| Biological sciences & biotechnology |
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| Isolation and identification of a red pigment producer endophytic fungus Monascus sanguineus from Rehmannia glutinosa Libosch |
Yatao HE( ),Dandan GAO,Senning GAN,Ting SUN,Kuizheng CAI(),Junlin LIU() |
| 1. College of Life Science and Engineering, Northwest Minzu University, Lanzhou 730124, China |
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Abstract Fungal pigments are the potential resources as a natural food colorant. An endophytic fungus RJL03, which is able to produce abundant soluble red pigments, was isolated from the medicinal plant Rehmannia glutinosa Libosch. For further research and utilization of this strain and its secondary metabolites, morphological and molecular characteristics of RJL03 were identified. For observing the colonial morphology, the strain was cultured on different substrates at 25 ℃ for seven days. The fungus was also observed through light microscope and scanning electron microscope. The colony of this fungus was white at first and turned red at later stage, villous, circular; the mycelium was composed of irregularly branched, septate; conidia had borne terminally on pedicels, single or 2-10 in chain, which was about (8.0-16.5) μm×(7.5-14.0) μm; the cleistothecia was globose, brown, arising terminally from the apex of short hyphae, whose diameter was 32-70 μm; the ascospore was ellipsoidal, smooth, colorless or red, which was about (6.0-7.5) μm×(4.0-5.0) μm. Sequence analysis showed that ITS and 18S of the strain had 100% and 93% similarities with Monascus sanguineus, respectively. In conclusion, morphological results and phylogenetic analysis provided great evidence that endophytic fungus RJL03 was M. sanguineus. This study first reported M. sanguineus isolates from Rehmannia glutinosa Libosch. in China . It is considered that M. sanguineus, unlike M. purpureus, should be an independent species.
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Received: 02 February 2018
Published: 28 March 2019
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Corresponding Authors:
Kuizheng CAI,Junlin LIU
E-mail: heyt1502@163.com;ckz000@126.com;ljl@xbmu.edu.cn
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怀地黄内生产红色素真菌血红红曲霉（Monascus sanguineus）的分离与鉴定
真菌色素是天然食用色素的潜在来源。本文从药用植物怀地黄（Rehmannia glutinosa Libosch.）中分离鉴定出了一株能够产生丰富可溶性红色素的内生红曲霉属真菌RJL03。为进一步研究和利用该菌株及其次级代谢物，对其进行分类鉴定。采用不同的培养基培养并观察RJL03的菌落特征，使用光学显微镜和扫描电子显微镜对菌株形态进行观察发现：该菌株菌落初为白色，后产色素变红，绒毡状，圆形；菌丝分枝，分隔；分生孢子倒梨形，单生或至多10个成短链状，大小为（8.0～16.5） μm×（7.5～14.0） μm；孢子囊球形，外壳褐色，直径32～70 μm；子囊孢子椭圆形，大小为（6.0～7.5） μm×（4.0～5.0） μm,表面平滑，无色或红色。序列分析发现，该菌株ITS和18S rDNA序列与血红红曲霉（Monascus sanguineus）的相似度分别为100%和93%。综合形态学和序列分析的结果，将菌株RJL03鉴定为血红红曲霉。这是首次在我国报道的血红红曲霉怀地黄分离株；另外，通过对比观察认为M. sanguineus是与紫色红曲霉（M. purpureus）不同的独立物种。
关键词：
内生真菌,
红色素,
怀地黄,
红曲霉,
分离鉴定
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