamycin      Cardiomyocyte      Calcium      Sarcoplosnic reticulum Ca2+-ATPase     
Received: 18 October 2004     
CLC:  R331  
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CAI Wei
CHEN Jun-zhu
RUAN Li-ming
WANG Yi-na
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CAI Wei, CHEN Jun-zhu, RUAN Li-ming, WANG Yi-na. Effects of fructose-1,6-diphosphate on concentration of calcium and activities of sarcoplosnic Ca2+-ATPase in cardiomyocytes of Adriamycin-treated rats. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2005, 6( 7): 3-.

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http://www.zjujournals.com/xueshu/zjus-b/10.1631/jzus.2005.B0622     OR     http://www.zjujournals.com/xueshu/zjus-b/Y2005/V6/I 7/3

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