Please wait a minute...
ZJUS-B
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology)  2014, Vol. 15 Issue (4): 343-352    DOI: 10.1631/jzus.B1300246
Articles     
Enhanced production of thermostable laccases from a native strain of Pycnoporus sanguineus using central composite design
Leticia I. Ramírez-Cavazos, Charles Junghanns, Rakesh Nair, Diana L. Cárdenas-Chávez, Carlos Hernández-Luna, Spiros N. Agathos, Roberto Parra
Centro del Agua para América Latina y el Caribe, Tecnológico de Monterrey, Campus Monterrey, NL 64849, Mexico; Helmholtz Centre for Environmental Research-UFZ, Permoserstra?e 15, 04318 Leipzig, Germany; Bioengineering Group, Earth and Life Institute, Université Catholique de Louvain, Place Croix du Sud, 2, Box L7.05.19, 1348 Louvain-la-Neuve, Belgium; Laboratorio de Enzimología, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, Av. Universidad s/n, Ciudad Universitaria, San Nicolás de los Garza, NL 64450, Mexico
Download:     PDF (0 KB)     
Export: BibTeX | EndNote (RIS)      

Abstract  The production of thermostable laccases from a native strain of the white-rot fungus Pycnoporus sanguineus isolated in Mexico was enhanced by testing different media and a combination of inducers including copper sulfate (CuSO4). The best conditions obtained from screening experiments in shaken flasks using tomato juice, CuSO4, and soybean oil were integrated in an experimental design. Enhanced levels of tomato juice as the medium, CuSO4 and soybean oil as inducers (36.8% (v/v), 3 mmol/L, and 1% (v/v), respectively) were determined for 10 L stirred tank bioreactor runs. This combination resulted in laccase titer of 143000 IU/L (2,2\'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid), pH 3.0), which represents the highest activity so far reported for P. sanguineus in a 10-L fermentor. Other interesting media resulting from the screening included glucose-bactopeptone which increased laccase activity up to 20000 IU/L, whereas the inducers Acid Blue 62 and Reactive Blue 19 enhanced enzyme production in this medium 10 times. Based on a partial characterization, the laccases of this strain are especially promising in terms of thermostability (half-life of 6.1 h at 60 °C) and activity titers.

Key words： Enhanced laccase production      Central composite design      Screening media      Inducers      Tomato juice medium      Soybean oil      Copper sulfate     
Received: 12 September 2013      Published: 06 April 2014
CLC:  Q936  
Service
E-mail this article
Add to my bookshelf
Add to citation manager
E-mail Alert
RSS
Articles by authors
Leticia I. Ramírez-Cavazos
Diana L. Cárdenas-Chávez
Charles Junghanns
Rakesh Nair
Carlos Hernández-Luna
Spiros N. Agathos
Roberto Parra
Cite this article:

Leticia I. Ramírez-Cavazos, Charles Junghanns, Rakesh Nair, Diana L. Cárdenas-Chávez, Carlos Hernández-Luna, Spiros N. Agathos, Roberto Parra. Enhanced production of thermostable laccases from a native strain of Pycnoporus sanguineus using central composite design. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2014, 15(4): 343-352.

URL:

http://www.zjujournals.com/xueshu/zjus-b/10.1631/jzus.B1300246     OR     http://www.zjujournals.com/xueshu/zjus-b/Y2014/V15/I4/343

[1] Shu-fang Wang, Hai-yan Fang, Hai-bin Qu. Optimization of micellar electrokinetic capillary chromatography method using central composite design for the analysis of components in Yangwei granule[J]. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2011, 12(3): 193-200.
[2] Yao-xing XU, Yan-li LI, Shao-chun XU, Yong LIU, Xin WANG, Jiang-wu TANG. Improvement of xylanase production by Aspergillus niger XY-1 using response surface methodology for optimizing the medium composition[J]. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2008, 9(7): 558-566.