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浙江大学学报（医学版）
浙江大学学报（医学版）  2018, Vol. 47 Issue (1): 10-18    DOI: 10.3785/j.issn.1008-9292.2018.02.02
专题报道     
半胱氨酰白三烯受体对小鼠小胶质细胞吞噬功能的调节作用
王晓蓉1,2(),卢韵碧2,张纬萍2,魏尔清2,方三华2,3,*()
1. 浙江大学医学院附属妇产科医院药剂科, 浙江 杭州 310006
2. 浙江大学医学院药理学系, 浙江 杭州 310058
3. 浙江大学医学院公共技术平台, 浙江 杭州 310058
Effects of cysteinyl leukotriene receptors on phagocytosis of mouse microglial cells
WANG Xiaorong1,2(),LU Yunbi2,ZHANG Weiping2,WEI Erqing2,FANG Sanhua2,3,*()
1. Department of Pharmacy, Women's Hospital, Zhejiang University School of Medicine, Hangzhou 310006, China
2. Department of Pharmacology, Zhejiang University School of Medicine, Hangzhou 310058, China
3. Core Facilities, Zhejiang University School of Medicine, Hangzhou 310058, China
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摘要：

目的: 研究半胱氨酰白三烯（CysLT）受体（CysLT1R和CysLT2R）对小鼠BV2小胶质细胞吞噬功能的调节作用。方法: 以经典炎症激活剂脂多糖和CysLT受体激动剂LTD4处理BV2细胞，采用免疫荧光计数法和流式细胞仪检测BV2细胞吞噬功能，免疫荧光共染法观察BV2细胞中CysLT1R和CysLT2R表达分布。结果: 脂多糖和LTD4均能增强BV2细胞吞噬功能，而CysLT1受体选择性拮抗剂孟鲁司特和CysLT2受体选择性拮抗剂HAMI 3379均能抑制脂多糖和LTD4诱导的BV2细胞吞噬功能增强；脂多糖和LTD4激活BV2细胞后可引起CysLT1R和CysLT2R在细胞内分布变化，两种亚型的受体分布变化趋势基本一致，且存在共表达。结论: CysLT1R和CysLT2R均可以调节BV2细胞的吞噬功能，且两者具有协同性。
关键词： 半胱氨酸/代谢;  受体, 白三烯/代谢;  吞噬作用/药物作用;  小神经胶质细胞/药物作用;  脂多糖类/药理学;  细胞, 培养的    
Abstract:

Objective: To determine the effects of cysteinyl leukotriene receptors (CysLT1R and CysLT2R) on phagocytosis of mouse BV2 microglial cells. Methods: BV2 cells were stimulated with microglial activators lipopolysaccharide (LPS) or CysLT receptor agonists LTD4. The phagocytosis of BV2 cells was observed by immunofluorescence analysis and flow cytometry. The intracellular distributions of CysLT1R and CysLT2R in BV2 cells were examined with immunofluorescence staining. Results: Both LPS and LTD4 could significantly enhance the phagocytosis of BV2 cells, and such effect could be inhibited by CysLT1R selective antagonist Montelukast and CysLT2R selective antagonist HAMI 3379. The activation of BV2 cells induced by LTD4 or LPS resulted in changes in intracellular distributions of CysLT1R and CysLT2R. CysLT1R and CysLT2R was co-localization with a similar distribution. Conclusion: CysLT1R and CysLT2R regulate the phagocytosis of mouse BV2 microglial cells with a synergistic effect.
Key words: Cysteine/metabolism    Receptors, leukotriene/metabolism    Phagocytosis/drug effects    Microglia/drug effects    Lipopolysaccharides/pharmacology    Cells, cultured
收稿日期: 2017-12-07 出版日期: 2018-06-12
CLC:  R743  
基金资助: 浙江省自然科学基金(LY17H090009);浙江省公益技术应用研究（分析测试）计划项目(2016F82G2010036)
通讯作者: 方三华     E-mail: 21118318@zju.edu.cn;fshfbzxhq@zju.edu.cn
作者简介: 王晓蓉(1989-), 女, 硕士, 药师, 主要从事药理学研究; E-mail:21118318@zju.edu.cn; https://orcid.org/0000-0002-2481-0329
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引用本文:

王晓蓉,卢韵碧,张纬萍,魏尔清,方三华. 半胱氨酰白三烯受体对小鼠小胶质细胞吞噬功能的调节作用[J]. 浙江大学学报（医学版）, 2018, 47(1): 10-18.

WANG Xiaorong,LU Yunbi,ZHANG Weiping,WEI Erqing,FANG Sanhua. Effects of cysteinyl leukotriene receptors on phagocytosis of mouse microglial cells. J Zhejiang Univ (Med Sci), 2018, 47(1): 10-18.

链接本文:

http://www.zjujournals.com/med/CN/10.3785/j.issn.1008-9292.2018.02.02    或    http://www.zjujournals.com/med/CN/Y2018/V47/I1/10

图 1  免疫荧光法检测示脂多糖和LTD4诱导BV2细胞吞噬功能增强(n=9)
图 2  孟鲁司特和HAMI 3379对BV2细胞吞噬功能的影响
图 3  免疫荧光法检测示孟鲁司特和HAMI 3379抑制脂多糖或LTD4诱导的BV2细胞吞噬功能增强(n=9)
图 4  孟鲁司特和HAMI 3379抑制脂多糖或LTD4诱导的BV2细胞吞噬功能增强(n=9)
图 5  脂多糖诱导的BV2细胞CysLT受体分布变化
图 6  LTD4诱导的BV2细胞CysLT受体分布变化
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