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浙江大学学报(工学版)
J4  2011, Vol. 45 Issue (8): 1480-1484    DOI: 10.3785/j.issn.1008-973X.2011.08.026
光学工程     
SPR检测器在流动分析系统的应用
陈惠滨1,3, 王晓萍1, 吴宏伟2, 朱岩2, 郦雅平1
1.浙江大学 现代光学仪器国家重点实验室,浙江 杭州 310027；2.浙江大学 理学院化学系,
浙江 杭州 310028；3.集美大学 信息工程学院,福建 厦门 361021
Application of SPR detector for flow analysis system
CHEN Hui-bin1, WANG Xiao-ping1, WU Hong-wei2, ZHU Yan2, LI Ya-ping1
1. State Key Laboratory of Modern Optical Instrumentation, Zhejiang University, Hangzhou 310027, China;
2. Department of Chemistry, College of Science, Zhejiang University, Hangzhou 310028, China；
3. Information Science and Engineering College, Jimei University, Xiamen, 361021, China
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摘要：

为实现通用性强、样品消耗小、成本低新型检测器的开发，设计一种应用于流动分析体系的表面等离子共振（SPR）检测器.以3种糖的标准溶液为样品，开展检测器性能指标测试，得到相对标准偏差（RSD）值在1.3%以下，D木糖、蔗糖、葡萄糖的检测下限分别为92、123、132 ng，在最低检测限至10 000 mg/L的检测范围内，线性拟合相关系数大于0.999.将SPR检测器作为液相色谱系统的检测器，测得分离后标准样品RSD值在2.3%以下，蔗糖和半乳糖的检测下限分别为6、9 μg.结果表明，SPR检测器对于紫外检测或荧光检测不敏感，或者需要通过衍生化才可进行测量的成分，具有优势.
Abstract:

To develop a novel detector with characteristics of versatility, low sample consumption, and inexpensiveness, surface plasma resonance (SPR) detector was designed for flow analysis system. Its performance was evaluated using 3 glucide solutions.. Results showed that the RSD values were below 1.3%, and the detection limits of D-xylose, sucrose and glucose were 92, 123, and 132 ng, respectively. The linearities of three components were excellent between detection limits and 10000 mg/L with correlation coefficients above 0.999. The SPR detector was applied to the liquid chromatography system. The RSD values were below 2.3% for separated samples with standard concentration solutions, it could detect the sucrose and galactose as low as 6 and 9 μg, respectively. It indicates that the detector is preponderant to detect materials insensitive to the ultraviolet detection or fluorescence detection, or which only can be detected by derivatization.
出版日期: 2011-09-08
:  O 439  
基金资助:

国家“863”高科技研究发展计划资助项目 (2007AA09Z114)；浙江省科技计划资助项目（2007C13066）.
通讯作者: 王晓萍，女，教授.     E-mail: xpwang@zju.edu.cn
作者简介: 陈惠滨(1978—)，男，博士生，从事传感器应用研究.E-mail:amoy_chb@126.com
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引用本文:

陈惠滨, 王晓萍, 吴宏伟, 朱岩, 郦雅平. SPR检测器在流动分析系统的应用[J]. J4, 2011, 45(8): 1480-1484.

CHEN Hui-bin, WANG Xiao-ping, WU Hong-wei, ZHU Yan, LI Ya-ping. Application of SPR detector for flow analysis system. J4, 2011, 45(8): 1480-1484.

链接本文:

https://www.zjujournals.com/eng/CN/10.3785/j.issn.1008-973X.2011.08.026    或    https://www.zjujournals.com/eng/CN/Y2011/V45/I8/1480

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