Please wait a minute...
Journal of Zhejiang University (Agriculture and Life Sciences)  2020, Vol. 46 Issue (1): 83-92    DOI: 10.3785/j.issn.1008-9209.2019.07.111
Research articles     
Effect of 1-methylcyclopropene treatment on storage quality of red Fuji apple
Zhenzhen PENG(),Qihui YE,Xiaoyan XU,Daqi FU()
College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China
Download: HTML   HTML (   PDF(1082KB)
Export: BibTeX | EndNote (RIS)      

Abstract  

In order to study the effects of 1-methylcyclopropene (1-MCP) treatment on the physiological quality and physical changes of fruit during storage time, red Fuji apples were used as research objects. The apple fruit was fumigated with 1.0 μL/L 1-MCP, and then the firmness, soluble solid content, titratable acid content, ethylene release amount, respiratory intensity, anthocyanin content, total phenolic content, and flavonoid content were determined by timing. The results showed that 1-MCP could significantly delay the decrease of firmness and titratable acid content of red Fuji apples. At 24 d, the firmness of red Fuji apples in the treatment group (1.0 μL/L 1-MCP) was 13.54% higher than that in the control group (0 μL/L 1-MCP), and the titratable acid content was 31.78% higher. 1-MCP could delay the increase of soluble solid content, which of the red Fuji apple pulp in the control group was 7.74% higher than that in the treatment group at 24 d of storage. Moreover, the ethylene release amount and respiratory intensity were inhibited (P<0.001). The decrease of the total phenolic content in the peel and pulp and the anthocyanin content in the peel was postponed during the later storage period, as well as the increase of flavonoid content in the peel. In summary, 1-MCP treatment can effectively maintain the storage quality of red Fuji apples after harvesting, which is conducive to short-term storage at the room temperature after harvesting and enhances the economic value of fruits.



Key words1-methylcyclopropene      red Fuji apple      storage      quality     
Received: 11 July 2019      Published: 25 February 2020
CLC:  TS 255.3  
Corresponding Authors: Daqi FU     E-mail: pzzcau@163.com;daqifu@126.com
Cite this article:

Zhenzhen PENG,Qihui YE,Xiaoyan XU,Daqi FU. Effect of 1-methylcyclopropene treatment on storage quality of red Fuji apple. Journal of Zhejiang University (Agriculture and Life Sciences), 2020, 46(1): 83-92.

URL:

http://www.zjujournals.com/agr/10.3785/j.issn.1008-9209.2019.07.111     OR     http://www.zjujournals.com/agr/Y2020/V46/I1/83


1-甲基环丙烯处理对红富士苹果贮藏品质的影响

以红富士苹果为研究对象,利用1.0 μL/L 1-甲基环丙烯(1-methylcyclopropene, 1-MCP)熏蒸苹果果实,通过定时测定果实硬度、可溶性固形物含量、可滴定酸含量、乙烯释放量、呼吸强度、花青素含量、总酚含量、类黄酮含量,研究1-MCP处理对红富士苹果贮藏期生理品质的影响。结果表明:1-MCP可明显延缓红富士苹果硬度和可滴定酸含量下降,在24 d时处理组红富士苹果果肉硬度比对照高13.54%,可滴定酸含量高31.78%;可延缓可溶性固形物含量上升,贮藏24 d时对照组红富士苹果果肉可溶性固形物含量比处理组高7.74%。同样,1-MCP处理可抑制红富士苹果乙烯释放量和呼吸强度(P<0.001),延缓贮藏后期果皮和果肉总酚及果皮花青素含量降低,并延缓贮藏后期果皮类黄酮含量上升。综上所述,1-MCP处理有效保持了红富士苹果采后贮藏品质,有利于采后短期常温贮藏,提升了果实经济价值。


关键词: 1-甲基环丙烯,  红富士苹果,  贮藏,  品质 
Fig. 1 Standard curve of gallic acid
Fig. 2 Standard curve of rutin
Fig. 3 Effect of 1-MCP treatment on the firmness of red Fuji appleCK: Control group (0 μL/L 1-MCP). Double asterisks (**) indicate highly significant differences at the 0.01 probability level.
Fig. 4 Effect of 1-MCP treatment on the titratable acid content of red Fuji appleCK: Control group (0 μL/L 1-MCP). Single asterisk (*) indicates significant differences at the 0.05 probability level; double asterisks (**) indicate highly significant differences at the 0.01 probability level.
