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浙江大学学报（农业与生命科学版）
浙江大学学报（农业与生命科学版）  2020, Vol. 46 Issue (1): 8-16    DOI: 10.3785/j.issn.1008-9209.2019.10.151
综述     
定量蛋白质组学在果蔬采后商品化处理中的研究现状及进展
梁泽1(),王蕾1,杨明依1,罗自生1,2,3,徐艳群1,3,李莉1,2,3()
1.浙江大学生物系统工程与食品科学学院，杭州 310058
2.浙江大学，农业农村部农产品产后处理重点实验室，杭州 310058
3.浙江大学宁波研究院，浙江 宁波 315100
Status and progress in quantitative proteomic study of postharvest fruits and vegetables during commercial treatment
Ze LIANG1(),Lei WANG1,Mingyi YANG1,Zisheng LUO1,2,3,Yanqun XU1,3,Li LI1,2,3()
1.College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China
2.Key Laboratory of Agro-Products Postharvest Handling, Ministry of Agriculture and Rural Affairs, Zhejiang University, Hangzhou 310058, China
3.Ningbo Research Institute, Zhejiang University, Ningbo 315100, Zhejiang, China
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摘要：

果蔬采后商品化处理涉及成熟衰老等多种生理和生化反应，利用高通量蛋白质组学技术对蛋白质进行定性和定量分析，识别果蔬成熟衰老过程中重要的蛋白质及其功能，能够对果实成熟衰老、抗逆性、致敏性、外源处理调控机制等进行深入研究。本文综述了定量蛋白质组学技术在果蔬采后商品化处理所涉及的生理生化过程及外源调控机制中的研究现状及进展，对果蔬采后商品化处理中保质保鲜研究具有重要指导意义。
关键词： 蛋白质组学;  果蔬;  商品化处理;  现状;  进展    
Abstract:

The postharvest commercial treatment of fruits and vegetables involves a variety of physiological and biochemical reactions such as ripening and senescence processes. The qualitative and quantitative analysis of proteins by using high-throughput proteomics can identify important proteins and their functions in the ripening and senescence process of fruits and vegetables, so that in-depth studies can be conducted on the regulation mechanism of fruit ripening and senescence, stress resistance, allergy and the response to exogenous treatment. This paper reviewed the physiological and biochemical changes of fruits and vegetables and the molecular mechanism of regulation after harvest by quantitative proteomics, which provides the guiding significance for the preservation study of fruits and vegetables during postharvest commercial treatment.
Key words: proteomics    fruits and vegetables    commercial treatment    status    progress
收稿日期: 2019-10-15 出版日期: 2020-02-25
CLC:  TS 255.3  
基金资助: “十三五”国家重点研发计划专项(2017YFD0401304)
通讯作者: 李莉     E-mail: liangze0803@zju.edu.cn;lili984@zju.edu.cn
作者简介: 梁泽（https://orcid.org/0000-0002-3780-1157），E-mail：liangze0803@zju.edu.cn
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梁泽
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引用本文:

梁泽,王蕾,杨明依,罗自生,徐艳群,李莉. 定量蛋白质组学在果蔬采后商品化处理中的研究现状及进展[J]. 浙江大学学报（农业与生命科学版）, 2020, 46(1): 8-16.

Ze LIANG,Lei WANG,Mingyi YANG,Zisheng LUO,Yanqun XU,Li LI. Status and progress in quantitative proteomic study of postharvest fruits and vegetables during commercial treatment. Journal of Zhejiang University (Agriculture and Life Sciences), 2020, 46(1): 8-16.

链接本文:

http://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2019.10.151    或    http://www.zjujournals.com/agr/CN/Y2020/V46/I1/8

品种

Crops

技术策略

Technical strategies

蛋白质组学研究方法

Proteomic approaches

文献

References

呼吸跃变型果蔬 Climacteric fruits and vegetables

苹果

Malus domestica Borkh.

标记定量肽段双甲基化标记 LC/MS qTOF[21]
标记定量2-DE MALDI-TOP-MS mLC-ESI-IT-MS/MS[16]
标记定量iTRAQ nanoLC-MS/MS[22]
非标记定量非标记 LC/MS MS UPLC/MSE[23]

桃

Amygdalus persica L.

标记定量iTRAQ GC-MS[17]
标记定量2-DE MALDI-TOF/TOF MS[24]
标记定量2-DE LTQ Orbitrap XL LC-MS/MS[25]
标记定量2-DE LC-MS/MS[26]

香蕉

Musa nana Lour.

标记定量nanoLC-MS/MS GC-MS[27]
标记定量MALDI-TOF-TOF MS LC-ESI-MS/MS[28]
标记定量iTRAQ HPLC-MS ESI-QUAD-TOF[18]
标记定量2D-DIGE Q-TOP LC-MS/MS[29]

梨

Pyrus communis L.

标记定量2-DE MALDI-TOF[30]
标记定量2D-DIGE MALDI-TOF/TOF[31]
非标记定量非标记 emPAI[19]
杧果Mangifera indica L.标记定量DIGE MALDI-MS/MS[32]

杏

Prunus armeniaca L.

标记定量2-DE MALDI-TOF-PMF nanoLC-ESI-LIT-MS/MS[33]
非标记定量非标记 LC-ESI-MS/MS[34]

马铃薯

Solanum tuberosum L.

标记定量iTRAQ LC-MS/MS[5]
标记定量Nano-RPLC-MS/MS[35]

番茄

Solanum lycopersicum L.

标记定量iTRAQ NanoLC-MS/MS[36]
标记定量LC-MS和平行反应监测（PRM）[37]
标记定量2-DE MALDI-TOP/MS SDS-PAGE[38]
非呼吸跃变型果蔬 Non-climacteric fruits and vegetables

柑橘

Citrus sinensis L.

标记定量HPLC-MS/MS GS-MS[39]
标记定量2-DE MALDI-TOF-TOFMS[40]
非标记定量iTRAQ[41]
非标记定量非标记谱图计数（SC） LC-MS/MS[42]

草莓

Fragaria ananassa Duch.

标记定量iTRAQ LC-MS/MS[43]
非标记定量多重反应监测（MRM） LC-MS/MS[44]
非标记定量脱甲基肽标记 LC/MS qTOF[45]

葡萄

Vitis vinifera L.

标记定量2-DE GC-MS MALDI-TOF[46]
标记定量2-DE MALDI-TOF/TOF[47]
标记定量2-DE nano-HPLC-Chip MS/MS[48]
花椒Capsicum annuum L.标记定量2-DE DIGE MALDI-TOF/TOF[49]
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图1  果蔬采后定量蛋白质组学研究策略图
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