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浙江大学学报（农业与生命科学版）
浙江大学学报（农业与生命科学版）  2020, Vol. 46 Issue (4): 441-448    DOI: 10.3785/j.issn.1008-9209.2020.02.011
园艺学     
不同光源处理对栀子叶片叶绿素荧光参数和生化成分的影响
张亚芬(),杨晖
丽水学院生态学院，浙江 丽水 323000
Effects of different artificial lights on chlorophyll fluorescence parameters and biochemical components of Gardeniajasminoides Ellis leaves
Yafen ZHANG(),Hui YANG
College of Ecology, Lishui University, Lishui 323000, Zhejiang, China
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摘要：

以2种发光二极管（light emitting diode, LED）和荧光灯为光源，在人工气候室条件下探讨3种光源处理对栀子叶片叶绿素荧光参数和生化成分的影响。结果表明：随着处理时间的增加，3种光源显著增加了栀子叶片的叶绿素指数和氮平衡指数，但3种光源之间无显著性差异。在红蓝光质比最大（4.4）的LED-2照射下，栀子叶片多酚和游离氨基酸含量最高；在具有最低的红蓝光质比（0.8）和最高的绿光占比（0.41）荧光灯照射下，栀子叶中类黄酮指数最高；而在蓝光比例较高的LED-1照射下，栀子叶生化成分含量及实际光化学量子效率较低。以上研究结果表明：在开发适合栀子生长的LED灯时，应适当增加部分绿光及远红光，降低红光与远红光比值，以促进栀子叶中类黄酮、多酚、游离氨基酸等功能成分的积累。
关键词： 发光二极管;  绿光;  光化学量子效率;  栀子;  类黄酮;  游离氨基酸    
Abstract:

The effects of two light emitting diodes（LED-1 and LED-2）and the fluorescent lamp treatments on chlorophyll fluorescence parameters and biochemical components of Gardenia jasminoides Ellis leaves were studied in the artificial climate chamber. The results showed that with the increase of the treatment time, the nitrogen balance index and chlorophyll index increased significantly, while no significant differences among the three light source treatments. At the end of the experiment, the highest contents of polyphenols and free amino acids were recorded under the LED-2 treatment with the maximal ratio of red to blue light (4.4). The highest flavonoid index was detected under the fluorescent lamp treatment with the lowest ratio of red to blue light (0.8) and the highest ratio of green light (0.41). The minimal values of biochemical component contents and actual photochemical quantum efficiency were observed under the LED-1 treatment with a higher ratio of blue light. The above results indicate that, in order to promote the accumulation of flavonoid and free amino acids in G. jasminoides leaves, it is necessary to increase account of green and far-red lights, and reduce the ratio of red to far-red light, while designing a suitable LED for G. jasminoides.
Key words: light emitting diode    green light    photochemical quantum efficiency    Gardenia jasminoides Ellis    flavonoid    free amino acid
收稿日期: 2020-02-01 出版日期: 2020-09-11
CLC:  S 685.99  
基金资助: 浙江省自然科学基金(LY20C030002)
通讯作者: 张亚芬     E-mail: zhangyafen@lsu.edu.cn
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引用本文:

张亚芬,杨晖. 不同光源处理对栀子叶片叶绿素荧光参数和生化成分的影响[J]. 浙江大学学报（农业与生命科学版）, 2020, 46(4): 441-448.

Yafen ZHANG,Hui YANG. Effects of different artificial lights on chlorophyll fluorescence parameters and biochemical components of Gardeniajasminoides Ellis leaves. Journal of Zhejiang University (Agriculture and Life Sciences), 2020, 46(4): 441-448.

链接本文:

http://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2020.02.011    或    http://www.zjujournals.com/agr/CN/Y2020/V46/I4/441

光源

Light source

峰值波长

Peak

wavelength/nm

蓝光占比

Blue light

proportion/%

绿光占比

Green light

proportion/%

红光占比

Red light

proportion/%

远红光占比

Far-red light

proportion/%

红蓝光质比

Ratio of red

to blue light

红光/远红光质比

Ratio of red to

far-red light

荧光灯（对照）

Fluorescent lamp (control)

54532412520.811.4
LED-144930274221.426.5
LED-266012365024.422.1
表1  试验用人工光源的光谱参数
图1  3种光源的绝对光谱分布
图2  不同光源处理下栀子叶生长指数的变化短栅上不同小写字母表示相同光照条件下不同处理时间在P＜0.05水平差异有统计学意义；短栅上不同大写字母表示不同光照处理下相同处理时间在P＜0.05水平差异有统计学意义。
图3  不同处理下栀子叶绿素荧光参数的变化短栅上不同小写字母表示相同光照条件下不同处理时间在P＜0.05水平差异有统计学意义；短栅上不同大写字母表示不同光照处理下相同处理时间在P＜0.05水平差异有统计学意义。

光源

Light source

含水量

Water content/%

多酚

Total polyphenols/%

类黄酮

Flavonoid/(mg/g)

游离氨基酸

Free amino acid/%

荧光灯（对照）

Fluorescent lamp (control)

67.15±0.97aA4.76±0.13bA37.26±1.42aA0.097±0.010bB
LED-166.81±1.66aA4.52±0.12cB31.48±1.31bB0.092±0.005bB
LED-266.69±0.73aA4.95±0.15aA32.97±2.10bB0.107±0.008aA
表2  不同光源处理21 d后对栀子叶片生理生化指标的影响
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