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| Preliminary study on the freeze-drying of human bone marrow-derived mesenchymal stem cells |
| Shao-zhi Zhang, Huan Qian, Zhen Wang, Ju-li Fan, Qian Zhou, Guang-ming Chen, Rui Li, Shan Fu, Jie Sun |
| Refrigeration and Cryogenic Engineering Institute, Zhejiang University, Hangzhou 310027, China, Bone Marrow Transplantation Center, the First Affiliate Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China |
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Abstract Long-term preservation and easy transportation of human bone marrow-derived mesenchymal stem cells (hBM-MSCs) will facilitate their application in medical treatment and bioengineering. A pilot study on the freeze-drying of hBM-MSCs was carried out. hBM-MSCs were loaded with trehalose. The glass transition temperature of the freeze-drying suspension was measured to provide information for the cooling and primary drying experiment. After freeze-drying, various rehydration processes were tested. The highest recovery rate of hBM-MSCs was (69.33卤13.08)%. Possible methods to improve freeze-drying outcomes are discussed. In conclusion, the present study has laid a foundation for the freeze-drying hBM-MSCs.
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Received: 19 May 2010
Published: 04 November 2010
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Cite this article:
Shao-zhi Zhang, Huan Qian, Zhen Wang, Ju-li Fan, Qian Zhou, Guang-ming Chen, Rui Li, Shan Fu, Jie Sun. Preliminary study on the freeze-drying of human bone marrow-derived mesenchymal stem cells. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2010, 11(11): 889-894.
URL:
http://www.zjujournals.com/xueshu/zjus-b/10.1631/jzus.B1000184 OR http://www.zjujournals.com/xueshu/zjus-b/Y2010/V11/I11/889
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