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浙江大学学报(工学版)
浙江大学学报(工学版)  2023, Vol. 57 Issue (6): 1100-1110    DOI: 10.3785/j.issn.1008-973X.2023.06.005
土木工程、水利工程     
基于菌促方法和酶促方法的黄河泥沙加固参数试验研究
王钰轲1(),曹天才1,宋迎宾2,*(),邵景干3,余翔1,董博文4
1. 郑州大学 水利科学与工程学院，河南 郑州，450001
2. 黄河水利委员会 黄河水利科学研究院，河南 郑州，450003
3. 河南交院工程技术集团有限公司，河南 郑州，451460
4. 河南省交通规划设计研究院股份有限公司，河南 郑州，451450
Experimental study on solidification parameters of Yellow River silt based on bacteria-induced and enzyme-induced methods
Yu-ke WANG1(),Tian-cai CAO1,Ying-bin SONG2,*(),Jing-gan SHAO3,Xiang YU1,Bo-wen DONG4
1. College of Water Conservancy Engineering, Zhengzhou University, Zhengzhou 450001, China
2. Yellow River Institute of Hydraulic Research, Yellow River Conservancy Commission, Zhengzhou 450003, China
3. Henan Jiaoyuan Engineering Technology Limited Company, Zhengzhou 451460, China
4. Henan Communications Planning and Design Institute Limited Company, Zhengzhou 451450, China
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摘要：

为了改善黄河泥沙的质量和推广其在路基工程中的应用，基于新兴的生物矿化技术，考虑胶结液浓度和灌浆次数，采用无侧限抗压强度（UCS）试验、碳酸钙质量分数测试、扫描电镜（SEM）等方式，对比分析微生物诱导碳酸钙沉积（MICP）与酶诱导碳酸钙沉积（EICP）技术固化黄河泥沙试样的效果. 试验结果表明：大豆粉质量浓度为40 g/L时，大豆脲酶的提取效率最优；培养基溶液pH=8时，细菌和大豆脲酶的活性最好；生物酶和胶结液的体积比为1∶1时，尿素能够与氯化钙充分反应. 增加胶结液浓度、灌浆次数均能够提高黄河泥沙试样的抗压强度，但两者的影响规律不同，胶结液浓度起主导作用. 当胶结液浓度为1 mol/L、灌浆次数为8次时，EICP、MICP技术固化后试样的抗压强度达到最大，分别为954.65、674.98 kPa. 黄河泥沙试样内部生成的碳酸钙和抗压强度呈线性关系，EICP、MICP技术固化后试样的碳酸钙质量分数最大值分别为24.05%、21.35%. 黄河泥沙颗粒表面和颗粒之间的孔隙中均有方解石型碳酸钙附着，但其质量分数在试样内部自上而下逐渐降低. 宏观、微观的角度分析结果均表明，EICP技术相比MICP技术更加适合固化黄河泥沙.
关键词： 黄河泥沙;  微生物诱导碳酸钙沉积(MICP);  酶诱导碳酸钙沉积(EICP);  抗压强度;  碳酸钙    
Abstract:

