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浙江大学学报（农业与生命科学版）
Journal of Zhejiang University (Agriculture and Life Sciences)  2021, Vol. 47 Issue (6): 683-694    DOI: 10.3785/j.issn.1008-9209.2021.06.151
Reviews     
Research progress on natural products of main horticultural plants in China
Jiaojiao LIANG(),Yunyi CHEN(),Yue WANG,Jiebiao CHEN,Jinping CAO,Xian LI,Chongde SUN()
Laboratory of Fruit Quality Biology/Key Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Ministry of Agriculture and Rural Affairs/Horticultural Products Cold Chain Logistics Technology and Equipment National-Local Joint Engineering Laboratory/Zhejiang Provincial Key Laboratory of Integrative Biology of Horticultural Plants, Zhejiang University, Hangzhou 310058, China
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Abstract  

Natural products are widely found in nature, and their unique chemical diversity endows them with a variety of biological activities related to human health. China is rich in horticultural plant resources, in which the natural products are worthy of in-depth study and utilization. This article mainly focused on the identifi-cation, separation and purification of natural products of main horticultural plants in China and their application in activity evaluation systems, and summarized the progress of related research and the advantages and disadvantages of various evaluation methods, aiming to deepen the utilization of horticultural plant natural products, improve the separation efficiency of active substances and complete the evaluation systems, and further promote the integration and development of horticultural plant natural products related industries in the future.

Key words： horticultural plants      natural products      separation      purification      identification      activity evaluation     
Received: 15 June 2021      Published: 25 December 2021
CLC:  S 6-0  
Corresponding Authors: Chongde SUN     E-mail: 12016038@zju.edu.cn;adesun2006@zju.edu.cn
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Jiaojiao LIANG
Yunyi CHEN
Yue WANG
Jiebiao CHEN
Jinping CAO
Xian LI
Chongde SUN
Cite this article:

Jiaojiao LIANG,Yunyi CHEN,Yue WANG,Jiebiao CHEN,Jinping CAO,Xian LI,Chongde SUN. Research progress on natural products of main horticultural plants in China. Journal of Zhejiang University (Agriculture and Life Sciences), 2021, 47(6): 683-694.

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http://www.zjujournals.com/agr/10.3785/j.issn.1008-9209.2021.06.151     OR     http://www.zjujournals.com/agr/Y2021/V47/I6/683


我国主要园艺植物天然产物研究进展

天然产物在自然界中广泛存在，其独特的化学多样性赋予它多种与人体健康相关的生物活性。我国园艺植物资源丰富，其中所蕴含的天然产物是值得深入研究与利用的资源宝库。本文主要围绕我国主要园艺植物天然产物的鉴定挖掘、分离纯化及其活性评价应用等方面进行综述，总结归纳了相关研究进展以及不同评价方法的优缺点，旨在为我国主要园艺植物天然产物的深度挖掘利用、活性物质分离效率的提高以及评价技术体系的完善提供参考，从而推进未来我国园艺植物天然产物相关产业融合发展的进程。


关键词： 园艺植物,  天然产物,  分离,  纯化,  鉴定,  活性评价 
Fig. 1 Distribution of the representative national horticultural plant germplasm resource nurseries in China
Fig. 2 Main processes and methods for separating and purifying natural products of horticultural plants
Fig. 3 Advantages and disadvantages of different activity evaluation systems
 
[1]   SHEN Y M, HAO X J. Natural product sciences: an integrative approach to the innovations of plant natural products. Science China (Life Sciences), 2020,63(11):1634-1650. DOI:10.1007/s11427-020-1799-y
doi: 10.1007/s11427-020-1799-y
[2]   PHAM J V, YILMA M A, FELIZ A, et al. A review of the microbial production of bioactive natural products and biologics. Frontiers in Microbiology, 2019,10:1404. DOI:10.3389/fmicb.2019.01404
