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浙江大学学报(农业与生命科学版)  2023, Vol. 49 Issue (1): 65-75    DOI: 10.3785/j.issn.1008-9209.2021.12.281
植物保护     
内蒙古阴山沿麓马铃薯枯萎病病原菌的分离鉴定及其生物学特性
贾瑞芳1,2(),康立茹2,赵远征1,安乐3,张之为2,赵君2,徐利敏1(),张键2()
1.内蒙古自治区农牧业科学院植物保护研究所,内蒙古 呼和浩特 010031
2.内蒙古农业大学园艺与植物保护学院,内蒙古 呼和浩特 010018
3.凉城县农牧和科技局农广校,内蒙古 乌兰察布 013750
Isolation, identification and their biological characteristics of pathogens of potato Fusarium wilt in Yinshan area, Inner Mongolia
Ruifang JIA1,2(),Liru KANG2,Yuanzheng ZHAO1,Le AN3,Zhiwei ZHANG2,Jun ZHAO2,Limin XU1(),Jian ZHANG2()
1.Plant Protection Institute, Inner Mongolia Academy of Agricultural & Animal Husbandry Sciences, Huhhot 010031, Inner Mongolia, China
2.College of Horticulture and Plant Protection, Inner Mongolia Agricultural University, Huhhot 010018, Inner Mongolia, China
3.Agricultural Broadcasting School, Liangcheng County Agricultural and Animal Husbandry and Technology Bureau, Ulanqab 013750, Inner Mongolia, China
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摘要:

为明确内蒙古阴山沿麓地区马铃薯枯萎病病原菌种类及其生物学特性,本研究以2018—2019年采集的463份马铃薯枯萎病植株为材料,采用组织分离法对病原菌进行分离纯化,按照柯赫氏法则进行致病力检测,利用形态学特征结合延伸因子1α(elongation factor 1α, EF1α)测序结果明确病原菌种类及分类地位,并对其生物学特性进行比较研究。结果显示:分离得到的139株镰刀菌属真菌均能引起马铃薯枯萎病,这些镰刀菌分属于10个种,分别为尖孢镰刀菌(Fusarium oxysporum)64株、木贼镰刀菌(F. equiseti)30株、轮枝镰刀菌(F. verticilloides)10株、锐顶镰刀菌(F. acuminatum )12株、芬芳镰刀菌(F. redolens)4株、茄病镰刀菌(F. solani)6株、三线镰刀菌(F. tricinctum)7株、层出镰刀菌(F. proliferum)2株、接骨木镰刀菌(F. sambucinum)3株、厚垣镰刀菌(F. chlamydosporum)1株,其中尖孢镰刀菌的分离率为46.0%,为优势分离菌株。生物学特性测定结果表明:供试镰刀菌在马铃薯葡萄糖琼脂培养基平板上的适宜生长pH为7~9,锐顶镰刀菌、接骨木镰刀菌的最适生长温度为25 ℃,其余镰刀菌种最适生长温度为30 ℃。通过致病力测定发现:鉴定出的10种镰刀菌的致病力差异显著,其中尖孢镰刀菌是引起内蒙古阴山沿麓地区马铃薯枯萎病的优势病原菌,发病植株病情指数为60.19,显著高于其他9种镰刀菌。

关键词: 马铃薯枯萎病镰刀菌生物学特性致病力    
Abstract:

To clarify the pathogen species and their biological characteristics of potato Fusarium wilt in Yinshan area of Inner Mongolia, 463 samples infected with potato wilt were selected as objects from 2018 to 2019. Pathogens were isolated and purified by tissue isolation and the pathogenicity was detected by Koch’s postulate. The types and classification of pathogens were identified by morphology and elongation factor 1α (EF1α) sequencing analysis, and the comparative study of their biological characteristics was conducted. The results showed that 139 strains of Fusarium spp. caused potato Fusarium wilt, which belonging to 10 species, among them, 64 strains of F. oxysporum, 30 strains of F. equiseti, 10 strains of F. verticillioides, 12 strains of F. acuminatum, four strains of F. redolens, six strains of F. solani, seven strains of F. tricinctum, two strains of F. proliferum, three strains of F. sambucinum and one strain of F. chlamydosporum were identified,and theisolation rate of F. oxysporum was 46.0% and it was the dominant isolate. The results of biological characteristic determination showed that the optimum growth pH value of all tested strains on potato dextrose agar (PDA) was 7-9. The optimum growth temperatures of F. acuminatum and F. sambucinum were both 25 ℃, and the other Fusarium species were 30 ℃. Further analysis of pathogenicity showed that there were differences among ten Fusarium wilt pathogens. The disease index caused by F. oxysporum was 60.19, and it was higher than other nine Fusarium species, which was the dominant pathogen of potato Fusarium wilt in Yinshan area of Inner Mongolia.

