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ZJUS-B
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology)  2013, Vol. 14 Issue (3): 216-223    DOI: 10.1631/jzus.B1200102
Articles     
Retracted Article: Characterization of cancer stem-like cells in the side population cells of human gastric cancer cell line MKN-45
Hai-hong Zhang, Ai-zhen Cai, Xue-ming Wei, Li Ding, Feng-zhi Li, Ai-ming Zheng, Da-jiang Dai, Rong-rong Huang, Hou-jun Cao, Hai-yang Zhou, Jian-mei Wang, Xue-jing Wang, Wei Shi, Heng Zhu, Xiao-ying Yuan, Lin Chen
Department of General Surgery, General Hospital of the Air Force, PLA, Beijing 100142, China; Department of General Surgery, Chinese PLA General Hospital, Beijing 100853, China; Department of Cell Biology, Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing 100850, China
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Abstract  Objective: Side population (SP) cells may play a crucial role in tumorigenesis and the recurrence of cancer. Many kinds of cell lines and tissues have demonstrated the presence of SP cells, including several gastric cancer cell lines. This study is aimed to identify the cancer stem-like cells in the SP of gastric cancer cell line MKN-45. Methods: We used fluorescence activated cell sorting (FACS) to sort SP cells in the human gastric carcinoma cell line MKN-45 (cells labeled with Hoechst 33342) and then characterized the cancer stem-like properties of SP cells. Results: This study found that the SP cells had higher clone formation efficiency than major population (MP) cells. Five stemness-related gene expression profiles, including OCT-4, SOX-2, NANOG, CD44, and adenosine triphosphate (ATP)-binding cassette transporters gene ABCG2, were tested in SP and MP cells using quantitative real-time reverse transcription polymerase chain reaction (RT-PCR). Western blot was used to show the difference of protein expression between SP and MP cells. Both results show that there was significantly higher protein expression in SP cells than in MP cells. When inoculated into non-obese diabetic/severe combined immunodeficiency (NOD/SCID) mice, SP cells show higher tumorigenesis tendency than MP cells. Conclusions: These results indicate that SP cells possess cancer stem cell properties and prove that SP cells from MKN-45 are gastric cancer stem-like cells.

Key words： ATP-binding cassette transporters      Side population cells      Stem cells      Benzimidazole (Hoechst 33342)      10.1631/jzus.B12r0102

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Retraction Note of this article can be found at doi:10.1631/jzus.B12r0102

     
Received: 01 April 2012      Published: 06 March 2013
CLC:  R735  
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Articles by authors
Hai-hong Zhang
Hou-jun Cao
Hai-yang Zhou
Jian-mei Wang
Xue-jing Wang
Wei Shi
Heng Zhu
Xiao-ying Yuan
Lin Chen
Xue-ming Wei
Ai-zhen Cai
Li Ding
Feng-zhi Li
Ai-ming Zheng
Da-jiang Dai
Rong-rong Huang
Cite this article:

Hai-hong Zhang, Ai-zhen Cai, Xue-ming Wei, Li Ding, Feng-zhi Li, Ai-ming Zheng, Da-jiang Dai, Rong-rong Huang, Hou-jun Cao, Hai-yang Zhou, Jian-mei Wang, Xue-jing Wang, Wei Shi, Heng Zhu, Xiao-ying Yuan, Lin Chen. Retracted Article: Characterization of cancer stem-like cells in the side population cells of human gastric cancer cell line MKN-45. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2013, 14(3): 216-223.

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http://www.zjujournals.com/xueshu/zjus-b/10.1631/jzus.B1200102     OR     http://www.zjujournals.com/xueshu/zjus-b/Y2013/V14/I3/216

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