doi: 10
[3]   SOROKINA M, STEINBECK C. Review on natural products databases: where to find data in 2020. Journal of Cheminformatics, 2020,12(9):629-661. DOI:10.1186/s13321-020-00424-9
doi: 10.1186/s13321-020-00424-9
[4]   MARTEL J, OJCIUS D M, KO Y F, et al. Phytochemicals as prebiotics and biological stress inducers. Trends in Biochemical Sciences, 2020,45(6):462-471. DOI:10.1016/j.tibs.2020.02.008
doi: 10.1016/j.tibs.2020.02.008
[5]   ATANASOV A G, ZOTCHEV S B, DIRSCH V M, et al. Natural products in drug discovery: advances and oppor-tunities. Nature Reviews: Drug Discovery, 2021,20(3):200-216. DOI:10.1038/s41573-020-00114-z
doi: 10.1038/s41573-020-00114-z
[6]   NEWMAN D J, CRAGG G M. Natural products as sources of new drugs over the nearly four decades from 01/1981 to 09/2019. Journal of Natural Products, 2020,83(3):770-803. DOI:10.1021/acs.jnatprod.9b01285
doi: 10.1021/acs.jnatprod.9b01285
[7]   PATRIDGE E, GAREISS P, KINCH M S, et al. An analysis of FDA-approved drugs: natural products and their derivatives. Drug Discovery Today, 2016,21(2):204-207. DOI:10.1016/j.drudis.2015.01.009
doi: 10.1016/j.drudis.2015.01.009
[8]   郭瑞霞,李力更,王于方,等.天然药物化学史话:天然产物化学研究的魅力.中草药,2015,46(14):2019-2033. DOI:10.7501/j.issn.0253-2670.2015.14.001
GUO R X, LI L G, WANG Y F, et al. Historical story on natural medicinal chemistry: great charm of study on natural product chemistry. Chinese Traditional and Herbal Drugs, 2015,46(14):2019-2033. (in Chinese with English abstract)
doi: 10.7501/j.issn.0253-2670.2015.14.001
[9]   UGBOGU E A, ELGHANDOUR M, IKPEAZU V O, et al. The potential impacts of dietary plant natural products on the sustainable mitigation of methane emission from livestock farming. Journal of Cleaner Production, 2019,213:915-925. DOI:10.1016/j.jclepro.2018.12.233
doi: 10.1016/j.jclepro.2018.12.233
[10]   LIU R H. Health-promoting components of fruits and vegetables in the diet. Advances in Nutrition, 2013,4(3):384S-392S. DOI:10.3945/an.112.003517
doi: 10.3945/an.112.003517
[11]   武运芳.新疆雪莲植株再生体系的优化及农杆菌介导的遗传转化研究.北京:中央民族大学,2013. DOI:10.5772/22026
WU Y F. Optimization of plant regeneration system and Agrobacterium-mediated genetic transformation of Saussurea involucrata in Xinjiang. Beijing: Minzu University of China, 2013. (in Chinese with English abstract)
doi: 10.5772/22026
[12]   赵红艳,江丽丽,马淼.濒危药用植物天山雪莲高效植株再生体系的建立.种子,2012,31(4):1-3. DOI:10.16590/j.cnki.1001-4705.2012.04.053
ZHAO H Y, JIANG L L, MA M. In vitro micro-propagation system of Saussurea involucrata an endangered Chinese medicinal herb. Seed, 2012,31(4):1-3. (in Chinese with English abstract)
doi: 10.16590/j.cnki.1001-4705.2012.04.053
[13]   靳欣欣,田英姿,英犁,等.新疆地区与黄河流域红富士苹果性状与品质分析.现代食品科技,2016,32(7):249-254. DOI:10.13982/j.mfst.1673-9078.2016.7.038
JIN X X, TIAN Y Z, YING L, et al. Fruit characteristics and quality of Red Fuji apples grown in Xinjiang and Yellow River Basin. Modern Food Science and Technology, 2016,32(7):249-254. (in Chinese with English abstract)
doi: 10.13982/j.mfst.1673-9078.2016.7.038
[14]   严鑫,吴巨友,贡鑫,等.不同产地圆黄梨果实品质差异分析.果树学报,2021. DOI:10.13925/j.cnki.gsxb.20210255
55. DOI:10.13925/j.cnki.gsxb.20210255
doi: 10.13925/j.cnki.gsxb.20210255
[14]   YAN X, WU J Y, GONG X, et al. Analysis on the difference of fruit quality of Wonhwang pear from different regions. Journal of Fruit Science, 2021. (in Chinese with English abstract)
doi: 10.13925/j.cnki.gsxb.20210255
