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浙江大学学报（农业与生命科学版）
浙江大学学报(农业与生命科学版)  2011, Vol. 37 Issue (3): 237-354    DOI: 10.3785/j.issn.1008-9209.2011.03.001
生物科学与技术     
番茄生长素应答因子SlARF8-1的分离与SlARFs表达特征分析
王飞燕,吴健,张佳景,程琳,孔福苓,彭真,卢钢
浙江大学农业与生物技术学院园艺系, 浙江 杭州 310029
Identification of auxin response factor SlARF8-1 gene and characterization of expression pattern of some SlARFs in Solanum lycopersicum
WANG Fei-yan,WU Jian,ZHANG Jia-jing,CHENG Lin,KONG Fu-ling,PENG Zhen,LU Gang
Department o f Horticulture , College of A griculture & Biotechnology , Zhej iang University ,Hangzhou 310029 , China
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摘要： 根据番茄基因组序列,以cDNA 为模板扩增获得1个生长素应答因子新基因,其与番茄SlARF8 基因同源性高达94％ ,编码区全长2535 bp ,编码844个氨基酸,但这2个基因存在于番茄的不同染色体上,是区别于SlARF8 的一个新基因,将其命名为SLARF8-1 ,NCBI 登录号为HM560979.利用半定量反转录酶‐聚合酶链反应( reverse transcription‐polymerase chain reaction , RT‐PCR)分析4种ARF 基因( SlARF3 、SlARF4 、SlARF6 和SlARF8-1)的时空表达特性,发现SlA RF8-1 基因在"中蔬6号"番茄的5个器官(茎、叶、花前子房、花后子房和不同时期果实)中均有表达;SlARF3 在叶片和子房中几乎没有表达;SlARF6 在番茄叶片中表达量低,而在果实中的表达量较高;在果实发育的不同时期,4个SlARFs 的表达量变化不大.利用2 ,4‐二氯苯氧乙酸(2 ,4‐D)和赤霉酸(GA3)处理番茄花蕾均可诱导单性结实,但外源激素并没有引起果实内SlARFs 表达水平的显著改变,4个SlARFs 基因在番茄单性果实中均有表达.
Abstract: The full-length cDNA sequence of a new auxin response factor family gene with an open reading frame (ORF) of  2535 bp in length was cloned , encoding a protein of 844 amino acids that was 94% homologous with SlARF8 , and this new gene located on different chromosomes with SlARF8 based on tomato genome data , so it was named SlA RF8-1 ( GenBank accession number HM560979 ) .Then the temporal and spatial expression patterns of four A RFs genes ( SlARF3 , SlARF4 , SlARF6 and SlARF8-1) were analyzed in tomato stems , leaves , ovaries and fruits at different development stage using semi-quantitative RT-PCR . The results showed that SlA RF8-1 was expressed in all tissues examined , but at a lower level in the ovary after pollination .By contrast , the transcripts of SlA RF3 and SlA RF6 were undetectable in leaf and ovary . These four SlA RFs genes showed similar expressiontomato fruits didn‘t change significantly after the ovaries were treated with exogenous hormones of 2 ,4‐dichlorophenoxyacetic acid (2 ,4‐D) and gibberellic acid ( GA3 ) , which could induce significantly parthenocarpic fruits in tomato .
出版日期: 2011-05-20
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孔福苓
彭真
卢钢
程琳
王飞燕
吴健
张佳景

引用本文:

王飞燕,吴健,张佳景,程琳,孔福苓,彭真,卢钢. 番茄生长素应答因子SlARF8-1的分离与SlARFs表达特征分析[J]. 浙江大学学报(农业与生命科学版), 2011, 37(3): 237-354.

WANG Fei-yan,WU Jian,ZHANG Jia-jing,CHENG Lin,KONG Fu-ling,PENG Zhen,LU Gang. Identification of auxin response factor SlARF8-1 gene and characterization of expression pattern of some SlARFs in Solanum lycopersicum. Journal of Zhejiang University: Agric. & Life Sci., 2011, 37(3): 237-354.

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http://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2011.03.001    或    http://www.zjujournals.com/agr/CN/Y2011/V37/I3/237

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