Key words: potato Fusarium wilt    Fusarium spp.    biological characteristics    pathogenicity
收稿日期: 2021-12-28 出版日期: 2023-02-25
CLC:  S432.1  
基金资助: 内蒙古自治区科技计划项目(2021GG0010);内蒙古农牧业创新基金项目(2019CXJJN07-2)
通讯作者: 徐利敏,张键     E-mail: jiaruifang1028@emails.imau.edu.cn;xulimin@163.com;zhj19890128@126.com
作者简介: 贾瑞芳(https://orcid.org/0000-0001-6761-2085),E-mail:jiaruifang1028@emails.imau.edu.cn
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引用本文:

贾瑞芳,康立茹,赵远征,安乐,张之为,赵君,徐利敏,张键. 内蒙古阴山沿麓马铃薯枯萎病病原菌的分离鉴定及其生物学特性[J]. 浙江大学学报(农业与生命科学版), 2023, 49(1): 65-75.

Ruifang JIA,Liru KANG,Yuanzheng ZHAO,Le AN,Zhiwei ZHANG,Jun ZHAO,Limin XU,Jian ZHANG. Isolation, identification and their biological characteristics of pathogens of potato Fusarium wilt in Yinshan area, Inner Mongolia. Journal of Zhejiang University (Agriculture and Life Sciences), 2023, 49(1): 65-75.

链接本文:

https://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2021.12.281        https://www.zjujournals.com/agr/CN/Y2023/V49/I1/65