Fig. 5 Effect of 1-MCP treatment on the content of soluble solids in red Fuji appleCK: Control group (0 μL/L 1-MCP). Single asterisk (*) indicates significant differences at the 0.05 probability level; double asterisks (**) indicate highly significant differences at the 0.01 probability level.
Fig. 6 Effect of 1-MCP treatment on the release amount of ethylene from red Fuji appleCK: Control group (0 μL/L 1-MCP). Triple asterisks (***) indicate extremely highly significant differences at the 0.001 probability level.
Fig. 7 Effect of 1-MCP treatment on the respiratory intensity of red Fuji appleCK: Control group (0 μL/L 1-MCP). Triple asterisks (***) indicate extremely highly significant differences at the 0.001 probability level.
Fig. 8 Effect of 1-MCP treatment on total phenolic contents in peel (A) and pulp (B) of red Fuji appleCK: Control group (0 μL/L 1-MCP). Single asterisk (*) indicates significant differences at the 0.05 probability level; triple asterisks (***) indicate extremely highly significant differences at the 0.001 probability level.
Fig. 9 Effect of 1-MCP treatment on flavonoid content in peel (A) and pulp (B) of red Fuji appleCK: Control group (0 μL/L 1-MCP). Single asterisk (*) indicates significant differences at the 0.05 probability level; double asterisks (**) indicate highly significant differences at the 0.01 probability level; triple asterisks (***) indicate extremely highly significant differences at the 0.001 probability level.
Fig. 10 Effect of 1-MCP treatment on anthocyanin content of red Fuji appleCK: Control group (0 μL/L 1-MCP). Double asterisks (**) indicate highly significant differences at the 0.01 probability level; triple asterisks (***) indicate extremely highly significant differences at the 0.001 probability level.
[1]   汪景彦.2017年我国各地苹果果价.果树实用技术与信息,2018(2):46.
WANG J Y. Apple fruit prices across China in 2017. Fruit Tree Practical Technology and Information, 2018(2):46. (in Chinese)
[2]   马宝焜.红富士苹果:优质果品生产技术.北京:北京出版社,1993.
MA B K. Red Fuji Apple: High-Quality Fruit Production Technology. Beijing: Beijing Publishing House, 1993. (in Chinese)
[3]   宋卤哲,胡文忠,王雨仝,等.1-MCP处理在果蔬保鲜中的应用进展//中国食品科学技术学会第十五届年会论文摘要集.[2018-11-07].http://cpfd.cnki.com.cn/Area/CPFDCONFArticleList-ZGSP201811001.
SONG L Z, HU W Z, WANG Y T, et al. The application of 1-MCP treatment in the preservation of fruits and vegetables//Abstract Book of the 15th Annual Meeting of the Chinese Institute of Food Science and Technology. [2018-11-07].
[4]   WATKINS C B. 1-Methylcyclopropene (1-MCP) based technologies for storage and shelf life extension. International Journal of Postharvest Technology and Innovation, 2006,1(1):62-68. DOI:10.1504/ijpti.2006.009183
doi: 10.1504/ijpti.2006.009183
[5]   SCHALLER G E, BINDER B M. Inhibitors of ethylene biosynthesis and signaling//Methods in Molecular Biology. New York, US: Humana Press, 2017:223-235.
DOI:10.1007/978-1-4939-6854-1_15
doi: 10.1007/978-1-4939-6854-1_15
[6]   刚成诚,王亦佳,陈奕兆,等.不同1-MCP处理对水蜜桃采后生理及贮藏品质的影响.天津农业科学,2012,18(3):26-32. DOI:10.3969/j.issn.1006-6500.2012.03.006
GANG C C, WANG Y J, CHEN Y Z, et al. Effect of different 1-MCP treatments on water peach postharvest physiology and quality during storage. Tianjin Agricultural Sciences, 2012,18(3):26-32. (in Chinese with English abstract)
doi: 10.3969/j.issn.1006-6500.2012.03.006
[7]   MA Y R, BAN Q F, SHI J Y, et al. 1-Methylcyclopropene (1-MCP), storage time, and shelf life and temperature affect phenolic compounds and antioxidant activity of ‘Jonagold’ apple. Postharvest Biology and Technology, 2019,150:71-79. DOI:10.1016/j.postharvbio.2018.12.015
doi: 10.1016/j.postharvbio.2018.12.015
[8]   曹建康,姜微波,赵玉梅.果蔬采后生理生化实验指导.北京:中国轻工业出版社,2007.
