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浙江大学学报(农业与生命科学版)  2022, Vol. 48 Issue (2): 154-162    DOI: 10.3785/j.issn.1008-9209.2021.03.231
园艺科学     
葡萄新品种南太湖特早果实发育过程中特征香气物质的变化规律
奚昕琰1(),杨淑娜1,王莉2,殷益明2,贾惠娟1()
1.浙江大学农业与生物技术学院果树科学研究所,杭州 310058
2.湖州市农业科技发展中心,浙江 湖州 313000
Variation rule of characteristic aroma compounds during the berry development process of a new grape cultivar ‘Nantaihutezao’
Xinyan XI1(),Shuna YANG1,Li WANG2,Yiming YIN2,Huijuan JIA1()
1.Institute of Fruit Science, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China
2.Huzhou Agricultural Science and Technology Development Center, Huzhou 313000, Zhejiang, China
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摘要:

以自主选育的葡萄新品种‘南太湖特早’及其母本‘夏黑’的果实为实验材料,重点比较了二者发育过程中挥发性特征香气物质的变化规律,并对二者的果粒质量、固酸比等果实品质指标进行了评价。结果表明:‘南太湖特早’比‘夏黑’提早7 d左右成熟,且其果实中具有花果香的萜烯类和酮类物质含量较高,在果皮和果肉中分别提升了162%和233%;在‘南太湖特早’果实发育过程中,果皮中积累的特征香气物质含量呈现先快速上升后缓慢下降的趋势,且峰值较‘夏黑’更高且出现更早;2个品种葡萄果肉中1-辛烯-3-酮、β-大马酮、柠檬烯的含量变化规律存在显著差异,‘夏黑’表现为在果实发育中期保持高含量、后期快速降解,‘南太湖特早’则表现为在中期以低水平增长、后期较少降解。本研究结果为葡萄优新良品的香味遗传育种提供了理论依据。

关键词: ‘南太湖特早’葡萄果实发育香气物质变化规律    
Abstract:

Taking the berries of the new grape cultivar ‘Nantaihutezao’ and its maternal cultivar ‘Summer Black’ as experimental materials, the variation rule of volatile characteristic aroma compounds during the berry development processes was compared, and their berry quality indicators such as the mass of berry particles, solid-acid ratio were evaluated. The results showed that ‘Nantaihutezao’ matured about 7 d earlier than ‘Summer Black’. The contents of terpenes and ketones which distributed floral and fruity aroma in ‘Nantaihutezao’ were significantly higher than those in ‘Summer Black’, which increased by 162% and 233% in the peel and pulp, respectively. During the berry development process of ‘Nantaihutezao’, the contents of characteristic aroma compounds accumulated in peel first increased rapidly and then decreased slowly, and the peak values were higher and appeared earlier than those of ‘Summer Black’. There were significant differences in the contents of 1-octen-3-one, β-damascenone and limonene in the pulp of the two grape cultivars. The contents of ‘Summer Black’ maintained at a high level in the middle of maturity and rapidly degraded in the later period; by contrast, the contents of ‘Nantaihutezao’ increased slowly in the middle and less degraded in the later period. The above results provide a theoretical basis for the aroma genetic breeding of excellent and new grape cultivars.

Key words: ‘Nantaihutezao’ grape    berry development    aroma compounds    variation rule
收稿日期: 2021-03-23 出版日期: 2022-04-29
CLC:  S 663.1  
基金资助: 浙江省农业农村厅“三农六方”科技协作项目(CTZB-F170623LWZ-SNY1)
通讯作者: 贾惠娟     E-mail: 3150100260@zju.edu.cn;jiahuijuan@zju.edu.cn
作者简介: 奚昕琰(https://orcid.org/0000-0003-4791-489X),E-mail:3150100260@zju.edu.cn
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引用本文:

奚昕琰,杨淑娜,王莉,殷益明,贾惠娟. 葡萄新品种南太湖特早果实发育过程中特征香气物质的变化规律[J]. 浙江大学学报(农业与生命科学版), 2022, 48(2): 154-162.