[15]   罗广华,王爱国.植物中的多酚物质对超氧物自由基的清除作用.热带亚热带植物学报,1994,2(4):95-99. DOI:10.3969/j.issn.1005-3395.1994.4.010
LUO G H, WANG A G. Scavenging effect of plant polyphenolics on superoxide radicals. Journal of Tropical and Subtropical Botany, 1994,2(4):95-99. (in Chinese)
doi: 10.3969/j.issn.1005-3395.1994.4.010
[16]   马燕,徐贞贞,邹辉,等.8个品种辣椒籽成分分析与比较.食品科学,2017,38(22):178-183. DOI:10.7506/spkx1002-6630-201722027
MA Y, XU Z Z, ZOU H, et al. Analysis?and?comparison?of?constituents?in?hot?pepper?seeds?of?eight?varieties. Food Science, 2017,38(22):178-183. (in Chinese with English abstract)
doi: 10.7506/spkx1002-6630-201722027
[17]   韩玉竹,李平兰,何琴,等.辣椒籽抗菌肽提取条件优化及分离纯化.食品科学,2019,40(24):258-264. DOI:10.7506/spkx1002-6630-20181112-123
HAN Y Z, LI P L, HE Q, et al. Optimization of extraction conditions, separation and purification of antifungal peptides from hot pepper seeds. Food Science, 2019,40(24):258-264. (in Chinese with English abstract)
doi: 10.7506/spkx
[18]   冯鑫,夏宇,陈贵堂,等.生姜皮多糖的分离纯化及其结构组成分析.食品科学,2017,38(6):185-190. DOI:10.7506/spkx1002-6630-201706029
FENG X, XIA Y, CHEN G T, et al. Purification and structural analysis of polysaccharides from ginger peels. Food Science, 2017,38(6):185-190. (in Chinese with English abstract)
doi: 10.7506/spkx
[19]   王思明,付炎,刘丹,等.天然药物化学史话:“四大光谱”在天然产物结构鉴定中的应用.中草药,2016,47(16):2779-2796. DOI:10.7501/j.issn.0253-2670.2016.16.001
WANG S M, FU Y, LIU D, et al. Historical story on natural medicine chemistry: application of UV, IR, MS, and NMR spectra in structure elucidation of natural products. Chinese Traditional and Herbal Drugs, 2016,47(16):2779-2796. (in Chinese with English abstract)
doi: 10.7501/j.issn.0253-2670.2016.16.001
[20]   郑振佳.牛蒡活性成分与抗Ⅱ型糖尿病功能研究.山东,泰安:山东农业大学,2018. DOI:10.26420/thrombhaemostres.2021.1058
ZHENG Z J. Study on the active components and anti-type Ⅱ diabetes function of Arctium lappa L. (Burdock). Tai’an, Shandong: Shandong Agricultural University, 2018. (in Chinese with English abstract)
doi: 10.26420/thrombhaemostres.2021.1058
[21]   XU L S, ZHANG Y J, WANG L Z. Structure characteristics of a water-soluble polysaccharide purified from dragon fruit (Hylocereus undatus) pulp. Carbohydrate Polymers, 2016,146:224-230. DOI:10.1016/j.carbpol.2016.03.060
doi: 10.1016/j.carbpol.2016.03.060
[22]   王晓天,李兴国,李大龙,等.不同树莓品种中非花色苷酚的鉴定与分析.北方园艺,2021(11):36-43. DOI:10.11937/bfyy.20203753
WANG X T, LI X G, LI D L, et al. Identification and analysis of non-anthocyanin phenolics in various raspberry (Rubus spp.) cultivars. Northern Horticulture, 2021(11):36-43. (in Chinese with English abstract)
doi: 10.11937/bfyy.20203753
[23]   LU S W, ZHANG Y, ZHU K J, et al. The citrus transcription factor CsMADS6 modulates carotenoid metabolism by directly regulating carotenogenic genes. Plant Physiology, 2018,176(4):2657-2676. DOI:10.1104/pp.17.01830
doi: 10.1104/pp.17.01830
[24]   陈学森,王楠,张宗营,等.我国高类黄酮(红皮与红肉)苹果育种取得突破性进展.中国果树,2020(2):6-9. DOI:10.16626/j.cnki.issn1000-047.2020.02.002
CHEN X S, WANG N, ZHANG Z Y, et al. Breakthrough in the breeding of high flavonoid (red-skin and red-fleshed) apple in China. China Fruits, 2020(2):6-9. (in Chinese with English abstract)
doi: 10.16626/j.cnki.issn1000-047.2020.02.002
[25]   吴乾,王艳芳,蔚沐庭,等.红肉苹果果实酚类物质含量及抗氧化性测定.山西农业科学,2020,48(11):1763-1766. DOI:10.3969/j.issn.1002-2481.2020.11.14
WU Q, WANG Y F, YU M T, et al. Determination of phenolics content and antioxidant capacity of red-fleshed apple. Shanxi Agricultural Science, 2020,48(11):1763-1766. (in Chinese with English abstract)
doi: 10.3969/j.issn.1002-2481.2020.11.14
