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ZJUS-B
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology)  2012, Vol. 13 Issue (6): 487-493    DOI: 10.1631/jzus.B1100298
Articles     
Caffeic acid product from the highly copper-tolerant plant Elsholtzia splendens post-phytoremediation: its extraction, purification, and identification
Yan Xing, Hong-yun Peng, Meng-xi Zhang, Xia Li, Wei-wei Zeng, Xiao-e Yang
Ministry of Education Key Laboratory of Environmental Remediation and Ecological Health, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, China; State Key Laboratory of Hydrology-Water Resources and Hydraulic Engineering, Hohai University, Nanjing 210098, China
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Abstract  In the current study, caffeic acid was an important metabolite in the highly copper-tolerant plant Elsholtzia splendens. Preparation and purification of caffeic acid were performed on the dried biomass of the plants by means of sonication/ethanol extraction, followed by purification using a macroporous resin (D101 type) column and silica gel chromatography. The faint-yellow caffeic acid product was yielded with a purity of 98.46%, and it was chemically identified from spectra of Fourier transform infrared spectroscopy (FTIR), proton nuclear magnetic resonance (1H NMR)/carbon nuclear magnetic resonance (13C NMR), and electrospray ionization mass spectrometry (ESI-MS). Caffeic acid is a possible product from the post-harvest processing of Elsholtzia splendens biomass.

Key words： Caffeic acid      Purification      Post-processing      Copper tolerance      Elsholtzia splendens     
Received: 16 September 2011      Published: 06 June 2012
CLC:  X53  
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Yan Xing
Hong-yun Peng
Meng-xi Zhang
Xia Li
Wei-wei Zeng
Xiao-e Yang
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Yan Xing, Hong-yun Peng, Meng-xi Zhang, Xia Li, Wei-wei Zeng, Xiao-e Yang. Caffeic acid product from the highly copper-tolerant plant Elsholtzia splendens post-phytoremediation: its extraction, purification, and identification. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2012, 13(6): 487-493.

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http://www.zjujournals.com/xueshu/zjus-b/10.1631/jzus.B1100298     OR     http://www.zjujournals.com/xueshu/zjus-b/Y2012/V13/I6/487

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