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浙江大学学报(工学版)
J4  2009, Vol. 43 Issue (8): 1424-1427    DOI: 10.3785/j.issn.1008-973X.2009.
电子、通信与自动控制技术     
基于表面等离子共振的全自动浓度检测系统
赵光灿1,王晓萍1,陈惠滨1,罗昭锋2,张雪姣1
(1.浙江大学 现代光学仪器国家重点实验室, 浙江 杭州 310027； 2. 中国科学技术大学 生命科学学院,安徽 合肥 230027)
Automatic concentration detection system based on surface plasmon resonance
ZHAO Guang-can1, WANG Xiao-ping1, CHEN Hui-bin1, LUO Zhao-feng2, ZHANG Xue-jiao1
1. State Key Laboratory of Modern Optical Instrumentation, Zhejiang University, Hangzhou 310027, China;
2. College of Life Science, University of Science and Technology of China, Hefei 230027, China
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摘要：

研制了以SpreetaTM传感器为核心,结合液体处理系统、信号检测与控制器、计算机分析处理软件等模块的小型表面等离子共振（SPR）检测系统.运用该系统,通过在传感器表面偶联牛血清蛋白（BSA）,采用直接检测法测量了不同浓度的BSA抗体溶液,得到了BSA抗体溶液的SPR传感图,建立了BSA抗体的浓度标准曲线.在传感器表面固定雌二醇（E2）,采用间接检测法测量了不同浓度的E2溶液,根据E2溶液SPR响应信号的变化量,建立了E2的浓度标准曲线.实验中系统的分辨率为10-5折射率单位（RIU）.对于实际的待测样品,只要测得其SPR信号的变化量,结合标准曲线就能够得出样品的浓度.
Abstract:

Combining some modules including liquid treatment system, signal detection and control unit, computer analysis and processing software, a small surface plasmon resonance (SPR) detection system with SpreetaTM sensor as its core component was developed. By using this system, bovine serum albumin (BSA) antigens were immobilized on the sensitive layer of the SPR sensor and the BSA antibody solutions of different concentrations were detected with the direct assay method. A sensorgram of SPR signal about BSA antibody solutions was generated and a calibration curve was obtained. By immobilizing estradiols (E2) on the sensitive layer, E2 solutions of different concentrations were detected using the indirect assay method and a calibration curve was obtained according to the change of SPR signal. In these experiments, the system resolution was 10-5 refractive index unit (RIU). For the samples of unknown concentration, once the SPR signal change is detected, the concentration can be calculated from the calibration curve.
出版日期: 2009-09-28
:  TP 212.14  
基金资助:

国家“863”高技术研究发展计划资助项目（2006AA06Z406，2007AA09Z114）；浙江省科技计划重大资助项目（2006C12102）
通讯作者: 王晓萍，女，教授.     E-mail: xpwang@zju.edu.cn
作者简介: 赵光灿（1984-），男，浙江绍兴人，硕士生，从事表面等离子共振传感器及检测仪器研究.
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赵光灿,王晓萍,陈惠滨,等. 基于表面等离子共振的全自动浓度检测系统[J]. J4, 2009, 43(8): 1424-1427.

DIAO Guang-Can, WANG Xiao-Ping, CHEN Hui-Bin, et al. Automatic concentration detection system based on surface plasmon resonance. J4, 2009, 43(8): 1424-1427.

链接本文:

http://www.zjujournals.com/eng/CN/10.3785/j.issn.1008-973X.2009.    或    http://www.zjujournals.com/eng/CN/Y2009/V43/I8/1424

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