Please wait a minute...
ZJUS-B
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology)  2005, Vol. 6 Issue (11): 7-    DOI: 10.1631/jzus.2005.B1087
    
Effective extraction of elastase from Bacillus sp. fermentation broth using aqueous two-phase system
XU Ying, HE Guo-qing, LI Jing-jun
Department of Food Science and Nutrition, Zhejiang University, Hangzhou 310029, China; School of Food Science and Engineering, Ocean University of China, Qingdao 266003, China; School of Food Science and Technology, Southern Yangtze University, Wuxi 214036, China
Download:     PDF (0 KB)     
Export: BibTeX | EndNote (RIS)      

Abstract  This paper presents the evaluation of an aqueous two-phase system (ATPS) for extracting elastase produced by Bacillus sp. EL31410. The elastase and cell partition behavior in polyethylene glycol (PEG)/salt systems was investigated. The suitable system for elastase extraction was PEG/KH2PO4-K2HPO4, in which elastase is mainly partitioned into the PEG-rich phase, while the cells remained in the other phase. The influence of defined system parameters (e.g. PEG molecular mass, pH, NaCl addition) on the partitioning behavior of elastase is described. The concentration of phase forming components, PEG and KH2PO4-K2HPO4, was optimized for elastase recovery by means of response surface methodology, and it was found that they greatly influenced extraction recovery. The optimal ATPS was 23.1% (w/w) PEG 2 000 and 11.7% (w/w) KH2PO4-K2HPO4. The predicted recovery was about 89.5%, so this process is suggested to be a rapid and convenient method for elastase extraction.

Key words： Elastase      Aqueous two-phase system (ATPS)      Bioseparation      Purification      Optimization      Response surface methodology (RSM)     
Received: 07 June 2005     
CLC:  TQ925  
Service
E-mail this article
Add to my bookshelf
Add to citation manager
E-mail Alert
RSS
Articles by authors
XU Ying
HE Guo-qing
LI Jing-jun
Cite this article:

XU Ying, HE Guo-qing, LI Jing-jun. Effective extraction of elastase from Bacillus sp. fermentation broth using aqueous two-phase system. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2005, 6(11): 7-.

URL:

http://www.zjujournals.com/xueshu/zjus-b/10.1631/jzus.2005.B1087     OR     http://www.zjujournals.com/xueshu/zjus-b/Y2005/V6/I11/7

[1] Ya-qian Li, Kai Song, Ya-chai Li, Jie Chen. Statistical culture-based strategies to enhance chlamydospore production by Trichoderma harzianum SH2303 in liquid fermentation[J]. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2016, 17(8): 619-627.
[2] Qing Zhang, Hui Yu, Feng-zhen Zhang, Zhi-cheng Shen. Expression and purification of recombinant human serum albumin from selectively terminable transgenic rice[J]. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2013, 14(10): 867-874.
[3] Xiao-xin Chen, Xiao-bing Wu, Wei-ming Chai, Hui-ling Feng, Yan Shi, Han-tao Zhou, Qing-xi Chen. Optimization of extraction of phenolics from leaves of Ficus virens[J]. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2013, 14(10): 903-915.
[4] Yan Xing, Hong-yun Peng, Meng-xi Zhang, Xia Li, Wei-wei Zeng, Xiao-e Yang. Caffeic acid product from the highly copper-tolerant plant Elsholtzia splendens post-phytoremediation: its extraction, purification, and identification[J]. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2012, 13(6): 487-493.
[5] Yun-jian Zhang, Qiang Li, Yu-xiu Zhang, Dan Wang, Jian-min Xing. Optimization of succinic acid fermentation with Actinobacillus succinogenes by response surface methodology (RSM)[J]. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2012, 13(2): 103-110.
[6] Mei-ling Chen, Qin Guo, Rui-zhi Wang, Juan Xu, Chen-wei Zhou, Hui Ruan, Guo-qing He. Construction of the yeast whole-cell Rhizopus oryzae lipase biocatalyst with high activity[J]. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2011, 12(7): 545-551.
[7] Xiu-ju Du, Jing-song Zhang, Yan Yang, Qing-jiu Tang, Wei Jia, Ying-jie Pan. Purification, chemical modification and immunostimulating activity of polysaccharides from Tremella aurantialba fruit bodies[J]. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2010, 11(6): 437-442.
[8] Yong-liang ZHUANG, Xue ZHAO, Ba-fang LI. Optimization of antioxidant activity by response surface methodology in hydrolysates of jellyfish (Rhopilema esculentum) umbrella collagen[J]. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2009, 10(8): 572-579.
[9] Jing LI, Qian YANG, Li-hua ZHAO, Shu-mei ZHANG, Yu-xia WANG, Xiao-yu ZHAO. Purification and characterization of a novel antifungal protein from Bacillus subtilis strain B29[J]. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2009, 10(4): 264-272.
[10] Zhong-hui ZHANG, Li-juan DU, Di XIANG, Shun-ying ZHU, Ming-yuan WU, Hui-li LU, Yan YU, Wei HAN. Expression and purification of bioactive high-purity human midkine in Escherichia coli[J]. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2009, 10(2): 79-86.
[11] Ming LIU, Jun YANG, Yun-qing CHENG, Li-jia AN. Optimization of soybean (Glycine max (L.) Merrill) in planta ovary transformation using a linear minimal gus gene cassette[J]. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2009, 10(12): 870-876.
[12] Cheng-gang CAI, Ji-shuang CHEN, Jiong-jiong QI, Yun YIN, Xiao-dong ZHENG. Purification and characterization of keratinase from a new Bacillus subtilis strain[J]. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2008, 9(9): 713-720.
[13] Yao-xing XU, Yan-li LI, Shao-chun XU, Yong LIU, Xin WANG, Jiang-wu TANG. Improvement of xylanase production by Aspergillus niger XY-1 using response surface methodology for optimizing the medium composition[J]. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2008, 9(7): 558-566.
[14] Hui NI, Qi-he CHEN, Guo-qing HE, Guang-bin WU, Yuan-fan YANG. Optimization of acidic extraction of astaxanthin from Phaffia rhodozyma[J]. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2008, 9(1): 51-59.
[15] NI Hui, CHEN Qi-he, RUAN Hui, YANG Yuan-fan, LI Li-jun, WU Guang-bin, HU Yang, HE Guo-qing. Studies on optimization of nitrogen sources for astaxanthin production by Phaffia rhodozyma[J]. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2007, 8(5): 365-370.