[26]   TAO L, ZHUO Y T, QIAO Z H, et al. Prenylated coumarins from the fruits of Artocarpus heterophyllus with their potential anti-inflammatory and anti-HIV activities. Natural Product Research, 2021. . DOI:10.1080/14786419.2021.1913590
doi: 10.1080/14786419.2021.1913590
[27]   陈士林,孙奕,万会花,等.中药与天然药物2015—2020年研究亮点评述.药学学报,2020,55(12):2751-2776. DOI:10.16438/j.0513-4870.2020-1170
CHEN S L, SUN Y, WAN H H, et al. Highlights on the progress of traditional Chinese medicine and natural drugs during 2015—2020. Acta Pharmaceutica Sinica, 2020,55(12):2751-2776. (in Chinese with English abstract)
doi: 10.16438/j.0513-4870.2020-1170
[28]   ZHANG G J, LI B, CUI H M, et al. Orychophragines A-C, three biologically active alkaloids from Orychophragmus violaceus. Organic Letters, 2018,20:656-659. DOI:10.1021/acs.orglett.7b03801
doi: 10.1021/acs.orglett.7b03801
[29]   ZHAO Q, GAO J J, QIN X J, et al. Hedychins A and B 6, 7-dinorlabdane diterpenoids with a peroxide bridge from Hedychium forrestii. Organic Letters, 2018,20:704-707. DOI:10.1021/acs.orglett.7b03836
doi: 10.1021/acs.orglett.7b03836
[30]   黄永娟,张凤启,杨甜甜,等.EMS诱变甘蓝型油菜获得高油酸突变体.分子植物育种,2011,9(5):611-616. DOI:10.3969/mpb.009.000611
HUANG Y J, ZHANG F Q, YANG T T, et al. High oleate mutants of Brassica napus produced by EMS inducement. Molecular Plant Breeding, 2011,9(5):611-616. (in Chinese with English abstract)
doi: 10.3969/mpb.009.000611
[31]   SONG C, LIU Y F, SONG A P, et al. The Chrysanthemum nankingense genome provides insights into the evolution and diversification of chrysanthemum flowers and medicinal traits. Molecular Plant, 2018,11(12):1482-1491. DOI:10.1016/j.molp.2018.10.003
doi: 10.1016/j.molp.2018.10.003
[32]   SU W B, JING Y, LIN S K, et al. Polyploidy underlies co-option and diversification of biosynthetic triterpene pathways in the apple tribe. PNAS, 2021,118(20):e2101767118. DOI:10.1073/PNAS.2101767118
doi: 10.1073/PNAS.2101767118
[33]   LIU M J, ZHAO J, CAI Q H, et al. The complex jujube genome provides insights into fruit tree biology. Nature Communications, 2014,5:5315. DOI:10.1038/ncomms6315
doi: 10.1038/ncomms6315
[34]   ZHANG Q, WANG L L, LIU Z G, et al. Transcriptome and metabolome profiling unveil the mechanisms of Ziziphus jujuba Mill. peel coloration. Food Chemistry, 2020,312:125903. DOI:10.1016/j.foodchem.2019.125903
doi: 10.1016/j.foodchem.2019.125903
[35]   LIN T, ZHU G T, ZHANG J H, et al. Genomic analyses provide insights into the history of tomato breeding. Nature Genetics, 2014,46:1220-1226. DOI:10.1038/ng.3117
doi: 10.1038/ng.3117
[36]   CHEN W, GAO Y Q, XIE W B, et al. Genome-wide association analyses provide genetic and biochemical insights into natural variation in rice metabolism. Nature Genetics, 2014,46:714-721. DOI:10.1038/ng.3007
doi: 10.1038/ng.3007
[37]   ZHU G T, WANG S C, HUANG Z J, et al. Rewiring of the fruit metabolome in tomato breeding. Cell, 2018,172(1/2):249-261. DOI:10.1016/j.cell.2017.12.019
doi: 10.1016/j.cell.2017.12.019
[38]   GUO S G, ZHAO S J, SUN H H, et al. Resequencing of 414 cultivated and wild watermelon accessions identifies selection for fruit quality traits. Nature Genetics, 2019,51:1616-1623. DOI:10.1038/s41588-019-0518-4
doi: 10.1038/s41588-019-0518-4
[39]   ZHANG M Y, XUE C, HU H J, et al. Genome-wide association studies provide insights into the genetic determi-nation of fruit traits of pear. Nature Communications, 2021,12:1144. DOI:10.1038/S41467-021-21378-Y
doi: 10.1038/S41467-021-21378-Y
[40]   王仁才.果蔬营养与健康.北京:化学工业出版社,2013:184-192.