图1  马铃薯枯萎病菌10个不同菌株的菌落及分生孢子形态A.对应菌株菌落的正面图;B.对应菌株菌落的背面图;C.对应菌株的分生孢子形态。
图2  马铃薯枯萎病分离株的致病力测定A.对照植株;B.灌根接菌植株;C~E.分离菌株的菌落和分生孢子形态;F~H.病健交界处再分离菌株的菌落和分生孢子形态。
图3  基于 EF1α 基因序列采用邻接法构建马铃薯枯萎病菌的系统发育树
图4  马铃薯幼苗接种不同种的镰刀菌属菌株28 d后的症状
1 徐进,朱杰华,杨艳丽,等.中国马铃薯病虫害发生情况与农药使用现状[J].中国农业科学,2019,52(16):2800-2808. DOI:10.3864/j.issn.0578-1752.2019.16.006
XU J, ZHU J H, YANG Y L, et al. Status of major diseases and insect pests of potato and pesticide usage in China[J]. Scientia Agricultura Sinica, 2019, 52(16): 2800-2808. (in Chinese with English abstract)
doi: 10.3864/j.issn.0578-1752.2019.16.006
2 OMMATI F, ZAKER M, MOHAMMADI A. Biological control of Fusarium wilt of potato (Fusarium oxysporum f. sp. tuberosi) by Trichoderma isolates under field condition and their effect on yield[J]. Journal of Crop Protection, 2013, 2(4): 435-442.
3 安小敏,胡俊,武建华,等.马铃薯枯萎病病原菌研究概述[J].中国马铃薯,2017,31(5):302-306. DOI:10.3969/j.issn.1672-3635.2017.05.010
AN X M, HU J, WU J H, et al. Overview of pathogen causing potato Fusarium wilt[J]. Chinese Potato Journal, 2017, 31(5): 302-306. (in Chinese with English abstract)
doi: 10.3969/j.issn.1672-3635.2017.05.010
4 MEJDOUB-TRABELSI B, JABNOUN-KHIAREDDINE H, ABDALLAH R A B, et al. Effect of single and combined inoculations of potato plants with four fusarium species on wilt severity, plant growth, and production[J]. International Journal of Phytopathology, 2020, 9(2): 123-137. DOI: 10.33687/phytopath.009.02.3344
doi: 10.33687/phytopath.009.02.3344
5 罗爱花,陆立银,胡新元,等.高寒阴湿旱作区马铃薯主粮化品种绿色高效栽培技术研究[J].中国马铃薯,2019,33(4):211-216. DOI:10.3969/j.issn.1672-3635.2019.04.003
LUO A H, LU L Y, HU X Y, et al. Studies on green and high-efficiency cultivation techniques of rain-fed staple food potato in mountainous region of cool and humidity[J]. Chinese Potato Journal, 2019, 33(4): 211-216. (in Chinese with English abstract)
doi: 10.3969/j.issn.1672-3635.2019.04.003
6 耿妍,韩翠仙,张爱香,等.马铃薯枯萎病的药剂筛选及室内毒力测定[J].河北北方学院学报(自然科学版),2019,35(11):36-39. DOI:10.3969/j.issn.1673-1492.2019.11.008
GENG Y, HAN C X, ZHANG A X, et al. Screening of medicaments for potato Fusarium wilt and determination of indoor toxicity[J]. Journal of Hebei North University (Natural Science Edition), 2019, 35(11): 36-39. (in Chinese with English abstract)
doi: 10.3969/j.issn.1673-1492.2019.11.008
7 黄冲,刘万才.近年我国马铃薯病虫害发生特点与监控对策[J].中国植保导刊,2016,36(6):48-52. DOI:10.3969/j.issn.1672-6820.2016.06.011
HUANG C, LIU W C. Occurrence characteristics and monitoring countermeasures of potato diseases and pests in China in recent years[J]. China Plant Protection, 2016, 36(6): 48-52. (in Chinese)
doi: 10.3969/j.issn.1672-6820.2016.06.011
8 陈慧,薛玉凤,蒙美莲,等.内蒙古马铃薯枯萎病病原菌鉴定及其生物学特性[J].中国马铃薯,2016,30(4):226-234. DOI:10.3969/j.issn.1672-3635.2016.04.007
CHEN H, XUE Y F, MENG M L, et al. Pathogen identification and biological characteristics of potato wilt in Inner Mongolia[J]. Chinese Potato Journal, 2016, 30(4): 226-234. (in Chinese with English abstract)
doi: 10.3969/j.issn.1672-3635.2016.04.007
9 王喜刚,杨波,郭成瑾,等.宁夏回族自治区马铃薯镰刀菌根腐病病原菌的分离鉴定与致病性测定[J].植物保护学报,2020,47(3):609-619. DOI:10.13802/j.cnki.zwbhxb.2020.2019073
WANG X G, YANG B, GUO C J, et al. Identification and pathogenicity test of pathogens causing potato Fusarium root rot in Ningxia Hui Autonomous Region[J]. Journal of Plant Protection, 2020, 47(3): 609-619. (in Chinese with English abstract)
doi: 10.13802/j.cnki.zwbhxb.2020.2019073
10 刘国霞,谭晴晴,齐军山,等.基于EF-序列位点特异性PCR快速鉴定小麦茎基腐病优势病原菌假禾谷镰孢菌[J].农业生物技术学报,2021,29(5):985-994. DOI:10.3969/j.issn.1674-7968.2021.05.015
LIU G X, TAN Q Q, QI J S, et al. Rapid identification of main pathogen Fusarium graminearum of wheat crown rot using site-specific PCR based on EF- sequence[J]. Journal of Agricultural Biotechnology, 2021, 29(5): 985-994. (in Chinese with English abstract)
