Effects of Clostridium butyricum on serum biochemical indices, antioxidant function and intestinal microbiota in weaned piglets " /> Clostridium butyricum,weaned piglet,serum biochemical indices,antioxidant function,intestinal microbiota ,"/> 丁酸梭菌对断奶仔猪血清生化指标、抗氧化能力和肠道菌群的影响
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浙江大学学报（农业与生命科学版）
浙江大学学报（农业与生命科学版）  2018, Vol. 44 Issue (2): 190-198    DOI: 10.3785/j.issn.1008-9209.2018.03.016
论文     
丁酸梭菌对断奶仔猪血清生化指标、抗氧化能力和肠道菌群的影响
郑有秀1，王超2，姚静宜1，卢建军1*
1. 浙江大学动物科学学院，浙江省饲料与动物营养重点实验室/农业部（华东）动物营养与饲料重点实验室/浙江大学动物分子营养学教育部重点实验室，杭州 310058；2.南京农业大学动物科技学院，南京 210095

Effects of Clostridium butyricum on serum biochemical indices, antioxidant function and intestinal microbiota in weaned piglets

ZHENG Youxiu1, WANG Chao2, YAO Jingyi1, LU Jianjun1*

1. Key Laboratory of Animal Feed and Nutrition of Zhejiang Province/Key Laboratory of Animal Nutrition and Feed Sciences in East China of Ministry of Agriculture/Key Laboratory of Molecular Animal Nutrition of Ministry of Education, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China; 2. College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China

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摘要：

选取（25±1）日龄体质量相近[（6.24±0.32）kg]的健康“杜×长×大”三元杂交断奶仔猪360 头，随机分为5 组，每组4 个重复，每个重复18 头，研究在饲粮中添加丁酸梭菌对断奶仔猪血清生化指标、抗氧化能力和肠道菌群的影响。对照组饲喂基础饲粮，试验组分别饲喂添加250、500、1 000 和2 000 mg/kg 丁酸梭菌的试验饲粮，试验期30 d。结果显示：1）在饲粮中添加丁酸梭菌对断奶仔猪血清谷草转氨酶、谷丙转氨酶、尿素氮和碱性磷酸酶等生化指标无显著影响（P＞0.05）；与对照组相比，试验组断奶仔猪血清总蛋白水平分别提高了10.45%（P＞0.05）、17.64%（P＜0.05）、15.24%（P＜0.05）和17.54%（P＜0.05），血清白蛋白含量分别提高了0.78%（P＞0.05）、3.80%（P＞0.05）、3.50%（P＞0.05）和5.52%（P＜0.05）。2）添加1 000 mg/kg 丁酸梭菌组的血清过氧化氢酶含量较对照组提高了65.87%（P＜0.05），添加500 mg/kg 丁酸梭菌组的肝总抗氧化能力较对照组提高了13.14%（P＜0.05）。3）在饲粮中添加丁酸梭菌显著或极显著降低了断奶仔猪盲肠大肠埃希菌和沙门菌数量（P＜0.05 或P＜0.01），试验组的乳酸杆菌和双歧杆菌含量均高于对照组，但未达到统计学上的显著水平（P＞0.05）。以上结果表明，在25～55 日龄断奶仔猪饲粮中添加500 mg/kg丁酸梭菌可缓解断奶氧化应激，抑制断奶仔猪肠道有害菌生长。

关键词： 丁酸梭菌;  断奶仔猪;  血清生化指标;  抗氧化能力;  肠道菌群    
Abstract:

To investigate the effects of Clostridium butyricum on serum biochemical indices, antioxidant function and intestinal microbiota in weaned piglets, a total of 360 25-day-old healthy“Duroc×Landrace×Yorkshire” weaned piglets with the average body mass of (6.24±0.32) kg were selected and randomly divided into five groups. Each group had four replicates with 18 piglets per replicate. Piglets in the control group (CG) were fed with a corn-soybean type basal diet, and those in the experimental groups (EG) were fed with experimental diet, which added 250, 500, 1 000 and 2 000 mg/kg Clostridium butyricum in basal diet for 30 days, respectively. The results showed as follows: 1) No significant difference was found between the CG and EG on the serum biochemical indices such as glutamic oxaloacetic transaminase (GOT), glutamate pyruvic transaminase (GPT), blood urea nitrogen (BUN) and alkaline phosphatase (AKP) (P＞0.05). Compared with the CG, the serum total protein (TP) level in the EG was increased by 10.45% (P＞0.05), 17.64% (P＜0.05), 15.24% (P＜0.05) and 17.54% (P＜0.05), respectively; the concentrations of serum albumin (ALB) in the EG were increased by 0.78% (P＞0.05), 3.80% (P＞0.05), 3.50% (P＞0.05) and 5.52% (P＜0.05), respectively. 2) The concentration of serum catalase (CAT) in the 1 000 mg/kg group was increased by 65.87% (P＜0.05). The total antioxidant capacity (TAOC) level of liver in the 500 mg/kg group was increased by 13.14% (P＜0.05). 3) The diet supplemented with Clostridium butyricum significantly decreased the number of Escherichia coli and Salmonella in the cecum of weaned piglets (P＜0.05 or P＜0.01). Compared with the CG, the number of Lactobacillus and Bifidobacterium in the EG increased at different degrees (P＞0.05). The results indicate that the diet supplemented with Clostridium butyricum can alleviate oxidative stress during weaning and inhibit the growth of harmful intestinal bacteria of weaned piglets. The optimal dietary Clostridium butyricum of weaned piglets at the age of 25 to 55 days is 500 mg/kg.

Key words: Clostridium butyricum')" href="#">

Clostridium butyricum    weaned piglet    serum biochemical indices    antioxidant function    intestinal microbiota

出版日期: 2018-04-27
基金资助: 浙江省科技厅重点研发计划项目（2015C02022）；浙江省饲料与动物营养重点实验室开放基金（201705）
通讯作者: 卢建军（https://orcid.org/0000-0003-2060-5827）     E-mail: jjlu@zju.edu.cn
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引用本文:

郑有秀, 王超, 姚静宜, 卢建军. 丁酸梭菌对断奶仔猪血清生化指标、抗氧化能力和肠道菌群的影响[J]. 浙江大学学报（农业与生命科学版）, 2018, 44(2): 190-198.

ZHENG Youxiu, WANG Chao, YAO Jingyi, LU Jianjun.

Effects of Clostridium butyricum on serum biochemical indices, antioxidant function and intestinal microbiota in weaned piglets . Journal of Zhejiang University (Agriculture and Life Sciences), 2018, 44(2): 190-198.

链接本文:

http://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2018.03.016    或    http://www.zjujournals.com/agr/CN/Y2018/V44/I2/190

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