Please wait a minute...
ZJUS-B
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology)  2008, Vol. 9 Issue (7): 558-566    DOI: 10.1631/jzus.B0820038
Biotechnology and Biochemistry     
Improvement of xylanase production by Aspergillus niger XY-1 using response surface methodology for optimizing the medium composition
Yao-xing XU, Yan-li LI, Shao-chun XU, Yong LIU, Xin WANG, Jiang-wu TANG
Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
Download:     PDF (0 KB)     
Export: BibTeX | EndNote (RIS)      

Abstract  Objective: To study the optimal medium composition for xylanase production by Aspergillus niger XY-1 in solid-state fermentation (SSF). Methods: Statistical methodology including the Plackett-Burman design (PBD) and the central composite design (CCD) was employed to investigate the individual crucial component of the medium that significantly affected the enzyme yield. Results: Firstly, NaNO3, yeast extract, urea, Na2CO3, MgSO4, peptone and (NH4)2SO4 were screened as the significant factors positively affecting the xylanase production by PBD. Secondly, by valuating the nitrogen sources effect, urea was proved to be the most effective and economic nitrogen source for xylanase production and used for further optimization. Finally, the CCD and response surface methodology (RSM) were applied to determine the optimal concentration of each significant variable, which included urea, Na2CO3 and MgSO4. Subsequently a second-order polynomial was determined by multiple regression analysis. The optimum values of the critical components for maximum xylanase production were obtained as follows: x1 (urea)=0.163 (41.63 g/L), x2 (Na2CO3)=−1.68 (2.64 g/L), x3 (MgSO4)=1.338 (10.68 g/L) and the predicted xylanase value was 14374.6 U/g dry substrate. Using the optimized condition, xylanase production by Aspergillus niger XY-1 after 48 h fermentation reached 14637 U/g dry substrate with wheat bran in the shake flask. Conclusion: By using PBD and CCD, we obtained the optimal composition for xylanase production by Aspergillus niger XY-1 in SSF, and the results of no additional expensive medium and shortened fermentation time for higher xylanase production show the potential for industrial utilization.

Key words： Xylanase      Optimization of medium composition      Plackett-Burman design (PBD)      Central composite design (CCD)      Response surface methodology (RSM)      Aspergillus niger XY-1     
Received: 29 January 2008     
CLC:  Q55  
Service
E-mail this article
Add to my bookshelf
Add to citation manager
E-mail Alert
RSS
Articles by authors
Yao-xing XU
Yan-li LI
Shao-chun XU
Yong LIU
Xin WANG
Jiang-wu TANG
Cite this article:

Yao-xing XU, Yan-li LI, Shao-chun XU, Yong LIU, Xin WANG, Jiang-wu TANG. Improvement of xylanase production by Aspergillus niger XY-1 using response surface methodology for optimizing the medium composition. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2008, 9(7): 558-566.

URL:

http://www.zjujournals.com/xueshu/zjus-b/10.1631/jzus.B0820038     OR     http://www.zjujournals.com/xueshu/zjus-b/Y2008/V9/I7/558

[1] Xiao-xin Chen, Xiao-bing Wu, Wei-ming Chai, Hui-ling Feng, Yan Shi, Han-tao Zhou, Qing-xi Chen. Optimization of extraction of phenolics from leaves of Ficus virens[J]. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2013, 14(10): 903-915.
[2] Yun-jian Zhang, Qiang Li, Yu-xiu Zhang, Dan Wang, Jian-min Xing. Optimization of succinic acid fermentation with Actinobacillus succinogenes by response surface methodology (RSM)[J]. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2012, 13(2): 103-110.
[3] Yong-liang ZHUANG, Xue ZHAO, Ba-fang LI. Optimization of antioxidant activity by response surface methodology in hydrolysates of jellyfish (Rhopilema esculentum) umbrella collagen[J]. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2009, 10(8): 572-579.
[4] XU Ying, HE Guo-qing, LI Jing-jun. Effective extraction of elastase from Bacillus sp. fermentation broth using aqueous two-phase system[J]. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2005, 6(11): 7-.