Please wait a minute...
浙江大学学报（农业与生命科学版）
浙江大学学报（农业与生命科学版）  2019, Vol. 45 Issue (4): 472-483    DOI: 10.3785/j.issn.1008-9209.2018.09.171
动物科学与动物医学     
BmDaxx 对家蚕细胞和幼虫组织凋亡的调控作用(英文)
矫鹏，肖颖，刘震，陈瑞婷，陆喦，辛虎虎，缪云根*
浙江大学动物科学学院蚕蜂研究所，杭州 310058
BmDaxx regulates apoptosis in cells and larval tissues of the silkworm ( Bombyx mori )
Peng JIAO(),Ying XIAO,Zhen LIU,Ruiting CHEN,Yan LU,Huhu XIN,Yungen MIAO
Institute of Sericulture and Apiculture, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China
 全文: PDF(1508 KB)   HTML
摘要：

采用荧光定量聚合酶链式反应（real-time quantitative polymerase chain reaction, RT-qPCR）方法体外扩增并鉴定家蚕死亡结构域相关蛋白基因（BmDaxx）。结果表明：BmDaxx及其下游基因BmFadd和BmDredd的表达水平在BmDaxx过表达后显著升高，但在BmDaxx的RNA干扰后，BmDaxx和BmFadd的表达水平显著降低；BmDaxx过表达增加半胱氨酸天冬氨酸蛋白酶-3/7（cystein-asparate protease-3/7, caspase-3/7）活性，而RNA干扰则降低caspase-3/7活性。用caspase-3/7抑制剂处理BmN细胞后，与对照组相比，BmDaxx、BmFadd和BmDredd的转录水平降低。在家蚕吐丝期，BmDaxx在丝腺中的表达量显著高于其他组织，这与丝腺的凋亡退化过程相一致。注射caspase抑制剂后，凋亡相关基因BmDaxx、BmFadd和BmDredd的表达水平显著降低。由此可以认为，BmDaxx及其下游基因BmFadd和BmDredd之间存在着相互作用，它们在BmN细胞和丝腺细胞凋亡中起着关键作用。
Abstract:

We amplified and characterized the BmDaxx gene of the silkworm (Bombyx mori) by real-time quantitative polymerase chain reaction (RT-qPCR). The expression levels of BmDaxx and its downstream genes BmFadd and BmDredd showed significant increases after overexpression of BmDaxx. However, the expression levels of BmDaxx and BmFadd decreased significantly after siRNA interference of BmDaxx. Overexpression of BmDaxx increased the activity of the cystein-asparate protease-3/7 (caspase-3/7), while siRNA interference decreased the activity. When BmN cells were treated by caspase-3/7 inhibitor, the transcriptional levels of BmDaxx, BmFadd and BmDredd decreased as compared with controls. The expression level of BmDaxx was significantly higher in silk glands than in other organs at the silking stage, consistent with the silk gland degeneration at this stage. After injecting of the caspase inhibitor, the expression levels of the apoptosis-related genes BmDaxx, BmFadd and BmDredd showed significant decreases. It is concluded that there are direct relationships between BmDaxx and its predicted downstream genes BmFadd and BmDredd, which play critical roles both in BmN cells and in silk gland apoptosis.
Key words: silkworm (Bombyx mori)    apoptosis    silk gland    death domain-associated protein gene in Bombyx mori (BmDaxx)    cystein-asparate protease
出版日期: 2019-09-17
CLC:  S 881.2  
服务　　
把本文推荐给朋友
加入引用管理器
E-mail Alert
RSS
作者相关文章　　
矫鹏
肖颖
刘震
陈瑞婷
陆喦
辛虎虎
缪云根

引用本文:

矫鹏, 肖颖, 刘震, 陈瑞婷, 陆喦, 辛虎虎, 缪云根. BmDaxx 对家蚕细胞和幼虫组织凋亡的调控作用(英文)[J]. 浙江大学学报（农业与生命科学版）, 2019, 45(4): 472-483.

Peng JIAO, Ying XIAO, Zhen LIU, Ruiting CHEN, Yan LU, Huhu XIN, Yungen MIAO . BmDaxx regulates apoptosis in cells and larval tissues of the silkworm ( Bombyx mori ). Journal of Zhejiang University (Agriculture and Life Sciences), 2019, 45(4): 472-483.

链接本文:

http://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2018.09.171    或    http://www.zjujournals.com/agr/CN/Y2019/V45/I4/472

  
  
  
  
  
  
  
  