CAO J K, JIANG W B, ZHAO Y M. Guide to Physiology and Biochemistry of Postharvest Fruits and Vegetables. Beijing: China Light Industry Press, 2007. (in Chinese)
[9]   HERNáNDEZ-RUIZ K L, RUIZ-CRUZ S, CIRA-CHAVEZ L A, et al. Evaluation of antioxidant capacity, protective effect on human erythrocytes and phenolic compound identification in two varieties of plum fruit (Spondias spp.) by UPLC-MS. Molecules, 2018,23(12):3200. DOI:10.3390/molecules23123200
doi: 10.3390/molecules23123200
[10]   STOJILJKOVI? D, ARSIC I, TADIC V. Extracts of wild apple fruit (Malus sylvestris (L.) Mill., Rosaceae) as a source of antioxidant substances for use in production of nutraceuticals and cosmeceuticals. Industrial Crop and Products, 2016,80:165-176. DOI:10.1016/j.indcrop.2015.11.023
doi: 10.1016/j.indcrop.2015.11.023
[11]   桑伟娜.贮藏温度对李果实花色苷生物合成的影响.北京:中国农业大学,2018:15-16.
SANG W N. Effects of storage temperature on anthocyanin biosynthesis in plum fruit. Beijing: China Agricultural University, 2018:15-16. (in Chinese with English abstract)
[12]   WATKINS C B. The use of 1-methylcyclopropene (1-MCP) on fruits and vegetables. Biotechnology Advances, 2006,24(4):389-409. DOI:10.1016/j.biotechadv.2006.01.005
doi: 10.1016/j.biotechadv.2006.01.005
[13]   张鹏,陈帅帅,李江阔,等.1-MCP处理对不同成熟度富士苹果贮后货架品质和挥发性物质的影响.食品工业科技,2018,39(21):269-275. DOI:10.13386/j.issn1002-0306.2018.21.047
ZHAGN P, CHEN S S, LI J K, et al. Effect of 1-MCP treatment on quality and volatile substance of apples with different maturity during shelf after cold storage. Science and Technology of Food Industry, 2018,39(21):269-275. (in Chinese with English abstract)
doi: 10.13386/j.issn1002-0306.2018.21.047
[14]   苑克俊,程来亮,牛庆霖,等.苹果果实可溶性固形物含量相关基因的全基因组筛选与分析.植物生理学报,2016,52(5):755-761. DOI:10.13592/j.cnki.ppj.2016.0062
YUAN K J, CHENG L L, NIU Q L, et al. Genome-wide screen and analysis of the genes related to soluble solids content in apple fruits. Journal of Plant Physiology, 2016,52(5):755-761. (in Chinese with English abstract)
doi: 10.13592/j.cnki.ppj.2016.0062
[15]   SISLER E C, SEREK M. Inhibitors of ethylene responses in plants at the receptor level: recent developments. Physiologia Plantarum, 1997,100(3):577-582. DOI:10.1034/j.1399-3054.1997.1000320.x
doi: 10.1034/j.1399-3054
[16]   伍丹,姜睿.浅析基于蝶豆花开发的绿色功能性保健食品.南方农业,2018,12(26):117-118. DOI:10.19415/j.cnki.1673-890x.2018.26.057
WU D, JIANG R. Analysis of green functional health food based on butterfly bean development. South China Agriculture, 2018,12(26):117-118. (in Chinese)
doi: 10.19415/j.cnki.1673-890x.2018.26.057
[17]   陈莉,屠康,赵艺泽,等.采后1-MCP和热处理对红富士苹果生理变化和贮藏品质的影响.果树学报,2006,23(1):59-64. DOI:10.3969/j.issn.1009-9980.2006.01.014
CHEN L, TU K, ZHAO Y Z, et al. Effects of 1-MCP and hot-air treatments on postharvest physiology and quality of red Fuji apples. Journal of Fruit Science, 2006,23(1):59-64. (in Chinese with English abstract)
doi: 10.3969/j.issn.1009-9980.2006.01.014
[18]   韩英群,郭丹,魏鑫,等.果实采后软化生理机制及调控技术研究进展.北方园艺,2017(5):180-183. DOI:10.11937/bfyy.201705042
HAN Y Q, GUO D, WEI X, et al. Research advances on softening physiological mechanisms and its regulation in postharvest fruit. Northern Horticulture, 2017(5):180-183. (in Chinese with English abstract)
