Species inventory is the basis for understanding biodiversity. The classification system of lycophytes and ferns developed by ZHANG Xianchun was adopted to analyze the species diversity of lycophytes and ferns in Zhejiang province. The results showed that (1) there were 27 species of lycophytes in 3 families, 5 genera, and 410 species of ferns in 32 families, 84 genera (including subspecies of non-type species). The top 7 families with more than 15 species were Dryopteridaceae (95 species), Polypodiaceae (51 species), Athyriaceae (51 species), Pteridaceae (49 species), Thelypteridaceae (43 species), Aspleniaceae (27 species) and Dennstaedtiaceae (17 species). The top 6 genera with more than 15 species were Dryopteris (43 species), Asplenium (24 species), Diplazium (21 species), Polystichum (20 species), Pteris (18 species) and Arachniodes (16 species). (2) at genus level, 54 genera of plants distributed in tropic and subtropic regions, 15 genera of plants distributed in temperate zones, and 31 genera of plants distributed in pantropic type, accounting for respectively 60.67%, 16.85%, and 34.83% of the total genera, were recorded. (3) as to the areal types of species, 354 species were not endemic to China, accounting for 81.01% of the total species, and these species were mainly distributed in East Asia and tropical Asia. 83 species were endemic to China, accounting for 18.99% of the total species (except those with world-wide distribution). Among them, East China-Central China and South China-Southwest China (to Northwest China) were two dominant areal types, with 24 species. (4) The ancient plant data and modern species all reflected the ancient origin of the flora. The prolycopod (including 27 species in Lycopodiaceae, Isoetaceae and Selaginellaceae) can be traced back to the devonian period; the ferns in Equisetaceae, Ophioglossaceae, Psilotaceae, Marattiaceae (10 species in total) originated relatively early in comparison to ferns in other families. (5) among lycophytes and ferns in Zhejiang province, 5 species were listed as key-protected wild plants in Zhejiang province; 3 species Isoetes sinensis, Isoetes orientalis, and Isoetes baodongii were in the list of Ⅰ-class national protection of wild plants; 11 species of wild plants were in the list of Ⅱ-class national protection of wild plants, including Huperzia selago var. appressa, Huperzia sutchueniana, Cibotium barometz, etc.