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浙江大学学报（农业与生命科学版）
浙江大学学报(农业与生命科学版)  2015, Vol. 41 Issue (2): 195-200    DOI: 10.3785/j.issn.1008-9209.2014.09.192
食品科学     
丁酸钠对饮食诱导肥胖大鼠的作用
朱涵1， 谭莎莎1， 杨虹2， 王凌1*
1.华中农业大学食品科学技术学院，环境食品学教育部重点实验室，武汉 430070；2.武汉市普爱医院，武汉 430030
Effect of sodium butyrate on diet-induced obesity in the Sprague-Dawley rat.
Zhu Han1, Tan Shasha1, Yang Hong2, Wang Ling1*
(1. Key Laboratory of Environment Correlative Dietology, Ministry of Education, College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; 2. Pu’ai Hospital of Wuhan, Wuhan 430030, China)
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摘要： 为建立由高脂饲料诱导的肥胖易感（obesity-susceptible, OS）和肥胖抵抗（obesity-resistant, OR）大鼠模型,并探讨在高脂饲料中添加丁酸钠对二者的作用，给予SD(Sprague-Dawley)大鼠高脂饲料饲喂3周后依据其体质量增加量排序并再分组，上1/3作为OS组，下1/3作为OR组，继续给予高脂饲料喂养12周，OS大鼠体质量增加明显，体质指数、皮下脂肪、内脏脂肪和体脂肪含量以及血清总胆固醇、甘油三酯、低密度脂蛋白、瘦素与对照组比较显著增高，差异有统计学意义（P0.05）。按照此造模方法从饲喂高脂饲料第4周起，丁酸钠干预（obesity intervention with sodium butyrate, OI）组增加8 mmol/L丁酸钠生理盐水（0.9%氯化钠）溶液1 mL 灌胃，1次/日；肥胖对照（obesity control, OC）组则用1 mL 0.9%氯化钠溶液灌胃，1次/日，共干预16周。结果表明：添加丁酸钠后，OI组大鼠体质量增加、体质指数、皮下脂肪、内脏脂肪和体脂肪含量以及血清总胆固醇、甘油三酯、低密度脂蛋白、瘦素与OC组比较均显著改善，差异有统计学意义（P<0.05）。说明SD大鼠对于高脂饲料具有不同的应答能力，可获得肥胖敏感及肥胖抵抗模型；对于高脂饮食敏感的SD大鼠，在其高脂饲料中添加丁酸盐能够改善其体质量增加与体质指数，减少体脂肪含量，降低血脂与血清瘦素水平。
Abstract: Obesity has been reported as an increasingly prevalent and highly heritable health problem leading to increased risks for several common diseases. Human obesity can be induced by genetic factors such as loss-of-function mutations in individual genes. The products of these genes are essential for normal body mass regulation in both laboratory animals and humans. Nevertheless, the role of gene-environment interactions in the etiology of obesity cannot be ignored. Diet is a major factor of our current obesogenic environment, and the interests have been aroused in rodent models of diet-induced obesity (DIO). The SD (Sprague-Dawley) rat model of DIO was been reported to exhibit a clear segregation into susceptible and resistant subpopulations shortly after being transferred to a high energy diet. We established the obesity-susceptible (OS) and obesity-resistant (OR) rat model and examined the effect of butyric acid, a short chain fatty acid formed by fermentation in the large intestine, in the regulation of obesity in mice fed a high-fat diet. Male SD rats were divided into OS rats and OR rats after being fed with high fat diet for three weeks. Then both of them were fed with high fat diet for another 12 weeks. Measurements of body mass, body length, Lee’s index, and body mass index (BMI) were performed. After the rats were sacrificed, body fat content, serum total cholesterol (TC), triglyceride (TG), high density lipoprotein (HDL), low density lipoprotein (LDL) and serum leptin were measured. The results showed that there were significant differences in body mass, BMI, body fat content, TC, TG, LDL and serum leptin between OS rats and control rats. But there were no significant differences between OR rats and control rats. In the OS rats, sodium butyrate was administrated in the high-fat diet at the concentration of 8 mmol/L for 16 weeks. Body mass, body length, Lee’s index, BMI, body fat content, TC, TG, HDL, LDL and serum leptin were investigated both in obesity intervention with sodium butyrate (OI) group and obesity control (OC) group. The values of body mass, BMI, body fat content, TC, TG, LDL and serum leptin for OI rats were significantly lower than OC rats. But there were no significant differences between OC rats and normal control rats. In conclusion, the supplementation of sodium butyrate in the high-fat diet prevents the development of obesity in the obesity-susceptible SD rat.
出版日期: 2015-03-20
CLC:  R 151.3  
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朱涵
谭莎莎
杨虹
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引用本文:

朱涵，谭莎莎，杨虹，王凌. 丁酸钠对饮食诱导肥胖大鼠的作用[J]. 浙江大学学报(农业与生命科学版), 2015, 41(2): 195-200.

Zhu Han, Tan Shasha, Yang Hong, Wang Ling . Effect of sodium butyrate on diet-induced obesity in the Sprague-Dawley rat.. Journal of Zhejiang University (Agriculture and Life Sciences), 2015, 41(2): 195-200.

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http://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2014.09.192    或    http://www.zjujournals.com/agr/CN/Y2015/V41/I2/195

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