doi: 10.11937/bfyy.201705042
[19]   LU X G, MA Y P, LIU X H. Effects of maturity and 1-MCP treatment on postharvest quality and antioxidant properties of ‘Fuji’ apples during long-term cold storage. Horticulture, Environment and Biotechnology, 2012,53(5):378-386. DOI:10.1007/s13580-012-0102-7
doi: 10.1007/s13580-012-0102-7
[20]   颜廷才,秦骅,张鹏,等.1-甲基环丙烯结合ξ-聚赖氨酸对贮后货架期富士苹果的品质及挥发性成分的影响.食品科学,2018,39(9):207-214. DOI:10.7506/spkx1002-6630-201809032
YAN T C, QIN H, ZHANG P, et al. Effects of 1-methylcyclopropene combined with ξ-polylysine on quality and volatile components of Fuji apples during shelf life after cold storage. Food Science, 2018,39(9):207-214. (in Chinese with English abstract)
doi: 10.7506/spkx1002-6630-201809032
[21]   DEELL J R, EHSANI-MOGHADDAM B, BOWEN A J, et al. Effects of 1-methylcyclopropene and controlled atmosphere storage on the quality of ‘Honeycrisp’ apples. Acta Horticulturae, 2015,1071:483-488. DOI:10.17660/actahortic.2015.1071.62
doi: 10.17660/actahortic.2015.1071.62
[22]   LEE J, JEONG M C, KU K H. Chemical, physical, and sensory properties of 1-MCP-treated Fuji apple (Malus domestica Borkh.) fruits after long-term cold storage. Applied Biological Chemistry, 2017,60(4):363-374. DOI:10.1007/s13765-017-0288-6
doi: 10.1007/s13765-017-0288-6
[23]   KOLNIAK-OSTEK J, WOJDVLO A, MARKOWSKI J, et al. 1-Methylcyclopropene postharvest treatment and their effect on apple quality during long-term storage time. European Food Research and Technology, 2014,239(4):603-612. DOI:10.1007/s00217-014-2256-2
doi: 10.1007/s00217-014-2256-2
[24]   王宝春,颉敏华,王学喜,等.1-MCP处理对冷藏期间花牛苹果香气成分和果实品质的影响.食品工业科技,2017,38(7):331-339. DOI:10.13386/j.issn1002-0306.2017.07.056
WANG B C, JIE M H, WANG X X, et al. Effects of 1-MCP treatment on fruit quality and aroma components of Huaniu apples during cold storage. Science and Technology of Food Industry, 2017,38(7):331-339. (in Chinese with English abstract)
doi: 10.13386/j.issn1002-0306.2017.07.056
[25]   PASHAZADEH B, HAJNAJARI H, SHAVAKHI F. 1-MCP improved diverse sensorial, biochemical and physical apple traits during cold storage based on cultivar. Agricultural Sciences, 2017,8(1):77-96. DOI:10.4236/as.2017.81007
doi: 10.4236/as.2017.81007
[26]   LEJA M, MARECZEK A, BEN J. Antioxidant properties of two apple cultivars during long-term storage. Food Chemistry, 2003,80(3):303-307. DOI:10.1016/s0308-8146(02)00263-7
doi: 10.1016/s0308-8146(02)00263-7
[27]   LISTER C E, LANCASTER J E, SUTTON K H, et al. Developmental-changes in the concentration and composition of flavonoids in skin of a red and a green apple cultivar. Journal of the Science of Food and Agriculture, 1994,64(2):155-161. DOI:10.1002/jsfa.2740640204
doi: 10.1002/jsfa.2740640204
[28]   KALINOWSKA M, BIELAWSKA A, LEWANDOWSKA-SIWKIEWICZ H, et al. Apples: content of phenolic compounds vs. variety, part of apple and cultivation model, extraction of phenolic compounds, biological properties. Plant Physiology and Biochemistry, 2014,84:169-188. DOI:10.1016/j.plaphy.2014.09.006
doi: 10.1016/j.plaphy.2014.09.006
