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ZJUS-B
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology)  2013, Vol. 14 Issue (4): 346-354    DOI: 10.1631/jzus.B1200153
Articles     
Enhancing production of a 24-membered ring macrolide compound by a marine bacterium using response surface methodology
Hua Chen, Mian-bin Wu, Zheng-jie Chen, Ming-lu Wang, Jian-ping Lin, Li-rong Yang
Key Laboratory of Biomass Chemical Engineering of Ministry of Education, Department of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, China; Zhejiang Key Laboratory of Antifungal Drugs, Taizhou 318000, China
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Abstract  A 24-membered ring macrolide compound, macrolactin A has potential applications in pharmaceuticals for its anti-infectious and antiviral activity. In this study, macrolactin A was produced by a marine bacterium, which was identified as Bacillus subtilis by 16S ribosomal RNA (rRNA) sequence analysis. Electrospray ionization mass spectrometry (ESI/MS) and nuclear magnetic resonance (NMR) spectroscopy analyses were used to characterize this compound. To improve the production, response surface methodology (RSM) involving Box-Behnken design (BBD) was employed. Faeces bombycis, the main by-product in sericulture, was used as a nitrogen source in fermentation. The interactions between three significant factors, F. bombycis, soluble starch, and (NH4)2SO4 were investigated. A quadratic model was constructed to fit the production and the factors. Optimum medium composition was obtained by analysis of the model. When cultivated in the optimum medium, the production of macrolactin A was increased to 851 mg/L, 2.7 times as compared to the original. This study is also useful to find another way in utilizing F. bombycis.

Key words： 24-membered ring macrolide      Enhancing production      Response surface methodology      Faeces bombycis      Marine bacterium     
Received: 30 May 2012      Published: 03 April 2013
CLC:  Q815  
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Hua Chen
Mian-bin Wu
Zheng-jie Chen
Ming-lu Wang
Jian-ping Lin
Li-rong Yang
Cite this article:

Hua Chen, Mian-bin Wu, Zheng-jie Chen, Ming-lu Wang, Jian-ping Lin, Li-rong Yang. Enhancing production of a 24-membered ring macrolide compound by a marine bacterium using response surface methodology. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2013, 14(4): 346-354.

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http://www.zjujournals.com/xueshu/zjus-b/10.1631/jzus.B1200153     OR     http://www.zjujournals.com/xueshu/zjus-b/Y2013/V14/I4/346

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