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Journal of Zhejiang University (Agriculture and Life Sciences)  2019, Vol. 45 Issue (1): 119-125    DOI: 10.3785/j.issn.1008-9209.2017.05.091
Animal sciences & veterinary medicine     
Effect of grape seed proanthocyanidin extract on intestine, slaughter performance, lipid metabolism and meat quality of broilers
Fuyin HOU(),Zhiqing YANG,Changkuan CHEN,Kai SHI,Chongfu JIN,Yingjiang CHEN()
1. Department of Animal Husbandry and Veterinary, Jiangsu Coastal Area Institute of Agricultural Sciences, Yancheng 224002, Jiangsu, China
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Abstract  

In order to explore a green feed additive to reduce the abuse of antibiotics in livestock and poultry farming, grape seed proanthocyanidin extract (GSPE) was selected to study its effects on animal immune protection, growth development and meat quality. In this study, by fermentation bed and cage-free net rearing mode 360 30-day-old broilers were randomized into four groups, with three replicates including 30 broilers each.Broilers in the control group were fed with basal diet, while broilers in the treatment groups were provided with the basal diet supplemented with 100, 200 and 400 mg/kg GSPE, respectively. The experimental period was both 40 d. The results showed as follows: 1) Compared with the control, supplementation with 400 mg/kg GSPE significantly increased the broiler ratio of duodenum villus length/crypt depth (P<0.05), and also induced an increase of goblet cell mucoprotein amount to a certain extent (P>0.05). The supplementation with 100 and 200 mg/kg GSPE promoted the development of broiler intestine without remarkable difference (P>0.05). 2) GSPE improved the slaughter performance of broilers, and the supplementation with 400 mg/kg GSPE possessed the best effect, which significantly increased the percentage of breast muscle than that of the control group (P<0.05). 3) Total serum cholesterol reduced significantly in the treatment when supplemented with 400 mg/kg GSPE (P<0.05) in comparison of the control group. Meanwhile, the supplementation with 100 and 200 mg/kg GSPE decreased triglycerides and low density lipoprotein cholesterol at different levels (P>0.05), but increased the high density lipoprotein cholesterol (P>0.05). 4) The shear force of broiler breast muscle and crude fat of leg muscle were significantly decreased when compared with the control group (P<0.05). Moreover, 200 mg/kg GSPE also largely increased the crude protein of breast muscle (P<0.05). Each experimental group slightly decreased the drip loss rate of muscle (P>0.05). In addition, GSPE did not show any benefit on pH, incarnadine, moisture or collagen content (P>0.05). In conclusion, as a natural plant dietary substance, GSPE could promote the development of broiler intestinal mucosa and improve the slaughter performance, regulate lipid metabolism, and improve meat quality, particularly with 400 mg/kg GSPE, which provides a new insight into practical application.



Key wordsgrape seed proanthocyanidin extract      broiler intestine      slaughter performance      lipid metabolism      meat quality     
Received: 09 May 2017      Published: 28 March 2019
CLC:  S 815  
Corresponding Authors: Yingjiang CHEN     E-mail: houfuyin2008@126.com;cyj-1111@163.com
Cite this article:

Fuyin HOU,Zhiqing YANG,Changkuan CHEN,Kai SHI,Chongfu JIN,Yingjiang CHEN. Effect of grape seed proanthocyanidin extract on intestine, slaughter performance, lipid metabolism and meat quality of broilers. Journal of Zhejiang University (Agriculture and Life Sciences), 2019, 45(1): 119-125.

URL:

http://www.zjujournals.com/agr/10.3785/j.issn.1008-9209.2017.05.091     OR     http://www.zjujournals.com/agr/Y2019/V45/I1/119


