抹茶对高脂饮食诱导的小鼠肝脏脂质积累和炎症反应的保护作用及机制

doi:10.3785/j.issn.1008-9209.2021.07.111

浙江大学学报（农业与生命科学版）

2022, Vol. 48

Issue (4): 525-532 DOI: 10.3785/j.issn.1008-9209.2021.07.111

动物科学与动物医学

抹茶对高脂饮食诱导的小鼠肝脏脂质积累和炎症反应的保护作用及机制

周继红¹(

),余月儿¹,丁乐佳¹,徐平¹,毛立民^1,²,王岳飞¹(

)

^1.浙江大学农业与生物技术学院茶叶研究所，杭州 310058
^2.浙江省茶叶集团股份有限公司，杭州 310058

Protective effect and mechanism of matcha on liver lipid accumulation and inflammatory response induced by high-fat diet in mice

Jihong ZHOU¹(

),Yue'er YU¹,Lejia DING¹,Ping XU¹,Limin MAO^1,²,Yuefei WANG¹(

)

^1.Tea Research Institute, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China
^2.Zhejiang Tea Group Co. , Ltd. , Hangzhou 310058, China

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摘要：

本研究比较分析了4个茶树品种抹茶主要化学成分含量的差异，并选取‘茂绿’品种抹茶对高脂饮食诱导的C57BL/6J肥胖小鼠模型进行0.1%、0.5%、1.0% 3种剂量的膳食补充。结果表明，抹茶能够在不影响摄食量的情况下改善高脂饮食导致的体质量增长、血糖水平升高和肝脏脂质积累，且效果呈现出浓度依赖性。进一步对小鼠肝脏功能、氧化应激水平和炎症反应情况进行检测，发现膳食添加1.0%的抹茶能够显著抑制高脂饮食诱导的肝脏谷丙转氨酶（alanine aminotransferase, ALT）和谷草转氨酶（aspartate aminotransferase, AST）活性异常升高，提升抗氧化酶活性，降低炎症因子表达水平，并下调Toll样受体4（Toll-like receptor 4, TLR4）和下游MyD88基因的表达。综上所述，抹茶能够有效改善食源性肥胖和高脂饮食诱发的肝脏脂肪性病变与炎症反应，对TLR4/MyD88信号通路的抑制是其潜在的作用机制。

关键词： 抹茶; 肥胖; 非酒精性脂肪性肝病; 氧化应激; 炎症因子

Abstract:

In this study, we analyzed the main chemical components of matcha made from four tea cultivars, established a high-fat diet-induced C57BL/6J obese mouse model, and selected ‘Maolü’ matcha as an experimental dietary supplement with three doses of 0.1%, 0.5% and 1.0%. The results showed that matcha could reduce body mass gain, blood glucose level rise and liver lipid accumulation induced by the high-fat diet without affecting food intake, and the effect was concentration-dependent. Furthermore, we detected the liver function, oxidative stress level and inflammatory response in mice, and the results showed that dietary supplementation of 1.0% matcha significantly inhibited the abnormal increase of activities of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) induced by a high-fat diet in liver, and increased the activity of antioxidant enzyme. The expression levels of inflammatory factors, Toll-like receptor 4 (TLR4) and MyD88 were also significantly reduced. In conclusion, matcha effectively improves obesity-related fatty liver lesions and inflammation, and its potential mechanism is to inhibit the activation of the TLR4/MyD88 signaling pathway.

Key words: matcha obesity non-alcoholic fatty liver disease oxidative stress inflammatory cytokines

收稿日期: 2021-07-11 出版日期: 2022-09-03

CLC:

S 571.1

基金资助: 中国博士后科学基金第68批面上项目(2020M681868)

通讯作者: 王岳飞 E-mail: zhoujihong@zju.edu.cn;zdcy@zju.edu.cn

作者简介: 周继红（https://orcid.org/0000-0003-2315-8612），E-mail：zhoujihong@zju.edu.cn

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引用本文:

周继红,余月儿,丁乐佳,徐平,毛立民,王岳飞. 抹茶对高脂饮食诱导的小鼠肝脏脂质积累和炎症反应的保护作用及机制[J]. 浙江大学学报（农业与生命科学版）, 2022, 48(4): 525-532.

Jihong ZHOU,Yue'er YU,Lejia DING,Ping XU,Limin MAO,Yuefei WANG. Protective effect and mechanism of matcha on liver lipid accumulation and inflammatory response induced by high-fat diet in mice. Journal of Zhejiang University (Agriculture and Life Sciences), 2022, 48(4): 525-532.

链接本文:

https://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2021.07.111 或 https://www.zjujournals.com/agr/CN/Y2022/V48/I4/525

表1 实时荧光定量PCR引物序列

表2 不同品种抹茶主要生化成分含量 (%)

表3 不同品种抹茶儿茶素单体含量 (%)

表4 抹茶对不同组小鼠体质量、摄食量以及空腹血糖水平的影响

图1 抹茶对不同组小鼠肝脏脂质积累的影响在HE染色中，蓝色为肝脏细胞核，红色为细胞质，未着色的脂肪在显微镜图中呈现白色空泡；在油红O染色中，蓝色为肝脏细胞核，红色为脂肪。

图 2 抹茶对不同组小鼠肝脏功能与氧化应激水平的影响短栅上不同小写字母表示在P＜0.05水平差异有统计学意义。

图 3 抹茶对不同组小鼠肝脏炎症因子和 TLR4/MyD88 通路基因表达的影响短栅上不同小写字母表示同种炎症因子或基因在不同处理组间在P＜0.05水平差异有统计学意义。

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