1 RAFF M C . Social controls on cell survival and cell death. Nature, 1992,356(6368):397-400.
2 LOCKSHIN R A , ZAKERI Z . Apoptosis, autophagy, and more. International Journal of Biochemistry and Cell Biology, 2004,36(12):2405-2419.
3 IGNEY F H , KRAMMER P H . Death and anti-death: tumour resistance to apoptosis. Nature Reviews Cancer, 2002,2(4):277-288.
4 ELMORE S . Apoptosis: a review of programmed cell death. Toxicologic Pathology, 2007,35(4):495-516.
5 THOMPSON C B . Apoptosis in the pathogenesis and treatment of disease. Science, 1995,267(5203):1456-1462.
7 GREE D R , EVAN G I . A matter of life and death. Cancer Cell, 2002,1(1):19-30.
8 HANAHAN D , WEINBERG R A . The hallmarks of cancer. Cell, 2000,100(1):57-70.
9 VAUX D L , FLAVELL R A . Apoptosis genes and autoimmunity. Current Opinion in Immunology, 2000,12(6):719-724.
10 YUAN J , YANKNER B A . Apoptosis in the nervous system. Nature, 2000,407(6805):802-809.
11 ARAGANE Y , KULMS D , METZE D , et al . Ultraviolet light induces apoptosis via direct activation of CD95 (Fas/APO-1) independently of its ligand CD95L. Journal of Cell Biology, 1998,140(1):171-182.
12 KLEEFF J , KORNMANN M , SAWHNEY H , et al . Actinomycin D induces apoptosis and inhibits growth of pancreatic cancer cells. International Journal of Cancer, 2000,86(3):399-407.
13 YI H S , PAN C X , PAN C , et al . BmICE-2 is a novel pro-apoptotic caspase involved in apoptosis in the silkworm, Bombyx mori . Biochemical and Biophysical Research Communications, 2014,445(1):100-106.
14 CHENG J Q , ZHOU T Y , LIU C Z , et al . Protection from Fas-mediated apoptosis by a soluble form of the Fas molecule. Science, 1994,263(5154):1759-1762.
15 PERLMAN R , SCHIEMANN W P , BROOKS M W , et al . TGF-β-induced apoptosis is mediated by the adapter protein Daxx that facilitates JNK activation. Nature Cell Biology, 2001,3(8):708-714.
16 YANG X L , KHOSRAVI-FAR R , CHANG H Y , et al . Daxx, a novel Fas-binding protein that activates JNK and apoptosis. Cell, 1997,89(7):1067-1076.
17 SALOMONI P , KHELIFI A F . Daxx: death or survival protein? Trends in Cell Biology , 2006,16(2):97-104.
18 LI J S , GU L B , ZHANG H L , et al . Berberine represses DAXX gene transcription and induces cancer cell apoptosis. Laboratory Investigation, 2013,93(3):354-364.
19 SCHREINER S , WODRICH H . Virion factors that target Daxx to overcome intrinsic immunity. Journal of Virology, 2013,87(19):10412-10422.
20 FRIDMAN J S , LOWE S W . Control of apoptosis by p53. Oncogene, 2003,22(56):9030-9040.
21 MIHARA M , ERSTER S , ZAIKA A , et al . p53 has a direct apoptogenic role at the mitochondria. Molecular Cell, 2003,11(3):577-590.
22 VOUSDEN K H , LU X . Live or let die: the cell’s response to p53. Nature Reviews Cancer, 2002,2(8):594-604.
23 ZHAO L Y , LIU J L , SIDHU G S , et al . Negative regulation of p53 functions by Daxx and the involvement of MDM2. Journal of Biological Chemistry, 2004,279(48):50566-50579.
24 PARK J , LEE J H, LA M, et al . Inhibition of NF-kappaB acetylation and its transcriptional activity by Daxx. Journal of Molecular Biology, 2007,368(2):388-397.
25 XIA Q Y , ZHOU Z Y , LU C , et al . A draft sequence for the genome of the domesticated silkworm (Bombyx mori). Science, 2005,306(5703):1937-1940.
26 ZHANG X L , GU L B , LI J S , et al . Degradation of MDM2 by the interaction between berberine and DAXX leads to potent apoptosis in MDM2-overexpressing cancer cells. Cancer Research, 2010,70(23):9895-9904.
27 WANG L , SONG J , BAO X Y , et al . BmDredd is an initiator caspase and participates in emodin-induced apoptosis in the silkworm, Bombyx mori . Gene, 2016,591(2):362-368.
28 LEE D, LONG S A , MURRAY J H , et al . Potent and selective nonpeptide inhibitors of caspases 3 and 7. Journal of Medicinal Chemistry, 2001,44(12):2015-2026.
29 BOEHERE S J , NOWAK D , HOCHMUTH S . Daxx overexpression in T-lymphoblastic Jurkat cells enhances caspase-dependent death receptor- and drug-induced apoptosis in distinct ways. Cell Signal, 2005,17(5):581-595.
30 YUN H J , YOON J H , LEE J K, et al . Daxx mediates activation-induced cell death in microglia by triggering MST1 signalling. The EMBO Journal, 2011,30(12):2465-2476.
31 CHEN L Y , CHEN J D . Daxx silencing sensitizes cells to multiple apoptotic pathways. Molecular and Cellular Biology, 2003,23(20):7108-7121.
32 ZHANG J Y , PAN M H , SUN Z Y , et al . The genomic underpinnings of apoptosis in the silkworm, Bombyx mori . BMC Genomics, 2010,11(1):484-492.
33 ZOBALOVA R , COWIE E , CHLADOVA J , et al . Daxx inhibits stress-induced apoptosis in cardiac myocytes. Redox Report, 2008,13(6):263-270.
34 CHEN R T , JIAO P , LU Y , et al . BmDredd regulates the apoptosis coordinating with BmDaxx, BmCide-b, BmFadd and BmCreb in BmN cells. Archives of Insect Biochemistry and Physiology, 2016,93(3):160-173.
35 NAGARAJU J , GOLDSMITH M R . Silkworm genomics: progress and prospects. Current Science, 2002,83(4):415-425.
36 TANG J , LI W F , ZHANG X , et al . The gene expression profile of Bombyx mori silkgland. Gene, 2007,96(2):369-372.
[1] 陈艳花,蒋涛,王雪珍,钱平,唐顺明,沈兴家. 家蚕bmo-miR-0031-3p体内下调丝素轻链基因BmFib-L的表达[J]. 浙江大学学报（农业与生命科学版）, 2019, 45(2): 229-236.