Xinyan XI,Shuna YANG,Li WANG,Yiming YIN,Huijuan JIA. Variation rule of characteristic aroma compounds during the berry development process of a new grape cultivar ‘Nantaihutezao’. Journal of Zhejiang University (Agriculture and Life Sciences), 2022, 48(2): 154-162.

链接本文:

https://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2021.03.231        https://www.zjujournals.com/agr/CN/Y2022/V48/I2/154

图1  ‘南太湖特早’和‘夏黑’果实生长发育曲线实线箭头表示‘南太湖特早’果实开始转色,虚线剪头表示‘夏黑’果实开始转色。

化合物种类

Compound type

‘夏黑’果皮

Peel of ‘Summer Black’

‘南太湖特早’果皮

Peel of ‘Nantaihutezao’

‘夏黑’果肉

Pulp of ‘Summer Black’

‘南太湖特早’果肉

Pulp of ‘Nantaihutezao’

数量

Number

相对含量

Relative

content/%

数量

Number

相对含量

Relative

content/%

数量

Number

相对含量

Relative

content/%

数量

Number

相对含量

Relative

content/%

总量 Total content/(μg/kg)2 947.844 510.75665.36926.63
醛类 Aldehydes883.16969.01880.61854.81
萜烯类 Terpenes1311.621519.401012.42931.81
醇类 Alcohols41.2567.3342.1746.24
酮类 Ketones30.4351.2352.2563.26
酯类 Esters62.0851.6831.1952.34
酚类 Phenols10.9410.5700.0000.00
其他 Others10.5120.7811.3611.54
总计 Total3699.9943100.0031100.0033100.00
表1  ‘南太湖特早’和‘夏黑’成熟果实中的挥发性香气物质

香气物质

Aroma compound

香味描述

Odor description

香气阈值

Odor

threshold/(μg/L)

果皮香气活性值

Peel OAV

果肉香气活性值

Pulp OAV

‘南太湖特早’

‘Nantaihutezao’

‘夏黑’

‘Summer

Black’

‘南太湖特早’

‘Nantaihutezao’

‘夏黑’

‘Summer

Black’

酮类 Ketones

1-辛烯-3-酮

1-octen-3-one

蔬菜味,鸡肉味0.0032 371.60a1 911.00b

β-大马酮

β-damascenone

甜香,果香,花香,蜂蜜味,烤苹果味0.0025 047.44a868.69c2 058.48b547.34d
萜烯类 Terpenes
柠檬烯 Limonene水果味,柠檬味1010.59b9.61b19.81a0.89c
芳樟醇 Linalool花香,荔枝味,玫瑰香味0.5190.89a32.47b7.45c
香茅醇 Citronellol玫瑰香味106.50a1.16b
醛类 Aldehydes
己醛 Hexanal绿色蔬菜味4.5141.88a147.81a42.08b47.53b

反式-2-己烯醛

(E)-2-hexenal

青草味17139.55a99.39b14.56c15.14c
癸醛 Decanal肥皂味,牛脂味0.1127.08a85.10c100.82b81.49c

反式-2-壬烯醛

(E)-2-nonenal

脂肪味0.065109.19a77.58bc75.98c82.49b
壬醛 Nonanal脂肪味,青菜味,果味137.05bc58.29a38.41bc40.40b

苯乙醛

Phenylacetaldehyde

花香,玫瑰香味46.14a1.00c1.67b0.26d
庚醛 Heptanal青草味32.35a2.08b1.76c1.52d
其他 Others
伞花烃 Cymene柑橘味,青菜味11.42.86a1.32b1.25b0.79c
表2  ‘南太湖特早’和‘夏黑’成熟果实中特征香气物质的香气活性值
图2  果实发育过程中4类特征香气物质含量的变化A.‘夏黑’;B.‘南太湖特早’。
图3  7种主要特征香气物质在果实发育过程中的含量变化
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