浙江省2市动物源大肠埃希菌及肠球菌耐药性比较和分析

doi:10.3785/j.issn.1008-9209.2020.11.121

浙江大学学报（农业与生命科学版）

2021, Vol. 47

Issue (5): 647-659 DOI: 10.3785/j.issn.1008-9209.2020.11.121

动物科学与动物医学

浙江省2市动物源大肠埃希菌及肠球菌耐药性比较和分析

唐标^1,²(

),郝运^1,²,林家辉³,王静鸽^2,⁴,吉小凤^1,²,钱鸣蓉^1,²,杨华^1,²(

)

^1.浙江省农业科学院农产品质量安全危害因子与风险防控国家重点实验室，杭州 310021
^2.浙江省农业科学院农产品质量安全与营养研究所，杭州 310021
^3.浙江理工大学生命科学与医药学院，杭州 310018
^4.青海大学农牧学院，西宁 810016

Comparison and analysis of antimicrobial resistance of Escherichia coli and Enterococcus isolated from animals in Jinhua City and Taizhou City of Zhejiang Province

Biao TANG^1,²(

),Yun HAO^1,²,Jiahui LIN³,Jingge WANG^2,⁴,Xiaofeng JI^1,²,Mingrong QIAN^1,²,Hua YANG^1,²(

)

^1.State Key Laboratory of Quality Safety and Risk Factor Prevention and Control of Agricultural Products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
^2.Institute of Agro-products Safety and Nutrition, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
^3.College of Life Science and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, China
^4.College of Agriculture and Animal Husbandry, Qinghai University, Xining 810016, China

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摘要：

为了解2020年浙江省金华市和台州市动物源细菌耐药情况，于2020年5月分别从这2市7个畜禽养殖场随机采集畜禽肛拭子样品284份，使用选择性培养基分离大肠埃希菌和肠球菌，通过基质辅助激光解吸电离飞行时间质谱（matrix-assisted laser desorption ionization time-of-flight mass spectrometry, MALDI-TOF-MS）对其进行鉴定，并使用微量肉汤稀释法检测这2种细菌的最小抑菌浓度（minimal inhibitory concentration, MIC）。结果显示：金华市大肠埃希菌对四环素的耐药率最高（87.5%），其次是对磺胺异噁唑（81.2%），对黏菌素的耐药率最低（0%）；台州市大肠埃希菌对磺胺异噁唑的耐药率最高（97.1%），对美罗培南和黏菌素的耐药率均为0%。金华和台州2市肠球菌对磺胺异噁唑耐药率均为100%，其次是对泰妙菌素，均为98.6%，2市未检测到对奥格门汀和万古霉素耐药的肠球菌。此外，从整体耐药率、MIC分布和耐药谱型上看，台州市的细菌耐药性水平比金华市的高。总之，通过对2个地级市的畜禽养殖中细菌耐药性水平进行监测和对比发现，2市的动物源大肠埃希菌和肠球菌耐药水平较高且存在差异。因此，通过农业主管部门的兽药减量化行动，持续监测耐药性动态变化十分必要。

关键词： 畜禽; 大肠埃希菌; 肠球菌; 耐药性; 金华市; 台州市

Abstract:

To investigate the antimicrobial resistance of bacteria from animals in Jinhua City and Taizhou City of Zhejiang Province in May of 2020, a total of 284 anal swab samples were randomly collected from seven livestock and poultry farms in the two cities, respectively. Escherichia coli and Enterococcus were isolated by selective culture media and were identified by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS). The minimal inhibitory concentration (MIC) was determined by broth microdilution method. The results showed that the antimicrobial resistance rate of E. coli to tetracycline was the highest (87.5%), followed by sulfamethoxazole (81.2%), while the antimicrobial resistance rate to colistin was the lowest (0%) in Jinhua City. In addition, the antimicrobial resistance rate of E. coli to sulfamethoxazole was the highest (97.1%), and the resistance rates to meropenem and colistin were both 0% in Taizhou City. The antimicrobial resistance rate of Enterococcus to sulfamethoxazole was 100% in both cities, followed by tamoxifen (98.6%). No amoxicillin/clavulanate and vancomycin resistant Enterococcus was found in the two cities. In terms of the overall antimicrobial resistance rate, MIC distribution and antimicrobial resistance pattern, the level of antimicrobial resistance in Taizhou was higher than that in Jinhua. In conclusion, through monitoring and comparing the level of antimicrobial resistance in livestock and poultry breeding in two cities, it is found that the levels of antimicrobial resistance of E. coli and Enterococcus from animals are high and different. Therefore, continuous surveillance of antimicrobial resistance dynamics is essential through veterinary antibiotics reduction initiatives by agricultural authorities.

