| Biotechnology |
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| Purification, chemical modification and immunostimulating activity of polysaccharides from Tremella aurantialba fruit bodies |
| Xiu-ju Du, Jing-song Zhang, Yan Yang, Qing-jiu Tang, Wei Jia, Ying-jie Pan |
| Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China, College of Life Science, Liaocheng University, Liaocheng 252059, China, College of Food Science, Shanghai Ocean University, Shanghai 200090, China |
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Abstract Ultrafiltration and a series of chromatographic steps were used to isolate and purify polysaccharides from Tremella aurantialba fruit bodies. Three crude fractions (TAP50w, TAP10–50w, and TAP1–10w), five semi-purified fractions (TAPA–TAPE), and one purified fraction (TAPA1) were obtained. A sulfated derivative of TAPA1 (TAPA1-s) was prepared by chemical modification. The immunostimulating activity of the polysaccharide fractions in vitro was determined using the mouse spleen lymphocyte proliferation assay. Of the three crude fractions tested, cell proliferation rates were increased most by TAP50w. Furthermore, TAPA1-s was markedly more stimulatory than TAPA1, indicating that sulfonation was an effective way to enhance the immunostimulating activity of polysaccharide.
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Received: 11 December 2009
Published: 02 June 2010
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