In order to improve the quality of the Yellow River silt and popularize its application in subgrade engineering, the effects of microbially induced carbonate precipitation (MICP) and enzyme-induced carbonate precipitation (EICP) technology on the solidification of the Yellow River silt were compared and analyzed, based on the emerging biomineralization technology, considering the cement solution concentration and grouting times, using the unconfined compressive strength (UCS) test, mass fraction test of calcium carbonate and scanning electron microscope (SEM). Test results showed that the extraction efficiency of soybean urease was optimal when the mass concentration of soybean flour was 40 g/L. The highest activity of bacteria and soybean urease was obtained when the pH of the medium solution was 8. With a volume ratio of 1∶1 between the biological enzyme and the caking solution, urea reacted sufficiently with calcium chloride. The compressive strength of the Yellow River silt samples can be improved by increasing the cement solution concentration and the grouting times, but the influences of the two are different, and the cement solution concentration plays a leading role. The maximum compressive strength of the specimens cured by the EICP and MICP technology was 954.65 kPa and 674.98 kPa respectively, at the cement solution concentration of 1 mol/L and the grouting times of 8. The calcium carbonate generated in the Yellow River silt sample has a linear relationship with the compressive strength. The maximum mass fraction of calcium carbonate in the sample cured by EICP and MICP technology was 24.05% and 21.35% respectively. Calcite calcium carbonate is attached to the surface of the Yellow River silt particles and the pores between the particles, and the mass fraction of the calcium carbonate gradually decreases from top to bottom in the sample. Analysis of macroscopic and microscopic views indicates that the EICP is more suitable for solidifying the Yellow River silt than the MICP.
Key words: Yellow River silt    microbially induced carbonate precipitation (MICP)    enzyme-induced carbonate precipitation (EICP)    compressive strength    calcium carbonate
收稿日期: 2022-07-16 出版日期: 2023-06-30
CLC:  TU 411  
基金资助: 国家自然科学基金资助项目(52178369，52109140，U2243222)；河南省自然科学基金资助项目 (202300410424)
通讯作者: 宋迎宾     E-mail: ykewang@163.com;2314231473@qq.com
作者简介: 王钰轲（1989—），男，教授，博导，从事土体静动力学特性与本构模拟研究. orcid.org/0000-0003-1849-4857.E-mail： ykewang@163.com
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引用本文:

王钰轲,曹天才,宋迎宾,邵景干,余翔,董博文. 基于菌促方法和酶促方法的黄河泥沙加固参数试验研究[J]. 浙江大学学报(工学版), 2023, 57(6): 1100-1110.

Yu-ke WANG,Tian-cai CAO,Ying-bin SONG,Jing-gan SHAO,Xiang YU,Bo-wen DONG. Experimental study on solidification parameters of Yellow River silt based on bacteria-induced and enzyme-induced methods. Journal of ZheJiang University (Engineering Science), 2023, 57(6): 1100-1110.

链接本文:

https://www.zjujournals.com/eng/CN/10.3785/j.issn.1008-973X.2023.06.005    或    https://www.zjujournals.com/eng/CN/Y2023/V57/I6/1100

图 1  黄河泥沙的颗粒级配曲线
图 2  大豆脲酶溶液
图 3  大豆脲酶活性测试
图 4  活性良好的细菌溶液
图 5  制备好的黄河泥沙试样
图 6  黄河泥沙试样的灌浆过程
试验编号 处理方式 cc/（mol·L?1） Dr/%
N=2 N=4 N=6 N=8
TU-1 TU-2 TU-3 TU-4 EICP 0.25 40
TU-5 TU-6 TU-7 TU-8 0.50
TU-9 TU-10 TU-11 TU-12 0.75
TU-13 TU-14 TU-15 TU-16 1.00
TB-1 TB-2 TB-3 TB-4 MICP 0.25 40
TB-5 TB-6 TB-7 TB-8 0.50
TB-9 TB-10 TB-11 TB-12 0.75
TB-13 TB-14 TB-15 TB-16 1.00
表 1  黄河泥沙固化试验方案
图 7  酸洗和水洗固化后的黄河泥沙
图 8  大豆脲酶的活性变化曲线
图 9  pH对生物酶活性的影响规律
图 10  2种固化技术处理后的黄河泥沙试样
图 11  2种固化技术处理后的试样在不同灌浆次数条件下，抗压强度随胶结液浓度的变化
图 12  2种固化技术处理后的试样在不同胶结液浓度条件下，抗压强度随灌浆次数的变化
图 13  2种固化黄河泥沙试样的抗压强度三维图
图 14  黄河泥沙试样的应力-应变曲线
图 15  黄河泥沙试样的破坏情况
图 16  EICP固化试样的碳酸钙质量分数三维图
图 17  2种固化方式下试样中的碳酸钙质量分数
图 18  EICP固化后试样的碳酸钙质量分数与抗压强度的关系
图 19  黄河泥沙试样SEM图像
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