西藏濒危植物巨柏根际细菌群落特征

doi:10.3785/j.issn.1008-9209.2022.03.071

浙江大学学报（农业与生命科学版）

2023, Vol. 49

Issue (2): 241-252 DOI: 10.3785/j.issn.1008-9209.2022.03.071

资源利用与环境保护

西藏濒危植物巨柏根际细菌群落特征

巩文峰¹(

),王泽莹²,刘金良³,孙玉¹,杨欣欣²,魏帅²,魏丽萍²(

)

^1.西藏农牧学院植物科学学院, 西藏林芝 860000
^2.西藏农牧学院资源与环境学院, 西藏林芝 860000
^3.西北农林科技大学林学院, 陕西杨凌 712100

Characteristics of the rhizosphere bacterial community of endangered plant Cupressus gigantea in Tibet

Wenfeng GONG¹(

),Zeying WANG²,Jinliang LIU³,Yu SUN¹,Xinxin YANG²,Shuai WEI²,Liping WEI²(

)

^1.College of Plant Sciences, Tibet Agricultural and Animal Husbandry University, Linzhi 860000, Xizang, China
^2.College of Resources and Environment, Tibet Agricultural and Animal Husbandry University, Linzhi 860000, Xizang, China
^3.College of Forestry, Northwest A & F University, Yangling 712100, Shaanxi, China

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摘要：

根际微生物在植物生长和环境适应中起着重要的作用。为揭示西藏濒危珍稀植物巨柏根际细菌群落特征，通过16S rRNA基因扩增子高通量测序技术，并结合土壤化学特性，分析西藏林芝市巴宜区、米林县、朗县3个种群巨柏根际细菌的组成、多样性及影响因子。结果显示：巨柏根际细菌多样性丰富，其中优势菌门为放线菌门（Actinobacteriota）、变形菌门（Proteobacteria）和酸杆菌门（Acidobacteriota）。朗县、巴宜区和米林县巨柏根际细菌共有属为757个（65.83%），独有属分别为125个（10.87%）、39个（3.39%）和41个（3.56%）；基于Bray-Curtis距离的非度量多维尺度的β多样性分析显示，不同种群间根际细菌群落差异显著。细菌群落的共现性网络正相关连接均在68%以上，表明群落间合作关系大于竞争关系。土壤中有效磷、全磷、铵态氮是驱动根际细菌群落结构变化的重要因子。为适应不同生长环境，各种群巨柏形成了特有的根际细菌群落。本研究结果可为探究根际微生物在巨柏生长和环境适应中的作用及保护巨柏植物资源提供参考。

关键词： 巨柏; 根际细菌; 群落结构; 土壤化学特性

Abstract:

Rhizosphere microorganisms play an important role in plant growth and adaptation to the environment. In order to reveal the characteristics of rhizosphere bacterial community of endangered plant Cupressus gigantea in Tibet, we employed 16S rRNA gene high-throughput amplicon sequencing technology combined with soil chemical properties to examine the rhizosphere bacterial composition and diversity in Bayi District, Milin County, and Langxian County of Nyingchi City in Tibet and their influencing factors. The results showed that the diversity of bacteria in the rhizosphere of C. gigantea was rich, and the dominant bacterial phylum were Actinobacteriota, Proteobacteria, and Acidobacteriota. There were 757 shared genera (65.83%) of rhizosphere bacteria in C. gigantea in Langxian County, Bayi District, and Milin County, with 125 (10.87%), 39 (3.39%) and 41 (3.56%) unique genera, respectively, and the β-diversity analysis of non-metric multidimensional scaling based on Bray-Curtis distance revealed significant differences among populations. The positive correlation of the co-occurrence network of bacterial communities was more than 68%, and the cooperative relationship was greater than the competitive relationship. Soil available phosphorus (AP), total phosphorus (TP), and ammonium nitrogen (NH₄⁺-N) were important factors driving changes in the structure of rhizosphere bacterial community. In order to adapt to different growth environments, various groups of C. gigantea have formed unique rhizosphere bacterial communities. The above results can provide a reference for studying the role of rhizosphere microorganisms in the growth and environmental adaptation of C. gigantea and the protection of C. gigantea resources.

Key words: Cupressus gigantea rhizosphere bacteria community structure soil chemical characteristics

收稿日期: 2022-03-07 出版日期: 2023-04-25

CLC:

Q938.1

基金资助: 西藏农牧学院林学学科创新团队建设项目(藏财预指2020-001);2022年度西北农林科技大学-西藏农牧学院科研联合基金项目(XNLH2022-02);西藏高原生态安全联合重点实验室研究生开放基金项目(STAQ-2021Y-11)

通讯作者: 魏丽萍 E-mail: zkxygwf@xza.edu.cn;34984262@qq.com

作者简介: 巩文峰（https://orcid.org/0000-0002-3168-5052），E-mail：zkxygwf@xza.edu.cn

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引用本文:

巩文峰,王泽莹,刘金良,孙玉,杨欣欣,魏帅,魏丽萍. 西藏濒危植物巨柏根际细菌群落特征[J]. 浙江大学学报（农业与生命科学版）, 2023, 49(2): 241-252.

Wenfeng GONG,Zeying WANG,Jinliang LIU,Yu SUN,Xinxin YANG,Shuai WEI,Liping WEI. Characteristics of the rhizosphere bacterial community of endangered plant Cupressus gigantea in Tibet. Journal of Zhejiang University (Agriculture and Life Sciences), 2023, 49(2): 241-252.

