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ZJUS-B
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology)  2012, Vol. 13 Issue (11): 884-893    DOI: 10.1631/jzus.B1200037
Articles     
ST13, a proliferation regulator, inhibits growth and migration of colorectal cancer cell lines
Rui Bai, Zhong Shi, Jia-wei Zhang, Dan Li, Yong-liang Zhu, Shu Zheng
Cancer Institute (Key Laboratory of Cancer Prevention and Intervention, China National Ministry of Education, Key Laboratory of Molecular Biology in Medical Sciences, Zhejiang Province, China), the Second Affiliated Hospital School of Medicine, Zhejiang University, Hangzhou 310009, China; Department of Gastroenterology, the Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China
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Abstract  Background and objective: ST13, is the gene encoding the HSP70 interacting protein (HIP). Previous research has shown that ST13 mRNA and protein levels are down-regulated in colorectal cancer (CRC) tissues compared with adjacent normal tissues. This study aims at the role of ST13 in the proliferation and migration of CRC cells. Methods: The transcript level of ST13 in different CRC cell lines was evaluated by quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR). ST13-overexpressed and ST13-knockdown CRC cells were constructed respectively by lentiviral transduction, followed by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) assay, plate colony formation, cell-cycle analysis, and migration assays to evaluate the influence of ST13 on proliferation and migration in vitro. Moreover, a mouse xenograft study was performed to test in vivo tumorigenicity of ST13-knockdown CRC cells. Results: Lentivirus-mediated overexpression of ST13 in CRC cells inhibited cell proliferation, colony formation, and cell migration in vitro. In contrast, down-regulation of ST13 by lentiviral-based short hairpin RNA (shRNA) interference in CRC cells significantly increased cell proliferation and cloning efficiency in vitro. In addition, down-regulation of ST13 expression significantly increased the tumorigenicity of CRC cells in vivo. Conclusions: ST13 gene is a proliferation regulator that inhibits tumor growth in CRC and may affect cell migration.

Key words： Colorectal cancer      ST13      Proliferation      Colony formation      Cell cycle      Migration     
Received: 07 February 2012      Published: 05 November 2012
CLC:  R735.3+5  
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Rui Bai
Zhong Shi
Jia-wei Zhang
Dan Li
Yong-liang Zhu
Shu Zheng
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Rui Bai, Zhong Shi, Jia-wei Zhang, Dan Li, Yong-liang Zhu, Shu Zheng. ST13, a proliferation regulator, inhibits growth and migration of colorectal cancer cell lines. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2012, 13(11): 884-893.

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http://www.zjujournals.com/xueshu/zjus-b/10.1631/jzus.B1200037     OR     http://www.zjujournals.com/xueshu/zjus-b/Y2012/V13/I11/884

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