[29]   TSAO R, YANG R, YOUNG J C, et al. Polyphenolic profiles in eight apple cultivars using high-performance liquid chroma-tography (HPLC). Journal of Agricultural and Food Chemistry, 2003,51(21):6347-6353. DOI:10.1021/jf0346298
doi: 10.1021/jf0346298
[30]   李秀芳,饶景萍,马秋诗,等.红富士苹果采后1-MCP处理对果皮色素及其相关酶活性的影响.园艺学报,2014,41(3):447-455. DOI:10.16420/j.issn.0513-353x.2014.03.008
LI X F, RAO J P, MA Q S, et al. Effects of 1-MCP treatment on the pericarp pigment and the activity of related enzymes of red Fuji apple. Acta Horticulturae Sinica, 2014,41(3):447-455. (in Chinese with English abstract)
doi: 10.16420/j.issn.0513-353x.2014.03.008
[31]   千春录,林晨,殷健东,等.1-MCP和自发气调对猕猴桃果实贮藏品质和色素的影响.江苏农业科学,2018,46(13):189-192. DOI:10.15889/j.issn.1002-1302.2018.13.045
QIAN C L, LIN C, YIN J D, et al. Impacts of 1-MCP and self-modified atmosphere on quality and pigment of kiwi fruit during storage. Jiangsu Agricultural Sciences, 2018,46(13):189-192. (in Chinese)
doi: 10.15889/j.issn.1002-1302.2018.13.045
[32]   ZHANG M, YUAN B, LENG P. The role of ABA in triggering ethylene biosynthesis and ripening of tomato fruit. Journal of Experimental Botany, 2009,60(6):1579-1588. DOI:10.1093/jxb/erp026
doi: 10.1093/jxb/erp026
[33]   KONDO S, INOUE K. Abscisic acid (ABA) and 1-aminocyclopropane-1-carboxylic acid (ACC) content during growth of ‘Satohnishiki’ cherry fruit, and the effect of ABA and ethephon application on fruit quality. Journal of Horticultural Science and Biotechnology, 1997,72(2):221-227. DOI:10.1080/14620316.1997.11515509
doi: 10.1080/14620316.1997.11515509
[34]   余建,赵爱春,刘长英,等.外源乙烯利与1-MCP处理对桑椹中乙烯和花青素相关代谢基因表达的影响.林业科学,2017,53(2):138-148. DOI:10.11707/j.1001-7488.20170216
YU J, ZHAO A C, LIU C Y, et al. Effects of exogenous ethylene and 1-MCP treatments on the expression of genes involved in ethylene and anthocyanin in mulberry fruit. Scientia Silvae Sinicae, 2017,53(2):138-148. (in Chinese with English abstract)
doi: 10.11707/j.1001-7488.20170216
[35]   刘金.早熟苹果泰山早霞果实着色机理的初步研究.山东,泰安:山东农业大学,2012.
LIU J. Preliminary study of fruit coloration in the precocious apple Taishan Zaoxia. Tai’an, Shandong: Shandong Agricultural University, 2012. (in Chinese with English abstract)
[36]   张艺馨,尚玉臣,张晓丽,等.1-MCP在果蔬应用上的研究进展.中国瓜菜,2016,29(2):1-6. DOI:10.3969/j.issn.1673-2871.2016.11.002
ZHANG Y X, SHANG Y C, ZHANG X L, et al. Research progress on application of 1-MCP in fruits and vegetables. China Cucurbits and Vegetables, 2016,29(2):1-6. (in Chinese with English abstract)
doi: 10.3969/j.issn.1673-2871.2016.11.002
[1] Jue WU,Wenhua XIE,Shuting XU,Yezhi CHEN,Jinping CAO,Yue WANG,Chongde SUN. Effect of high voltage electrostatic field treatment on the storability of postharvest ponkan fruit[J]. Journal of Zhejiang University (Agriculture and Life Sciences), 2020, 46(1): 64-73.
[2] Ling LU,Yida FANG,Li LI,Yanqun XU,Zisheng LUO. Effect of water/oil/water double-preservation emulsion on storage quality of broccoli[J]. Journal of Zhejiang University (Agriculture and Life Sciences), 2020, 46(1): 74-82.
[3] Yan LIU,Xinqi ZHOU,Xiaofeng YU,Yongqiang LI,Shuanglai HAN. Research progress of nondestructive testing techniques for fruit and vegetable quality[J]. Journal of Zhejiang University (Agriculture and Life Sciences), 2020, 46(1): 27-37.
