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ZJUS-B
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology)  2012, Vol. 13 Issue (1): 20-28    DOI: 10.1631/jzus.B1100117
Articles     
Effects of insulin-like growth factor-1 on the properties of mesenchymal stem cells in vitro
Yu-li Huang, Ruo-feng Qiu, Wei-yi Mai, Jian Kuang, Xiao-yan Cai, Yu-gang Dong, Yun-zhao Hu, Yuan-bin Song, An-ping Cai, Zhi-gao Jiang
Department of Cardiology, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, China, Department of Cardiology, the First People's Hospital of Shunde, Foshan 528300, China
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Abstract  Objective: To explore the effects of insulin-like growth factor-1 (IGF-1) on migration, proliferation and differentiation of mesenchymal stem cells (MSCs). Methods: MSCs were obtained from Sprague-Dawley rats by a combination of gradient centrifugation and cell culture techniques and treated with IGF-1 at concentrations of 5–20 ng/ml. Proliferation of MSCs was determined as the mean doubling time. Expression of CXC chemokine receptor 4 (CXCR4) and migration property were determined by flow cytometry and transwell migration essay, respectively. mRNA expression of GATA-4 and collagen II was determined by reverse transcription-polymerase chain reaction (RT-PCR). Results: The mean doubling time of MSC proliferation was decreased, and the expression of CXCR4 on MSCs and migration of MSCs were increased by IGF-1, all in a dose-dependent manner, while the optimal concentration of IGF-1 on proliferation and migration was different. IGF-1 did not affect the expression of GATA-4 or collagen II mRNA. Conclusions: IGF-1 dose-dependently stimulated the proliferation of MSCs, upregulated the expression of CXCR4, and accelerated migration. There was no apparent differentiation of MSCs to cardiomyocytes or chondrocytes after culturing with IGF-1 alone.

Key words： Mesenchymal stem cells (MSCs)      Proliferation      Differentiation      Insulin-like growth factor-1 (IGF-1)      CXC chemokine receptor 4 (CXCR4)      Migration     
Received: 09 April 2011      Published: 30 December 2011
CLC:  Q291  
  R542.2  
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Articles by authors
Yu-li Huang
An-ping Cai
Zhi-gao Jiang
Ruo-feng Qiu
Wei-yi Mai
Jian Kuang
Xiao-yan Cai
Yu-gang Dong
Yun-zhao Hu
Yuan-bin Song
Cite this article:

Yu-li Huang, Ruo-feng Qiu, Wei-yi Mai, Jian Kuang, Xiao-yan Cai, Yu-gang Dong, Yun-zhao Hu, Yuan-bin Song, An-ping Cai, Zhi-gao Jiang. Effects of insulin-like growth factor-1 on the properties of mesenchymal stem cells in vitro. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2012, 13(1): 20-28.

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http://www.zjujournals.com/xueshu/zjus-b/10.1631/jzus.B1100117     OR     http://www.zjujournals.com/xueshu/zjus-b/Y2012/V13/I1/20

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