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浙江大学学报（农业与生命科学版）
Journal of Zhejiang University (Agriculture and Life Sciences)  2020, Vol. 46 Issue (1): 126-134    DOI: 10.3785/j.issn.1008-9209.2019.10.111
Research articles     
Isolation, identification and pathogenicity study of pathogens during postharvest storage of sweet cherries
Fengli WANG1,2(),Jiying QIU2,Meixue DAI1,Leilei CHEN2,Shuangzhi ZHAO2,Xue XIN2,Qingxin ZHOU1,2()
1.College of Life Science, Shandong Normal University, Jinan 250014, China
2.Institute of Agro-Food Science and Technology, Shandong Academy of Agricultural Sciences/Key Laboratory of Agro-Products Processing Technology of Shandong Province/Key Laboratory of Novel Food Resources Processing, Ministry of Agriculture and Rural Affairs, Jinan 250100, China
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Abstract  

Six pathogens were isolated from postharvest sweet cherries during the transportation and low-temperature storage. They were Alternaria alternata NCPS1 andNCPS2, Alternaria sp. NCPS3, Penicillium expansum NCPS4, Cladosporium herbarum NCPS5 and Sarocladium terricola NCPS6. Then, the biological characteristics of the six pathogens were studied in three aspects of growth rate, growth temperature and growth pH value. The results showed that the optimum growth temperature of NCPS1, NCPS2, NCPS3, NCPS5 and NCPS6 was 25 ℃, and the optimum pH value was 8. The optimum temperature of NCPS4 was 30 ℃, and it could grow at pH 2-10. Only NCPS1 and NCPS2 could grow at the low temperature of 0 ℃. The results of pathogenicity study showed that the six strains could be divided into strong pathogenic strains of NCPS1, NCPS2, NCPS3, moderate pathogenic strains of NCPS4 and NCPS5, weak pathogenic strains of NCPS6. In a word, it provides a scientific theory basis for the development of low-temperature storage and fresh-keeping technology of the postharvest sweet cherries.

Key words： sweet cherry      pathogen      isolation and identification      biological characteristics      pathogenicity     
Received: 11 October 2019      Published: 25 February 2020
CLC:  TS 255.3  
Corresponding Authors: Qingxin ZHOU     E-mail: 531223831@qq.com;zhouqx0211@163.com
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Fengli WANG
Jiying QIU
Meixue DAI
Leilei CHEN
Shuangzhi ZHAO
Xue XIN
Qingxin ZHOU
Cite this article:

Fengli WANG,Jiying QIU,Meixue DAI,Leilei CHEN,Shuangzhi ZHAO,Xue XIN,Qingxin ZHOU. Isolation, identification and pathogenicity study of pathogens during postharvest storage of sweet cherries. Journal of Zhejiang University (Agriculture and Life Sciences), 2020, 46(1): 126-134.

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http://www.zjujournals.com/agr/10.3785/j.issn.1008-9209.2019.10.111     OR     http://www.zjujournals.com/agr/Y2020/V46/I1/126


甜樱桃采后贮藏环境中病原菌的分离鉴定及其致病力研究

通过对甜樱桃采后储运、低温贮藏过程中腐烂病原菌的分离鉴定，得到6株病原菌，分别为互生链格孢（Alternaria alternata）NCPS1和NCPS2、链格孢（Alternaria sp.）NCPS3、扩展青霉（Penicillium expansum）NCPS4、禾生枝孢（Cladosporium herbarum）NCPS5和土栖帚枝霉（Sarocladium terricola）NCPS6；为了进一步确定环境因子对病原菌生长的影响，从生长速率、生长温度、生长pH值3个方面进行病原菌的生物学特性研究。结果表明，除菌株NCPS4外，其余5株菌的最适生长温度为25 ℃，最适生长pH为8；而菌株NCPS4的最适生长温度为30 ℃，在pH为2～10时均可生长；在0 ℃低温条件下仅NCPS1和NCPS2能正常生长。致病力比较结果表明，NCPS1、NCPS2、NCPS3为强致病力菌株，NCPS4、NCPS5为中等致病力菌株，NCPS6为弱致病力菌株。本研究为甜樱桃采后贮藏及配套保鲜技术的研发提供了重要的理论依据。


关键词： 甜樱桃,  病原菌,  分离鉴定,  生物学特性,  致病力 
Fig. 1 Colony morphology of pathogensA. PDA medium; B. MEA medium; Subscript 1-6: Strains of NCPS1-NCPS6.
Fig. 2 Microstructure of pathogensA. Conidiogenous structure; B. Spore morphology; Subscript 1-6: Strains of NCPS1-NCPS6.
Fig. 3 PCR products detected by agarose gel electrophoresis1-6: Strains of NCPS1-NCPS6; Y: Negative control; M: DL2000 DNA marker.
Fig. 4 Phylogenetic tree for each fungal strain based on ITS rDNA sequence
Fig. 5 Biological characteristics of six pathogensA. Growth rate analysis of pathogens; B. Growth pH value analysis of pathogens; C. Growth temperature analysis of pathogens. Different lowercase letters above the bars indicate significant differences at the 0.05 probability level.

菌株

Strain

平均病斑直径

Average lesion diameter/mm

平均病情指数

Average disease index

致病力

Pathogenicity

NCPS115.0±1.4a100.0±0.0强 Strong
NCPS210.5±2.2b80.0±2.2强 Strong
NCPS39.4±2.0b68.8±8.9强 Strong
NCPS44.8±0.4c37.7±0.4中 Moderate
NCPS54.5±0.4c33.3±0.0中 Moderate
NCPS62.0±0.3d11.1±0.0弱 Weak
Table 1 Pathogenicity of pathogens on postharvest sweet cherry fruits
Fig. 6 Pathogenicity analysis of pathogens on sweet cherry fruitsCK: Control (non-inoculation). n=20.
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