WANG R C. Nutrition and Health of Fruits and Vegetables. Beijing: Chemical Industry Press, 2013:184-192. (in Chinese)
[41]   刘静波,陈晶晶,王二雷,等.蓝莓果实中花色苷单体的色谱分离纯化.食品科学,2017,38(2):206-213. DOI:10.7506/spkx1002-6630-201702033
LIU J B, CHEN J J, WANG E L, et al. Separation of anthocyanin monomers from blueberry fruits through chroma-tographic techniques. Food Science, 2017,38(2):206-213. (in Chinese with English abstract)
doi: 10.7506/spkx
[42]   PENG J M, LI K K, ZHU W, et al. Separation and purification of four phenolic compounds from persimmon by high-speed counter-current chromatography. Journal of Chromatography B, 2018,1072:78-85. DOI:10.1016/j.jchromb.2017.11.010
doi: 10.1016/j.jchromb.2017.11.010
[43]   MA Q, LUO J G, KONG L Y. Preparative isolation of steroidal saponins from garlic (Allium Sativum L.) using high-speed counter-current chromatography coupled with evaporative light scattering detection. Journal of Liquid Chromatography & Related Technologies, 2011,34(17):1997-2007. DOI:10.1080/10826076.2011.582911
doi: 10.1080/10826076.2011.582911
[44]   LI Y N, YIN L H, ZHENG L L, et al. Application of high-speed counter-current chromatography coupled with a reverse micelle solvent system to separate three proteins from Momordica charantia. Journal of Chromatography B, 2012,895/896:77-82. DOI:10.1016/j.jchromb.2012.03.017
doi: 10.1016/j.jchromb.2012.03.017
[45]   芦鑫,洪梦佳,宋国辉,等.树莓汁中黄酮的初级分离纯化.食品科技,2017,42(2):197-203. DOI:10.13684/j.cnki.spkj.2017.02.039
LU X, HONG M J, SONG G H, et al. Preliminary separation and purification of flavone in raspberry juice. Food Science and Technology, 2017,42(2):197-203. (in Chinese with English abstract)
doi: 10.13684/j.cnki.spkj.2017.02.039
[46]   WANG K B, CHEN Q C, LIN Y, et al. Separation of catechins and O-methylated (－)-epigallocatechin gallate using polyamide thin-layer chromatography. Journal of Chromatography B, 2016,1017/1018:221-225. DOI:10.1016/j.jchromb.2015.11.060
doi: 10.1016/j.jchromb.2015.11.060
[47]   JIN X, LIU M Y, CHEN Z X, et al. Separation and purification of epigallocatechin-3-gallate (EGCG) from green tea using combined macroporous resin and polyamide column chromatography. Journal of Chromatography B, 2015,1002:113-122. DOI:10.1016/j.jchromb.2015.07.055
doi: 10.1016/j.jchromb.2015.07.055
[48]   中华人民共和国卫生部.关于进一步规范保健食品原料管理的通知.2002-02-28.[2021-06-12]. . DOI:10.1145/511446.511450
Ministry of Health of the People’s Republic of China. Notice on further standardizing the management of health food raw materials. 2002-02-28.[].