Key words: livestock and poultry Escherichia coli Enterococcus antimicrobial resistance Jinhua City Taizhou City

收稿日期: 2020-11-12 出版日期: 2021-10-27

CLC:

S 852.6

基金资助: 浙江省重点研发计划(2020C02031);国家自然科学基金青年科学基金(31700007);省部共建农产品质量安全危害因子与风险防控国家重点实验室项目(2010DS700124-ZZ2008)

通讯作者: 杨华 E-mail: tb_411@163.com;yanghua@zaas.ac.cn

作者简介: 唐标（https://orcid.org/0000-0002-3605-4022），E-mail：tb_411@163.com

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引用本文:

唐标,郝运,林家辉,王静鸽,吉小凤,钱鸣蓉,杨华. 浙江省2市动物源大肠埃希菌及肠球菌耐药性比较和分析[J]. 浙江大学学报（农业与生命科学版）, 2021, 47(5): 647-659.

Biao TANG,Yun HAO,Jiahui LIN,Jingge WANG,Xiaofeng JI,Mingrong QIAN,Hua YANG. Comparison and analysis of antimicrobial resistance of Escherichia coli and Enterococcus isolated from animals in Jinhua City and Taizhou City of Zhejiang Province. Journal of Zhejiang University (Agriculture and Life Sciences), 2021, 47(5): 647-659.

链接本文:

http://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2020.11.121 或 http://www.zjujournals.com/agr/CN/Y2021/V47/I5/647

图1 质控菌株的基质辅助激光解吸电离飞行时间质谱鉴定图谱

图2 金华市和台州市大肠埃希菌的耐药率比较AMP：氨苄西林；A/C：奥格门汀；GEM：庆大霉素；SPT：大观霉素；TET：四环素；FFC：氟苯尼考；SF：磺胺异噁唑；SXT：复方新诺明； CEF：头孢噻呋；CAZ：头孢他啶；ENR：恩诺沙星；OFL：氧氟沙星；MEM：美罗培南；CL：黏菌素。

图3 金华市和台州市16种抗生素对大肠埃希菌的MIC分布AMP：氨苄西林；A/C：奥格门汀；GEM：庆大霉素；SPT：大观霉素；TET：四环素；FFC：氟苯尼考；SF：磺胺异噁唑；SXT：复方新诺明； CEF：头孢噻呋；CAZ：头孢他啶；ENR：恩诺沙星；OFL：氧氟沙星；MEM：美罗培南；APR：安普霉素；CL：黏菌素；MEQ：乙酰甲喹。

图4 金华市和台州市肠球菌的耐药率比较PEN：青霉素；A/C：奥格门汀；ERY：红霉素；ENR：恩诺沙星；SF：磺胺异噁唑；VAN：万古霉素；DOX：多西环素；FFC：氟苯尼考；TIA：泰妙菌素；TIL：替米考星；LZD：利奈唑胺。

图5 金华市和台州市27种抗生素对肠球菌的MIC分布PEN：青霉素；A/C：奥格门汀；ERY：红霉素；CLI：克林霉素；ENR：恩诺沙星；OFL：氧氟沙星；CEF：头孢噻呋；CFX：头孢西丁；SF：磺胺异噁唑；OXA：苯唑西林；VAN：万古霉素；SXT：复方新诺明；DOX：多西环素；FFC：氟苯尼考；TIA：泰妙菌素；TIL：替米考星；GEM：庆大霉素；LZD：利奈唑胺；BAC：杆菌肽；TET：四环素；KIT：吉他霉素；FLA：黄霉素；EDC：恩拉霉素；QCT：喹烯酮；NOS：那西肽；AVI：阿维拉霉素；VGM：弗吉尼亚霉素。

Table 1 Antimicrobial resistance patterns of E. coli isolated from Jinhua City

表2 台州市大肠埃希菌的耐药谱

表3 金华市肠球菌的耐药谱

表4 台州市肠球菌的耐药谱

表1 金华市大肠埃希菌的耐药谱

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