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浙江大学学报(工学版)
Journal of ZheJiang University (Engineering Science)  2020, Vol. 54 Issue (9): 1690-1696    DOI: 10.3785/j.issn.1008-973X.2020.09.004
    
Experimental study on microbial reinforced calcareous sand using ring shear apparatus
Xuan-chen DING(),Yu-min CHEN*(),Xin-lei ZHANG
Key Laboratory of Ministry of Education for Geomechanics and Embankment Engineering, College of Civil and Transportation Engineering, Hohai University, Nanjing 210098, China
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Abstract  

The calcareous sand reinforced in this experiment was obtained from an island in the South China Sea. The shear strength characteristics of microbially induced carbonate precipitation (MICP) cemented calcareous sand were studied using ring shear tests. The influences of bacterial concentration, immersion time of bacterial solution, cementation solution concentration, treated time, vertical stress and seawater environment were considered. Results show that MICP can effectively improve the shear strength of calcareous sand. When the cementation solution concentration was 0.5 mol/L, the shear strength of the treated samples reached the maximum values, which was about three times of that of the untreated samples, showing a remarkable strain softening phenomenon. Increasing the concentration of the bacteria solution, soaking time, concentration of the reinforcement solution and strengthening time can improve the reinforcement effect, reduce the ratio of residual strength to peak strength, and make the strain softening phenomenon more and more obvious. Although seawater has an inhibitory effect on the reinforcement process of MICP, the application of MICP in this environment can effectively improve the shear strength of calcareous sandy foundation.

Key words： microbial reinforcement      calcareous sand      ring shear tests      shear strength      residual strength     
Received: 04 September 2019      Published: 22 September 2020
CLC:  TU 433  
Corresponding Authors: Yu-min CHEN     E-mail: dingxc2413@163.com;ymchenhhu@163.com
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Xuan-chen DING
Yu-min CHEN
Xin-lei ZHANG
Cite this article:

Xuan-chen DING,Yu-min CHEN,Xin-lei ZHANG. Experimental study on microbial reinforced calcareous sand using ring shear apparatus. Journal of ZheJiang University (Engineering Science), 2020, 54(9): 1690-1696.

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http://www.zjujournals.com/eng/10.3785/j.issn.1008-973X.2020.09.004     OR     http://www.zjujournals.com/eng/Y2020/V54/I9/1690


微生物加固钙质砂环剪试验研究

对取自南海岛礁的钙质砂进行固化处理，通过环剪试验研究微生物诱导碳酸钙沉积（MICP）胶结钙质砂的抗剪强度特性，并考虑菌液浓度、菌液浸泡时间、加固液浓度、加固时间、竖向应力以及海水环境等因素的影响. 结果表明：MICP方法可以有效提高钙质砂的抗剪强度. 当加固液浓度为0.5 mol/L时，加固后试样的抗剪强度达到最大值，约为未加固试样的3倍，并表现出显著的应变软化现象. 提高菌液浓度、菌液浸泡时间、加固液浓度、加固时间均可改善微生物加固效果，减小残余强度与峰值强度的比值，使试样的应变软化现象越来越明显. 海水环境虽对MICP加固过程有抑制作用，但在此环境下采用MICP方法仍能有效提升钙质砂地基的抗剪强度.


关键词： 微生物加固,  钙质砂,  环剪试验,  抗剪强度,  残余强度 
Fig.1 Calcareous sand grain gradation curve
试剂名称 ρ/(g·L?1) 试剂名称 ρ/(g·L?1)
酵母提取物 20 NiCl2·6H2O 0.024
氯化铵 10 蒸馏水 1000
MnCl2·H2O 0.012 ? ?
Tab.1 Microbial culture medium formulation
Fig.2 Calcareous sand sample after microbially induced carbonate precipitation (MICP) treatment
试样环境 σ/kPa OD600 tb / h c / (mol·L?1) t / d
淡水 50 0.380、0.474、0.542、0.633、0.759 2 0.4 3
0.474 1、3、4、5 0.4 3
0.474 2 0.50、0.60、0.75、1.00 3
0.474 2 0.4 4、5、6、7
25、75、100、125 0.474 2 0.4 7
海水 50 0.474 2 0.40、0.50、0.60、0.75、1.00 7
Tab.2 Conditions of sample reinforcement and ring shear test
Fig.3 Shear stress-shear displacement curve of treated calcareous sand samples under different influencing factors
Fig.4 Shear stress-shear displacement curves of treated calcareous sand samples under different vertical stress
Fig.5 Shear stress-shear displacement curves of treated calcareous sand samples in seawater environment
Fig.6 Comparison of peak strength and residual strength of treated calcareous sand samples under different influencing factors
Fig.7 Comparison of peak strength and residual strength of treated calcareous sand samples under different vertical stress conditions
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