doi: 10.1145/511446.511450
[49]   高雪婧,罗思奕,汤凯洁,等.山楂粗糖蛋白对高脂小鼠降脂及抗氧化作用的初步研究.食品与发酵工业,2021,47(1):138-142. DOI:10.13995/j.cnki.11-1802/ts.024873
GAO X J, LUO S Y, TANG K J, et al. Effect of crude hawthorn glycoprotein on hypolipidemic and antioxidant activity in high-fat mice. Food and Fermentation Industries, 2021,47(1):138-142. (in Chinese with English abstract)
doi: 10.13995/j.cnki.11-1802/ts.024873
[50]   李丽静,王领弟,吴晓光.山楂叶总黄酮通过调控miR-133b改善缺氧复氧诱导的神经细胞损伤.中成药,2020,42(6):1443-1449. DOI:10.3969/j.issn.1001-1528.2020.06.009
LI L J, WANG L D, WU X G. Effects of hawthorn leaf flavonoids on improvement of hypoxia-reoxygenation-induced neuronal damage via miR-133b regulation. Chinese Traditional Patent Medicine, 2020,42(6):1443-1449. (in Chinese with English abstract)
doi: 10.3969/j.issn.1001-1528.2020.06.009
[51]   FU X T, YANG H H, MA C L, et al. Characterization and inhibitory activities on α-amylase and α-glucosidase of the polysaccharide from blue honeysuckle berries. International Journal of Biological Macromolecules, 2020,163:414-422. DOI:10.1016/j.ijbiomac.2020.06.267
doi: 10.1016/j.ijbiomac.2020.06.267
[52]   PAUL S, ZHANG X M, YANG Y P, et al. Chemical constituents from turnip and their effects on α-glucosidase. Phyton—International Journal of Experimental Botany, 2020,89(1):131-136. DOI:10.32604/phyton.2020.08328
doi: 10.32604/phyton.2020.08328
[53]   ZHONG Y L, XU H, ZHONG Y, et al. Identification and characterization of the Cucurbitacins, a novel class of small-molecule inhibitors of tropomyosin receptor kinase a. BMC Complementary and Alternative Medicine, 2019,19:295. DOI:10.1186/s12906-019-2709-z
doi: 10.1186/s12906-019-2709-z
[54]   CHEN F, HUANG G L, HUANG H L. Preparation, analysis, antioxidant activities in vivo of phosphorylated polysaccharide from Momordica charantia. Carbohydrate Polymers, 2021,252:117179. DOI:10.1016/j.carbpol.2020.117179
doi: 10.1016/j.carbpol.2020.117179
[55]   冯尚坤,陈浩,邵志勇,等.红光处理对萝卜芽菜采后贮藏过程中芥子油苷和抗氧化能力的影响.核农学报,2021,35(6):1340-1346. DOI:10.11869/j.issn.100-8551.2021.06.1340
FENG S K, CHEN H, SHAO Z Y, et al. Effect of red light treatment on glucosinolates and antioxidant capacity in radish sprouts during post-harvest storage. Journal of Nuclear Agricultural Sciences, 2021,35(6):1340-1346. (in Chinese with English abstract)
doi: 10.11869/j.issn.100-8551.2021.06.1340
[56]   赵彤,安慧,李佳文,等.外源茉莉酸对盐芥芥子油苷含量与抗氧化特性的影响.植物生理学报,2021,57(3):614-622. DOI:10.3969/j.issn.1000-3150.2020.12.001
ZHAO T, AN H, LI J W, et al. Effects of exogenous jasmonic acid on glucosinolate content and antioxidant properties of Eutrema salsugineum. Plant Physiology Journal, 2021,57(3):614-622. (in Chinese with English abstract)
doi: 10.3969/j.issn.1000-3150.2020.12.001
[57]   LI Y, CHENG X Y, CHEN C L, et al. Apigenin, a flavonoid constituent derived from P. villosa, inhibits hepatocellular carcinoma cell growth by CyclinD1/CDK4 regulation via p38 MAPK-p21 signaling. Pathology—Research and Practice, 2020,216(1):152701. DOI:10.1016/j.prp.2019.152701
doi: 10.1016/j.prp.2019.152701
[58]   DONG J, ZHANG X, ZHANG L, et al. Quercetin reduces obesity-associated ATM infiltration and inflammation in mice: a mechanism including AMPKα1/SIRT1. Journal of Lipid Research, 2014,55(3):363-374. DOI:10.1194/jlr.M038786
doi: 10.1194/jlr.M038786
