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Influence of casein phosphopeptide-amorphous calcium phosphate application, smear layer removal, and storage time on resin-dentin bonding |
Jun Lin, Wei-ying Zheng, Peng-ruo-feng Liu, Ning Zhang, Hui-ping Lin, Yi-jing Fan, Xin-hua Gu, Oliver Vollrath, Christian Mehl |
Department of Stomatology, the First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China; Institute of Medical Informatics and Statistics, University Hospital Schleswig-Holstein, Campus Kiel, 24105 Kiel, Germany; Department of Prosthodontics, Propaedeutics and Dental Materials, Christian-Albrechts University at Kiel, Arnold-Heller-Stra?e 16, 24105 Kiel, Germany |
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Abstract The aim of this study is to evaluate the influence of Tooth Mousse (TM) application, smear layer removal, and storage time on resin-dentin microtensile bond strength (µTBS). Dentin specimens were divided into two groups: (1) smear layer covered; (2) smear layer removed using 15% EDTA for 90 s. In each group, half the specimens were treated once with TM for 60 min. After bonding procedures using a two-step self-etching adhesive (Clearfil SE Bond (CSE); Kuraray Medical, Tokyo, Japan), an all-in-one adhesive (G-Bond (GB); GC Corp, Tokyo, Japan), and a total-etch adhesive (Adper Single Bond 2 (SB); 3M ESPE, St. Paul, MN, USA), the specimens were stored for 3 d or 6 months in deionized water at 37 °C, and µTBS was tested and analyzed. With the exception of SB (no TM application) and GB, the μTBS was significantly increased for CSE and SB using EDTA pre-conditioning and 3 d of storage (P≤0.001). Bond strength of GB decreased significantly when using EDTA (3 d storage, P<0.05). TM application only increased the μTBS of GB (no EDTA) and SB (with EDTA) after 3 d (P≤0.02). Comparing the adhesives after 3 d of storage, CSE exhibited the greatest μTBS values followed by GB and SB (P≤0.02). The factors of adhesive, EDTA, and TM did not show any significant impact on μTBS when specimens were stored for 6 months (P>0.05). The additional application of TM and EDTA for cavity preparation seems only to have a short-term effect, and no influence on µTBS of dentin bonds after a period of 6 months.
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Received: 14 August 2013
Published: 06 July 2014
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Cite this article:
Jun Lin, Wei-ying Zheng, Peng-ruo-feng Liu, Ning Zhang, Hui-ping Lin, Yi-jing Fan, Xin-hua Gu, Oliver Vollrath, Christian Mehl. Influence of casein phosphopeptide-amorphous calcium phosphate application, smear layer removal, and storage time on resin-dentin bonding. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2014, 15(7): 649-660.
URL:
http://www.zjujournals.com/xueshu/zjus-b/10.1631/jzus.B1300216 OR http://www.zjujournals.com/xueshu/zjus-b/Y2014/V15/I7/649
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