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ZJUS-B
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology)  2010, Vol. 11 Issue (9): 719-727    DOI: 10.1631/jzus.B1000079
Zoology     
Effect of interrupted endogenous BMP/Smad signaling on growth and steroidogenesis of porcine granulosa cells
Wei Wang, Li Wang, Xin-xiu Li, Xia Chen, Hai-yan Zhang, Yu He, Jing-jing Wang, Yong-yan Zhao, Bao-le Zhang, Yin-xue Xu
College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China
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Abstract  Bone morphogenetic proteins (BMPs) play a critical role in the growth and steroidogenesis of granulosa cells (GCs). BMP signals act through membrane-bound heteromeric serine/threonine kinase receptors. Upon ligand binding, BMPs activate intracellular Smad proteins and regulate growth and apoptosis in various cell types. The objective of this study was to demonstrate the effects of BMP/Smad signal on growth and steroidogenesis of porcine GCs. A strategy of RNA interference (RNAi)-mediated ‘gene silencing’ of Smad4, a core molecule mediating the intracellular BMP/Smad signal transduction pathways, was used to interrupt endogenous BMP/Smad signaling. Results indicate that Smad4-small interfering RNA (siRNA) caused specific inhibition of Smad4 mRNA and protein expression after transfection. Interrupted endogenous BMP/Smad signaling significantly inhibited growth, and induced apoptosis of porcine GCs, while decreasing estradiol production. In addition, interrupted BMP/Smad signaling significantly (P<0.05) changed the expression of Cyclin D2, CDK4, Bcl-2, and Cyp19a1. These findings provide new insights into how BMP/Smad signaling regulates the growth and steroidogenesis of porcine GCs.

Key words： Porcine granulosa cells      Bone morphogenetic protein (BMP)/Smad      Smad4      RNA interference (RNAi)      Growth      Steroidogenesis     
Received: 22 February 2010      Published: 08 September 2010
CLC:  Q93  
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Bao-le Zhang
Yin-xue Xu
Xin-xiu Li
Xia Chen
Wei Wang
Li Wang
Hai-yan Zhang
Yu He
Jing-jing Wang
Yong-yan Zhao
Cite this article:

Wei Wang, Li Wang, Xin-xiu Li, Xia Chen, Hai-yan Zhang, Yu He, Jing-jing Wang, Yong-yan Zhao, Bao-le Zhang, Yin-xue Xu. Effect of interrupted endogenous BMP/Smad signaling on growth and steroidogenesis of porcine granulosa cells. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2010, 11(9): 719-727.

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http://www.zjujournals.com/xueshu/zjus-b/10.1631/jzus.B1000079     OR     http://www.zjujournals.com/xueshu/zjus-b/Y2010/V11/I9/719

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