原花青素对肉鸡肠道及屠宰性能、脂质代谢和肉品质的影响

为减少畜禽养殖中抗生素等药物的使用量,选取植物源性葡萄籽提取物原花青素(grape seed proanthocyanidin extract, GSPE),探索其对动物免疫保护、生长发育和肉品质等方面的影响。试验采用发酵床网上散养模式,选用360羽30日龄肉鸡,随机分成4组,每组3个重复,每个重复30羽。对照组饲喂基础日粮,试验组分别添加100、200和400 mg/kg GSPE,试验期40 d。结果表明:1)与对照组相比,400 mg/kg GSPE显著增加了肉鸡十二指肠绒毛长度/隐窝深度比值(P<0.05),杯状细胞分泌的黏蛋白数量略有增多(P>0.05),100和200 mg/kg GSPE对肠道形态没有显著影响(P>0.05);2)GSPE提高了肉鸡的屠宰性能,且以添加400 mg/kg GSPE的效果最好,胸肌率较对照组显著提高(P<0.05);3)与对照组相比,400 mg/kg GSPE试验组血清胆固醇含量显著降低(P<0.05),同时,100和200 mg/kg GSPE不同程度地降低了三酰甘油和低密度脂蛋白胆固醇含量(P>0.05),增加了高密度脂蛋白胆固醇含量(P>0.05);4)400 mg/kg GSPE试验组的胸肌剪切力和腿肌粗脂肪含量与对照组相比均显著降低(P<0.05),另外,200 mg/kg GSPE显著提高了胸肌的粗蛋白含量(P<0.05),各剂量组肌肉的滴水损失率稍降低(P>0.05),pH值、肉色、水分和胶原含量没有发生显著变化(P>0.05)。综上所述,作为天然植物饲喂添加剂,GSPE可促进肉鸡的肠道黏膜发育,提高胴体性能,调节脂质代谢,从而进一步改善肉品质,且以400 mg/kg GSPE饲喂的效果最佳,具有较好的开发应用前景。


关键词: 原花青素,  肉鸡肠道,  屠宰性能,  脂质代谢,  肉品质 
Fig. 1 Effect of GSPE on morphological changes of broiler duodenum

处理

Treatment

屠宰率

PDC/%

半净膛率

PHEY/%

全净膛率

PEY/%

胸肌率

PBM/%

腿肌率

PLM/%

T0 91.08±0.50a 81.68±0.37a 69.22±0.57a 22.35±0.72b 20.10±0.61a
T1 90.77±0.89a 83.06±0.83a 70.60±1.30a 24.01±0.97ab 21.25±0.81a
T2 93.13±0.41a 83.83±2.02a 72.51±0.99a 23.74±1.43ab 21.03±1.77a
T3 91.82±1.02a 84.52±0.90a 71.62±1.57a 29.07±3.17a 22.61±1.38a
Table 1 Effect of GSPE on broiler slaughter performance
Fig. 2 Effect of GSPE on broiler lipid metabolism

处理

Treatment

pH

肉色

Incarnadine

剪切力

Shear force/N

滴水损失率

Drip loss rate/%

w(粗蛋白)

Crude protein content/%

w(粗脂肪)

fat content/%

水分

Moisture content/%

w(胶原)

Collagen content/%

T0 5.79±0.04a 76.64±1.50a 3.87±0.43a 36.80±1.15a 23.85±0.10b 2.15±0.04a 74.23±0.08a 1.23±0.05a
T1 5.71±0.20a 80.22±0.93a 3.55±0.28ab 36.73±2.51a 24.11±0.14ab 2.13±0.06a 74.08±0.14a 1.42±0.04a
T2 5.73±0.29a 77.23±1.38a 3.52±0.17ab 35.30±6.68a 25.56±0.74a 2.10±0.05a 74.16±0.07a 1.39±0.02a
T3 6.21±0.38a 77.03±1.84a 2.13±0.28b 33.05±2.29a 24.96±0.76ab 2.03±0.05a 75.77±0.86a 1.39±0.03a
Table 2 Effect of GSPE on meat quality of breast muscle

处理

Treatment

pH

肉色

Incarnadine

剪切力

Shear force/N

滴水损失率

Drip loss rate/%

w(粗蛋白)

Crude protein content/%

w(粗脂肪)

Crude fat content/%

水分

Moisture content/%

w(胶原)

Collagen

content/%

T0 6.31±0.04a 87.22±0.42a 10.06±0.39a 30.39±0.81a 20.83±0.04a 4.27±0.07a 74.45±0.19a 2.52±0.04a
T1 6.20±0.07a 86.98±0.44a 11.66±0.58a 29.17±3.13a 20.36±0.13a 4.11±0.45ab 73.65±0.82a 2.58±0.02a
T2 6.23±0.04a 86.76±0.75a 8.77±0.59a 27.57±2.06a 21.13±0.07a 3.97±0.05ab 73.62±0.06a 2.29±0.03a
T3 6.56±0.40a 87.94±0.86a 8.89±1.46a 24.40±2.37a 22.50±1.11a 3.32±0.32b 74.70±1.01a 2.14±0.05a
 
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