doi: 10.3969/j.issn.1674-7968.2021.05.015
11 杜宜新,石妞妞,阮宏椿,等.银川大豆根腐病病原鉴定及种衣剂对其防治效果[J].中国农学通报,2021,37(8):103-109. DOI:10.11924/j.issn.1000-6850.casb2020-0535
DU Y X, SHI N N, RUAN H C, et al. Study on pathogenic fungi causing soybean root rot in Yinchuan and field disease control efficiency of seed coating[J]. Chinese Agricultural Science Bulletin, 2021, 37(8): 103-109. (in Chinese with English abstract)
doi: 10.11924/j.issn.1000-6850.casb2020-0535
12 邱睿,白静科,李成军,等.河南烟草镰刀菌的分子鉴定及致病性分析[J].中国烟草学报,2018,24(2):129-134. DOI:10.16472/j.chinatobacco.2017.367
QIU R, BAI J K, LI C J, et al. Molecular identification and pathogenicity analysis of tobacco Fusarium spp. in Henan[J]. Acta Tabacaria Sinica, 2018, 24(2): 129-134. (in Chinese with English abstract)
doi: 10.16472/j.chinatobacco.2017.367
13 张德珍,李鹏昌,陈晓霞,等.山东省小麦根腐病病原菌的分离鉴定[J].植物保护学报,2016,43(2):233-240. DOI:10.13802/j.cnki.zwbhxb.2016.02.008
ZHANG D Z, LI P C, CHEN X X, et al. Isolation and identification of the pathogens causing wheat common root rot in Shandong Province[J]. Journal of Plant Protection, 2016, 43(2): 233-240. (in Chinese with English abstract)
doi: 10.13802/j.cnki.zwbhxb.2016.02.008
14 吴斌,郭霞,张眉,等.鲁西南地区小麦茎基腐病病原菌鉴定及其致病力分析[J].麦类作物学报,2018,38(3):358-365. DOI:10.7606/j.issn.1009-1041.2018.03.14
WU B, GUO X, ZHANG M, et al. Identification and pathogenicity of pathogens associated with the wheat crown rot in the southwest of Shandong Province[J]. Journal of Triticeae Crops, 2018, 38(3): 358-365. (in Chinese with English abstract)
doi: 10.7606/j.issn.1009-1041.2018.03.14
15 NELSON P E, TOUSSOUN T A, MARASAS W F O. Fusarium Species: An Illustrated Manual for Identification[M]. Pennsylvania, USA: Pennsylvania State University Press, 1983: 206.
16 丛丽丽,康俊梅,张铁军,等.苜蓿镰刀菌根腐病病原菌的分离鉴定与致病性分析[J].草地学报,2017,25(4):857-865. DOI:10.11733/j.issn.1007-043.2017.04.025
CONG L L, KANG J M, ZHANG T J, et al. Identification and pathogenicity test of pathogenic Fusarium of alfalfa root rot[J]. Acta Agrestia Sinica, 2017, 25(4): 857-865. (in Chinese with English abstract)
doi: 10.11733/j.issn.1007-043.2017.04.025
17 AZIL N, STEFAŃCZYK E, SOBKOWIAK S, et al. Identification and pathogenicity of Fusarium spp. associated with tuber dry rot and wilt of potato in Algeria[J]. European Journal of Plant Pathology, 2021, 159(3): 495-509. DOI: 10.1007/s10658-020-02177-5
doi: 10.1007/s10658-020-02177-5
18 WEISS F. Survey for potato wilt in Pennsylvania and southern New York[J]. American Potato Journal, 1924, 1(11): 243-244.
19 RAKHIMOV U K H, KHAKIMOV A K H. Wilt of potatoes in Uzbekistan[J]. Zashchita Ⅰ Karantin Rasteniĭ, 2000, 3: 46.
20 彭学文,朱杰华.河北省马铃薯真菌病害种类及分布[J].中国马铃薯,2008,22(1):31-33. DOI:10.3969/j.issn.1672-3635.2008.01.008
PENG X W, ZHU J H. Types and distribution of potato fungal diseases in Hebei Province[J]. Chinese Potato Journal, 2008, 22(1): 31-33. (in Chinese)
doi: 10.3969/j.issn.1672-3635.2008.01.008
21 王丽丽,日孜旺古丽·苏皮,李克梅,等.乌昌地区马铃薯真菌性病害种类及5种新记录[J].新疆农业科学,2011,48(2):266-270. DOI:10.6048/j.issn.1001-4330.2011.02.013
WANG L L, Riziwangguli·Supi, LI K M, et al. Kinds and five new records of fungus disease of potato in Urumqi and Changji Area in Xinjiang[J]. Xinjiang Agricultural Sciences, 2011, 48(2): 266-270. (in Chinese with English abstract)
doi: 10.6048/j.issn.1001-4330.2011.02.013
22 杨波,郭成瑾,王喜刚,等.新疆马铃薯镰刀菌根腐病发生危害调查及病原菌鉴定[J].西北农业学报,2019,28(12):2069-2077. DOI:10.7606/j.issn.1004-1389.2019.12.020
YANG B, GUO C J, WANG X G, et al. Investigation and pathogen identification of potato root rot caused by Fusarium in Xinjiang[J]. Acta Agriculturae Boreali-Occidentalis Sinica, 2019, 28(12): 2069-2077. (in Chinese with English abstract)
