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| Osteoinduction by Ca-P biomaterials implanted into the muscles of mice |
| Rui-na Yang, Feng Ye, Li-jia Cheng, Jin-jing Wang, Xiao-feng Lu, Yu-jun Shi, Hong-song Fan, Xing-dong Zhang, Hong Bu |
| Key Laboratory of Transplant Engineering and Immunology, Ministry of Health, West China Hospital, Sichuan University, Chengdu 610041, China, National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 610065, China, Department of Pathology, West China Hospital, Sichuan University, Chengdu 610041, China |
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Abstract The osteoinduction of porous biphasic calcium phosphate ceramics (BCP) has been widely reported and documented, but little research has been performed on rodent animals, e.g., mice. In this study, we report osteoinduction in a mouse model. Thirty mice were divided into two groups. BCP materials (Sample A) and control ceramics (Sample B) were implanted into the leg muscle, respectively. Five mice in each group were killed at 15, 30, and 45 d after surgery. Sample A and Sample B were harvested and used for hematoxylin and eosin (HE) staining, immunohistochemistry (IHC) staining, and Alizarin Red S staining to check bone formation in the biomaterials. Histological analysis showed that no bone tissue was formed 15 d after implantation (0/5) in either of the two groups. Newly-formed bone tissues were observed in Sample A at 30 d (5/5) and 45 d (5/5) after implantation; the average amounts of newly-formed bone tissues were approximately 5.2% and 8.6%, respectively. However, we did not see any bone tissue in Sample B until 45 d after implantation. Bone-related molecular makers such as bone morphogenesis protein-2 (BMP-2), collagen type I, and osteopontin were detected by IHC staining in Sample A 30 d after implantation. In addition, the newly-formed bone was also confirmed by Alizarin Red S staining. Because this is the report of osteoinduction in the rodent animal on which all the biotechnologies were available, our results may contribute to further mechanism research.
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Received: 04 June 2010
Published: 04 July 2011
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Cite this article:
Rui-na Yang, Feng Ye, Li-jia Cheng, Jin-jing Wang, Xiao-feng Lu, Yu-jun Shi, Hong-song Fan, Xing-dong Zhang, Hong Bu. Osteoinduction by Ca-P biomaterials implanted into the muscles of mice. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2011, 12(7): 582-590.
URL:
http://www.zjujournals.com/xueshu/zjus-b/10.1631/jzus.B1000204 OR http://www.zjujournals.com/xueshu/zjus-b/Y2011/V12/I7/582
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