链接本文:

https://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2022.03.071 或 https://www.zjujournals.com/agr/CN/Y2023/V49/I2/241

图1 3个种群巨柏根际细菌组成及差异分析*表示在P＜0.05水平差异有统计学意义。

采样点 Sampling site	编号 Serial number	纬度 Latitude (N)	经度 Longitude (E)	海拔 Altitude/m	胸径 Diameter at breast height/m	生境 Habitat	主要伴生种 Main associated species
巴宜区 Bayi District (BY)	1	29°37 $′$ 36.94 $″$	94°23 $′$ 57.73 $″$	3 105.00	0.12	高山峡谷半阳坡沙质土疏林地	绢毛蔷薇（Rosa sericea）、二色锦鸡儿（Caragana bicolor）、楔叶绣线菊（Spiraea canescens）等
	2	29°37 $′$ 36.22 $″$	94°23 $′$ 55.06 $″$	3 064.00	0.15
	3	29°37 $′$ 32.64 $″$	94°23 $′$ 59.75 $″$	3 068.00	0.24
	4	29°37 $′$ 30.69 $″$	94°24 $′$ 04.34 $″$	3 068.00	0.77
	5	29°37 $′$ 38.54 $″$	94°23 $′$ 56.18 $″$	3 128.00	1.27
	6	29°37 $′$ 23.66 $″$	94°24 $′$ 07.46 $″$	3 063.00	1.50
	7	29°37 $′$ 29.88 $″$	94°23 $′$ 29.19 $″$	3 003.00	1.78
	8	29°37 $′$ 38.51 $″$	94°23 $′$ 57.58 $″$	3 126.00	1.87
	9	29°37 $′$ 37.77 $″$	94°23 $′$ 57.51 $″$	3 116.00	2.44
朗县 Langxian County (LX)	1	28°59 $′$ 35.57 $″$	93°18 $′$ 23.40 $″$	3 030.00	0.10	高山峡谷阳坡沙质土疏林地	砂生槐（Sophora moorcroftiana）、西藏野丁香（Leptodermis xizangensis）、猪毛蒿（Artemisia scoparia）、木帚栒子（Cotoneaster dielsianus）、米林蔓黄芪（Phyllolobium milingense）、翅果蓼（Fagopyrum tibeticum）等
	2	28°59 $′$ 09.00 $″$	93°14 $′$ 00.67 $″$	3 002.00	0.13
	3	29°04 $′$ 03.84 $″$	93°04 $′$ 37.60 $″$	3 088.00	0.50
	4	29°04 $′$ 03.88 $″$	93°04 $′$ 36.50 $″$	3 068.00	0.57
	5	29°04 $′$ 02.69 $″$	93°04 $′$ 38.76 $″$	3 100.00	0.72
	6	28°59 $′$ 32.66 $″$	93°18 $′$ 23.12 $″$	3 076.00	0.98
	7	29°04 $′$ 02.37 $″$	93°04 $′$ 38.39 $″$	3 100.00	1.61
	8	29°00 $′$ 47.70 $″$	93°19 $′$ 00.88 $″$	3 014.00	2.25
	9	29°04 $′$ 19.88 $″$	93°23 $′$ 11.80 $″$	3 030.00	3.00
米林县 Milin County (ML)	1	29°07 $′$ 39.90 $″$	93°48 $′$ 49.27 $″$	3 063.00	0.20	高山峡谷阳坡沙质土疏林地	高山松（Pinus densata）、砂生槐（Sophora moorcroftiana）、西南野丁香（Leptodermis purdomii）、烦果小檗（Berberis ignorata ）、二色锦鸡儿（Caragana bicolor）、川藏香茶菜（Isodon pharicus）等
	2	29°05 $′$ 24.80 $″$	93°23 $′$ 59.60 $″$	3 115.00	0.37
	3	29°09 $′$ 04.36 $″$	93°27 $′$ 55.24 $″$	2 982.00	0.45
	4	29°08 $′$ 39.80 $″$	93°27 $′$ 45.00 $″$	3 057.00	0.87
	5	29°05 $′$ 24.80 $″$	93°23 $′$ 59.60 $″$	3 115.00	1.03
	6	29°08 $′$ 39.80 $″$	93°27 $′$ 45.00 $″$	3 057.00	1.38
	7	29°08 $′$ 39.80 $″$	93°27 $′$ 45.00 $″$	3 057.00	1.67
	8	29°07 $′$ 33.22 $″$	93°51 $′$ 11.80 $″$	3 046.00	2.06
	9	29°07 $′$ 32.79 $″$	93°51 $′$ 11.50 $″$	3 050.00	2.29

表1 采样点基本信息

表2 3个种群巨柏根际土壤化学特征

图2 巨柏根际细菌属水平的维恩图

表3 巨柏根际细菌菌群 α 多样性分析

图3 基于Bray-Curtis距离的非度量多维尺度的 β 多样性分析Adonis：应力值 Stress value=0.15；R2=0.12；p=0.01。

图4 3个种群巨柏根际细菌网络分析A. 3个种群巨柏根际细菌网络图；B. 3个种群巨柏根际细菌节点在模块内（Zi）和模块间（Pi）的连通性。

表4 巨柏根际细菌群落网络特征

图5 土壤化学因子与巨柏根际细菌门的皮尔逊相关性分析*表示在P＜0.05水平显著相关；**表示在P＜0.01水平极显著相关。n=27。

图6 土壤化学因子与巨柏根际细菌OTUs的冗余分析图中红色点表示样本组；黑色箭头表示OTUs，红色箭头表示数量型土壤化学因子，箭头长短代表各因子对根际细菌群落的影响程度（解释量）。

表5 土壤化学因子冗余分析

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