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浙江大学学报（农业与生命科学版）
浙江大学学报（农业与生命科学版）  2022, Vol. 48 Issue (6): 787-796    DOI: 10.3785/j.issn.1008-9209.2022.06.061
研究论文     
风洞条件下高剂量性信息素对二化螟求偶、交配和产卵的影响
郭前爽1(),陈立玲2,隋华3,杨斌3,卓富彦4,朱晓明4,郭荣4,杜永均1()
1.浙江大学农业与生物技术学院农药与环境毒理研究所，杭州 310058
2.吉林省农业技术推广总站，长春 130023
3.吉林省梅河口市农业生态资源环境与农村能源管理站，吉林 通化 135000
4.全国农业技术推广服务中心，北京 100125
Effects of high dose sex pheromone on courtship, mating and oviposition of Chilo suppressalis (Walker) in the wind tunnel
Qianshuang GUO1(),Liling CHEN2,Hua SUI3,Bin YANG3,Fuyan ZHUO4,Xiaoming ZHU4,Rong GUO4,Yongjun DU1()
1.Institute of Pesticide and Environmental Toxicology, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China
2.Jilin Province Agricultural Technology Extension Station, Changchun 130023, China
3.Agricultural Ecological Resource Environment and Rural Energy Management Station of Meihekou City in Jilin Province, Tonghua 135000, Jilin, China
4.National Extension and Service Center of Agricultural Technology, Beijing 100125, China
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摘要：

为了探明在高剂量性信息素环境下二化螟[Chilo suppressalis (Walker)]的求偶、交配和产卵行为，采用风洞试验模拟交配干扰环境，并结合行为观测和化学方法分析性信息素滴度。结果表明：在风洞中，每次释放3.1 mg高剂量Z11-16：Ald或三元混合物[m（Z11-16：Ald）∶m（Z9-16：Ald）∶m（Z13-18：Ald）=10∶1∶1.2]会显著抑制雌蛾的交配率和性信息素滴度，但2个处理间没有显著差异，且雌雄蛾配对数量、喷射间隔时间之间没有显著差异。在喷射或未喷射高剂量Z11-16：Ald环境下，去除触角的雄蛾交配率分别降至（8.3±1.1）%和（10.0±8.9）%。在高剂量性信息素环境下，雌蛾产卵量和卵孵化率没有受到影响；雌蛾日龄显著影响与雄蛾的交配率，1、3、5日龄雌蛾与1日龄雄蛾的交配率分别为（60.0±5.2）%、（31.7±6.0）%、（8.3±3.1）%，且雌蛾随日龄增长产卵量和卵孵化率下降。综上所述，高剂量性信息素环境会抑制二化螟的交配率和性信息素滴度，但不影响雌蛾的产卵量和卵孵化率，而雌蛾日龄对产卵量和卵孵化率有影响；交配率的下降可能与雄蛾嗅觉系统被钝化有关。
关键词： 二化螟;  性信息素;  交配干扰;  交配;  产卵;  日龄    
Abstract:

To explore the courtship, mating and oviposition behavior of Chilo suppressalis (Walker) in a high dose sex pheromone environment, the mating disruption was simulated in a wind tunnel, and the sex pheromone titers were analyzed in combination with behavioral observation and chemical methods. The results showed that in the wind tunnel, the high dose of Z11-16:Ald and the ternary blend [m(Z11-16:Ald)∶ m(Z9-16:Ald)∶m(Z13-18:Ald)=10∶1∶1.2] of 3.1 mg per spraying significantly inhibited the mating rates and sex pheromone titers of female moths, but no significant differences were found between the two treatments. There were no significant differences in the number of mating pairs of male and female moths and the time interval between each spraying. The mating rates of male moths with the removal of antennae decreased to (8.3±1.1)% and (10.0±8.9)%, respectively, in the environment of spraying or not spraying high dose Z11-16:Ald. The number of laid eggs and egg hatching rates were not affected in the high dose sex pheromone environment. The ages of female moths significantly affected the mating rates with male moths. The mating rates of 1, 3 and 5 day-old female moths with 1 day-old male moths were (60.0±5.2)%, (31.7±6.0)%, and (8.3±3.1)%, respectively. The number of laid eggs and egg hatching rates of female moths decreased with the ages. In conclusion, the high dose sex pheromone Z11-16:Ald and complete blend can inhibit the mating rates and sex pheromone titers of C. suppressalis, and have no impact on the number of laid eggs and egg hatching rates of female moths, but the ages of female moths can affect the number of laid eggs and egg hatching rates; and the decrease in mating rates might be related to the inactivation of olfactory system of male moths.
Key words: Chilo suppressalis (Walker)    sex pheromone    mating disruption    mating    oviposition    age
收稿日期: 2022-06-06 出版日期: 2022-12-27
CLC:  S 435.112.1  
基金资助: 浙江省重点研发计划项目(2018C02027)
通讯作者: 杜永均     E-mail: qianshuang@zju.edu.cn;yongjundu@zju.edu.cn
作者简介: 郭前爽（https://orcid.org/0000-0003-0645-4030），E-mail：qianshuang@zju.edu.cn
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引用本文:

郭前爽,陈立玲,隋华,杨斌,卓富彦,朱晓明,郭荣,杜永均. 风洞条件下高剂量性信息素对二化螟求偶、交配和产卵的影响[J]. 浙江大学学报（农业与生命科学版）, 2022, 48(6): 787-796.

Qianshuang GUO,Liling CHEN,Hua SUI,Bin YANG,Fuyan ZHUO,Xiaoming ZHU,Rong GUO,Yongjun DU. Effects of high dose sex pheromone on courtship, mating and oviposition of Chilo suppressalis (Walker) in the wind tunnel. Journal of Zhejiang University (Agriculture and Life Sciences), 2022, 48(6): 787-796.

链接本文:

https://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2022.06.061    或    https://www.zjujournals.com/agr/CN/Y2022/V48/I6/787

图1  高剂量性信息素环境下的雌蛾交配率A.释放组分为Z11-16：Ald；B.释放组分为m（Z11-16：Ald）∶m（Z9-16：Ald）∶m（Z13-18：Ald）=10∶1∶1.2。CK：乙醇（下同）。短栅上不同小写字母表示相同配对数量的雌蛾交配率在P＜0.05水平差异有统计学意义（LSD法）。
图2  高剂量性信息素环境下雄蛾的交配次数（A）和移去触角后的交配率（B）*表示在P＜0.05水平差异有统计学意义，ns表示在P＜0.05水平差异无统计学意义（学生t检验）。图3和图6同。
图3  高剂量性信息素处理对雌蛾产卵量（A）和卵孵化率（B）的影响
图4  高剂量性信息素处理对羽化后不同日龄雌蛾日产卵量的影响相同日龄的处理组和对照组雌蛾产卵量在P＜0.05水平差异无统计学意义（学生t检验）。
图5  高剂量性信息素环境下释放性信息素的雌蛾百分比*表示在P＜0.05水平差异有统计学意义（卡方检验）。
图6  高剂量性信息素环境下雌蛾释放的性信息素滴度（A）和分泌到腺体外的性信息素量（B）
图7  雌蛾日龄与交配率之间的关系短栅上不同小写字母表示在P＜0.05水平差异有统计学意义（LSD法），下同。
图8  雌蛾日龄对产卵量和卵孵化率的影响
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