doi: 10.7606/j.issn.1004-1389.2019.12.020
23 王玉琴,杨成德,陈秀蓉,等.甘肃省马铃薯枯萎病(Fusarium avenaceum)鉴定及其病原生物学特性[J].植物保护,2014,40(1):48-53. DOI:10.3969/j.issn.0529-1542.2014.01.008
WANG Y Q, YANG C D, CHEN X R, et al. Identification of potato wilt caused by Fusarium avenaceum and the biological characteristics of its pathogen[J]. Plant Protection, 2014, 40(1): 48-53. (in Chinese with English abstract)
doi: 10.3969/j.issn.0529-1542.2014.01.008
24 O’DONNELL K, KISTLER H C, CIGELNIK E, et al. Multiple evolutionary origins of the fungus causing Panama disease of banana: concordant evidence from nuclear and mitochondrial gene genealogies[J]. PNAS, 1998, 95(5): 2044-2049. DOI: 10.1073/pnas.95.5.2044
doi: 10.1073/pnas.95.5.2044
25 GERLACH W, NIRENBERG H I. The Genus Fusarium: A Pictorial Atlas[M]. Berlin-Dahlem, German: [s. n.], 1982.
26 方中达.植病研究方法[M].北京:中国农业出版社,1998:63.
FANG Z D. Research Method for Plant Pathology[M]. Beijing: China Agriculture Press, 1998: 63. (in Chinese)
27 ZOLAN M E, PUKKILA P J. Inheritance of DNA methylation in Coprinus cinereus [J]. Molecular and Cellular Biology, 1986, 6(1): 195-200.
28 曲延军,蒙美莲,张笑宇,等.马铃薯品种对枯萎病菌的抗性鉴定[J].植物保护,2015,41(3):149-153. DOI:10.3969/j.issn.0529-1542.2015.03.029
QU Y J, MENG M L, ZHANG X Y, et al. Identification of potato resistance to blight caused by Fusarium oxysporum [J]. Plant Protection, 2015, 41(3): 149-153. (in Chinese with English abstract)
doi: 10.3969/j.issn.0529-1542.2015.03.029
29 蓝江林,朱育菁,肖荣凤,等.培养基对尖孢镰刀菌非致病力菌株281产孢量的影响[J].中国农学通报,2007,23(9):415-418. DOI:10.3969/j.issn.1000-6850.2007.09.089
LAN J L, ZHU Y J, XIAO R F, et al. Effects of medium on sporification of Fusarium oxysporum Schl[J]. Chinese Agricultural Science Bulletin, 2007, 23(9): 415-418. (in Chinese with English abstract)
doi: 10.3969/j.issn.1000-6850.2007.09.089
30 李健,李美,高兴祥,等.一种含狗尾草的镰刀菌高效产孢培养基的筛选与优化[J].山东农业科学,2017,49(1):117-120. DOI:10.14083/j.issn.1001-4942.2017.1.025
LI J, LI M, GAO X X, et al. Selection and optimization of an efficient sporulation medium for Fusarium containing Setaria viridis [J]. Shandong Agricultural Sciences, 2017, 49(1): 117-120. (in Chinese with English abstract)
doi: 10.14083/j.issn.1001-4942.2017.1.025
31 杨波,郭成瑾,王喜刚,等.接骨木镰刀菌产孢培养基筛选[J].西北农业学报,2018,27(10):1541-1546. DOI:10.7606/j.issn.1004-1389.2018.10.019
YANG B, GUO C J, WANG X G, et al. Screening a suitable medium for sporulation of Fusaarium sambucinum [J]. Acta Agriculturae Boreali-Occidentalis Sinica, 2018, 27(10): 1541-1546. (in Chinese with English abstract)
doi: 10.7606/j.issn.1004-1389.2018.10.019
32 杨静美,陈健,罗金棠,等.番木瓜茄病镰刀菌的生物学特性研究[J].中国热带农业,2011(1):56-58. DOI:10.3969/j.issn.1673-0658.2011.01.020
YANG J M, CHEN J, LUO J T, et al. Study on the biological characteristics of Fusarium solani of papaya[J]. China Tropical Agriculture, 2011(1): 56-58. (in Chinese)
doi: 10.3969/j.issn.1673-0658.2011.01.020
33 孔琼,王云月,朱有勇,等.香荚兰尖孢镰刀菌生物学特性[J].西南农业学报,2005,18(1):47-49. DOI:10.16213/j.cnki.scjas.2005.01.011
KONG Q, WANG Y Y, ZHU Y Y, et al. Biological characterization of Fusarium oxysporum Schl f. sp. vanillae Gordon[J]. Southwest China Journal of Agricultural Sciences, 2005, 18(1): 47-49. (in Chinese with English abstract)
doi: 10.16213/j.cnki.scjas.2005.01.011
34 李晨芹,李军乔,王鑫慈,等.蕨麻根腐病病原菌的分离鉴定及其生物学特性研究[J].草业学报,2022,31(4):113-123. DOI:10.11686/cyxb2021323
LI C Q, LI J Q, WANG X C, et al. Isolation, identification, and biological characteristics of Fusarium perseae isolated from Potentilla anserina roots[J]. Acta Prataculturae Sinica, 2022, 31(4): 113-123. (in Chinese with English abstract)
doi: 10.11686/cyxb2021323
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