[59]   CHEN J B, WANG Y, ZHU T L, et al. Beneficial regulatory effects of polymethoxyflavone-rich fraction from ougan (Citrus reticulata cv. Suavissima) fruit on gut microbiota and identification of its intestinal metabolites in mice. Antioxidants, 2020,9(9):831. DOI:10.3390/antiox9090831
doi: 10.3390/antiox9090831
[60]   JIA C S, CAO D D, JI S P, et al. Whey protein isolate conjugated with xylo-oligosaccharides via Maillard reaction: characterization, antioxidant capacity, and application for lycopene microencapsulation. LWT-Food Science and Tech-nology, 2020,118:108837. DOI:10.1016/j.lwt.2019.108837
doi: 10.1016/j.lwt.2019.108837
[61]   SUN Y, CHI J P, YE X Q, et al. Nanoliposomes as delivery system for anthocyanins: physicochemical characterization, cellular uptake, and antioxidant properties. LWT-Food Science and Technology, 2021,139:110554. DOI: 10.1016/j.lwt.2020.110554
doi: 10.1016/j.lwt.2020.110554
[62]   屠幼英,马驰.近50年我国茶资源综合利用研究成果及应用进展.中国茶叶,2020,42(12):1-8. DOI:10.3969/j.issn.1000-3150.2020.12.001
TU Y Y, MA C. Research achievements and application progress of utilizations of tea resources in China in recent 50 years. China Tea, 2020,42(12):1-8. (in Chinese with English abstract)
doi: 10.3969/j.issn.1000-3150.2020.12.001
[63]   朱纪林,郝建宏.涉及蓝莓的化妆品专利技术综述.江西化工,2019(2):190-192. DOI:10.3969/j.issn.1008-3103.2019.02.055
ZHU J L, HAO J H. Summary of cosmetic patent technology involving blueberries. Jiangxi Chemical Industry, 2019(2):190-192. (in Chinese)
doi: 10.3969/j.issn.1008-3103.2019.02.055
[64]   彭新,牛乐,周宁,等.山药多糖功能活性及新产品开发研究进展.食品研究与开发,2020,41(17):204-209. DOI:10.12161/j.issn.1005-6521.2020.17.033
PENG X, NIU L, ZHOU N, et al. Research progress in functional activity and new product development of Chinese yam polysaccharide. Food Research and Development, 2020,41(17):204-209. (in Chinese with English abstract)
doi: 10.12161/j.issn.1005-6521.2020.17.033
[65]   孙晓波.总皂苷与总黄酮在制备治疗心血管疾病药中的应用:CN201110188718.4. 2011-11-02. DOI:10.1016/j.chmed.2018.12.003
SUN X B. Application of total saponins and total flavonoids in preparing medicines for treating cardiovascular diseases: CN201110188718.4. 2011-11-02. (in Chinese)
doi: 10.1016/j.chmed.2018.12.003
[66]   中国农业科学院农产品加工研究所.一种柑橘乳酸菌饮料及其制备方法:CN201710038172.1. 2017-06-20. DOI:10.1055/s-0037-1604374
Chinese Academy of Agricultural Sciences. Institute of Food Science and Technology. A citrus lactobacillus beverage and preparation method thereof: CN201710038172.1. 2017-06-20. (in Chinese)
doi: 10.1055/s-0037-1604374
[67]   中国农业科学院农产品加工研究所.一种含有多甲氧基黄酮的柑橘风味添加剂及其制备方法:CN201811584224.6. 2019-05-28. DOI:10.1055/s-0039-1688666
Chinese Academy of Agricultural Sciences. Institute of Food Science and Technology. A citrus flavor additive containing polymethoxyflavones and a preparation method thereof: CN201811584224.6. 2019-05-28. (in Chinese)
doi: 10.1055/s-0039-1688666
[68]   中国农业科学院农产品加工研究所.具有改善肠道菌群结构和缓减动脉粥样硬化功能的柑橘产品及其制备方法与应用:CN202010160680.9. 2020-06-05. DOI:10.37544/0720-5953-2020-05-06
Chinese Academy of Agricultural Sciences. Institute of Food Science and Technology. Citrus product with the function of improving the structure of intestinal flora and reducing atherosclerosis and its preparation method and application: CN202010160680.9. 2020-06-05. (in Chinese)
doi: 10.37544/0720-5953-2020-05-06
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