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ZJUS-B
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology)  2014, Vol. 15 Issue (12): 1039-1047    DOI: 10.1631/jzus.B1400104
Articles     
Therapeutic detoxification of quercetin against carbon tetrachloride-induced acute liver injury in mice and its mechanism
Jia-qi Zhang, Liang Shi, Xi-ning Xu, Si-chong Huang, Bin Lu, Li-li Ji, Zheng-tao Wang
MOE Key Laboratory for Standardization of Chinese Medicines and Shanghai Key Laboratory of Complex Prescription, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China; School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
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Abstract  This study observes the therapeutic detoxification of quercetin, a well-known flavonoid, against carbon tetrachloride (CCl4) induced acute liver injury in vivo and explores its mechanism. Quercetin decreased CCl4-increased serum activities of alanine and aspartate aminotransferases (ALT/AST) when orally taken 30 min after CCl4 intoxication. The results of a histological evaluation further evidenced the ability of quercetin to protect against CCl4-induced liver injury. Quercetin decreased the CCl4-increased malondialdehyde (MDA) and reduced the glutathione (GSH) amounts in the liver. It also reduced the enhanced immunohistochemical staining of the 4-hydroxynonenal (4-HNE) in the liver induced by CCl4. Peroxiredoxin (Prx) 1, 2, 3, 5, 6, thioredoxin reductase 1 and 2 (TrxR1/2), thioredoxin 1 and 2 (Trx1/2), nuclear factor erythroid 2-related factor 2 (Nrf2), and heme oxygenase-1 (HO-1) all play critical roles in maintaining cellular redox homeostasis. Real-time polymerase chain reaction (PCR) results demonstrated that quercetin reversed the decreased mRNA expression of all those genes induced by CCl4. In conclusion, our results demonstrate that quercetin ameliorates CCl4-induced acute liver injury in vivo via alleviating oxidative stress injuries when orally taken after CCl4 intoxication. This protection may be caused by the elevation of the antioxidant capacity induced by quercetin.

Key words： Hepatotoxicity      Oxidative stress      Peroxiredoxin (Prx)      Nuclear factor erythroid 2-related factor 2 (Nrf2)      TrxR      Trx      HO-1     
Received: 14 April 2014      Published: 03 December 2014
CLC:  R96  
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Jia-qi Zhang
Liang Shi
Xi-ning Xu
Si-chong Huang
Bin Lu
Li-li Ji
Zheng-tao Wang
Cite this article:

Jia-qi Zhang, Liang Shi, Xi-ning Xu, Si-chong Huang, Bin Lu, Li-li Ji, Zheng-tao Wang. Therapeutic detoxification of quercetin against carbon tetrachloride-induced acute liver injury in mice and its mechanism. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2014, 15(12): 1039-1047.

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http://www.zjujournals.com/xueshu/zjus-b/10.1631/jzus.B1400104     OR     http://www.zjujournals.com/xueshu/zjus-b/Y2014/V15/I12/1039

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