[4] Hanqing ZHANG,Haiyan GAO,Ruiling LIU,Yanchao HAN,Xiangjun FANG,Hangjun CHEN. Effects of packaging of polyethylene bags with different thicknesses on storage quality and lignification of water bamboo[J]. Journal of Zhejiang University (Agriculture and Life Sciences), 2020, 46(1): 55-63.
[5] Qiang ZHOU,Mengjia LIU,Baoshan ZHANG,Changgui LEI,Yuzhu MENG. Combined effect of cinnamon essential oil-chitosan coating and modified atmosphere packaging on the quality of chilled meat[J]. Journal of Zhejiang University (Agriculture and Life Sciences), 2019, 45(6): 723-735.
[6] Qingxia LIN,Lihui XIANG,Lili WANG,Junguo YANG,Zhenshuo SONG,Lin CHEN. Effect of withering temperature on water loss of fresh leaves and quality of white tea[J]. Journal of Zhejiang University (Agriculture and Life Sciences), 2019, 45(4): 434-442.
[7] Chengqiang WANG,Baoshan LI,Jiying WANG,Bingshan HUANG,Tiantian HAO,Yongzhi SUN,Changxing MA,Ying ZHOU. Effects of dietary Bacillus subtilis and yeast culture on growth performance, body composition and farming water quality of juvenile hybrid grouper (Epinephelus fuscoguttatus ×Epinephelus lanceolatus ♂)[J]. Journal of Zhejiang University (Agriculture and Life Sciences), 2019, 45(4): 490-499.
[8] Gang LUO,Jie WANG,Tingting CHEN,Linhai LIU,Songjia LAI. Correlation between leptin gene expression level in different tissues and meat quality in Zika rabbit[J]. Journal of Zhejiang University (Agriculture and Life Sciences), 2019, 45(4): 484-489.
[9] Fangyuan FAN,Xiaolei YANG,Shuying GONG,Haowei GUO,Bangzheng ZONG,Chunlin LI,Hong QIAN,Jianping HU. Study on yellow tea taste characteristic and contribution of taste-chemical compositions to taste quality based on partial main cultivated cultivars in Zhejiang Province[J]. Journal of Zhejiang University (Agriculture and Life Sciences), 2019, 45(4): 443-451.
[10] Fuyin HOU,Yingjiang CHEN,Zhiqing YANG,Chongfu JIN,Kai SHI,Changkuan CHEN,Gongneng FENG,Hongshan LI. Effects of digested pig slurry application on agronomic trait, yield and forage quality of indica rice[J]. Journal of Zhejiang University (Agriculture and Life Sciences), 2019, 45(3): 325-331.
[11] Xinyuan CHEN,Renguo TIAN,Linzhang SHEN,Yiming YIN,Lixin ZHU,Huijuan JIA. Effect of the blue-red light ratio in supplemental light-emitting diode on pitaya flower bud differentiation and fruit quality[J]. Journal of Zhejiang University (Agriculture and Life Sciences), 2019, 45(1): 14-22.
[12] Fuyin HOU,Zhiqing YANG,Changkuan CHEN,Kai SHI,Chongfu JIN,Yingjiang CHEN. Effect of grape seed proanthocyanidin extract on intestine, slaughter performance, lipid metabolism and meat quality of broilers[J]. Journal of Zhejiang University (Agriculture and Life Sciences), 2019, 45(1): 119-125.
[13] XIANG Jie, WANG Fuqiang, GUO Baoguang, WANG Qinggang, YU Chengqun, SHEN Zhenxi, SHAO Xiaoming. Effects of mixtures and intercropping of common vetch and oat in valley area of Tibet on the yield and quality[J]. Journal of Zhejiang University (Agriculture and Life Sciences), 2018, 44(5): 555-564.
[14] NI Jiadan, PAN Shizhe, DAN Fan, JIANG Yihong. Effects of tea polyphenols-chitosan complex solution on changes of quality indicators under storage in Miichthys miiuy[J]. Journal of Zhejiang University (Agriculture and Life Sciences), 2018, 44(5): 594-600.
[15] YANG Hui, KE Leqin, SHU Ruonan, CHEN Yuwei, ZHU Tingyu, WANG Jun, FAN Jintao, WANG Liangliang. Effects of different storage time on polysaccharide content and antioxidant activity of edible fungi[J]. Journal of Zhejiang University (Agriculture and Life Sciences), 2018, 44(3): 356-364.