c-Jun 蛋白对A 型流感病毒H1N1 感染小鼠体内CD4＋和 CD8＋ T 细胞增殖的调节作用

doi:10.3785/j.issn.1008-9209.2017.12.132

浙江大学学报（农业与生命科学版）

2018, Vol. 44

Issue (6): 722-726 DOI: 10.3785/j.issn.1008-9209.2017.12.132

动物科学与动物医学

c-Jun 蛋白对A 型流感病毒H1N1 感染小鼠体内CD4＋和 CD8＋ T 细胞增殖的调节作用

张守平^1,2，申汶涛¹，王丽荣¹，张百重^3*

（1.河南科技学院动物科技学院，河南新乡453003；2.中国农业大学动物医学院，北京100192；3.河南科技学院资源与环境学院，河南新乡453003）

c-Jun protein effect on regulating CD^4＋ and CD^8＋ T cell proliferation in H1N1 influenza A virus infected mice

ZHANG Shouping^1,2, SHEN Wentao¹, WANG Lirong¹, ZHANG Baizhong^3*

(1. College of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, Xinxiang 453003, Henan, China; 2. College of Veterinary Medicine, China Agricultural University, Beijing 100192, China; 3. College of Resources and Environment, Henan Institute of Science and Technology, Xinxiang 453003, Henan, China)

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摘要：

通过构建Ａ型流感病毒H1N1 感染小鼠模型，并使用c-Jun 蛋白的特异性核酶Dz13 抑制该蛋白的表达，探索c-Jun 蛋白在流感病毒感染后对T淋巴细胞增殖和炎性反应的调节作用。蛋白质印迹法（Western-blotting）结果表明，核酶Dz13 作用后c-Jun 蛋白的表达量明显降低，且c-Jun 蛋白的活化也随之降低。此外，用流式细胞术测定感染后3 d 和6 d 外周血内辅助性T细胞（CD4^＋T）和杀伤性T细胞（CD8^＋T）的增殖情况，同时用实时荧光定量聚合酶链式反应测定外周血内细胞因子IFN-γ、IL-6 和IL-10 表达。结果显示：小鼠感染病毒后，外周血CD4^＋和CD8^＋T细胞明显增多，相关细胞因子的表达量也明显上调；而抑制c-Jun 蛋白表达后，CD4^＋和CD8^＋T细胞的增殖受到抑制，且相关细胞因子IFN-γ、IL-6 和IL-10 的表达量亦受到抑制。说明c-Jun 蛋白参与调节A型流感病毒H1N1 感染后引起的T淋巴细胞增殖和相关细胞因子的表达。

关键词： c-Jun 蛋白; A型流感病毒; T淋巴细胞; 细胞因子

Abstract: A H1N1 influenza A virus infected mice model was built to evaluate the regulation mechanism of c-Jun protein in T lymphocytes. To determine the DNAzyme Dz13 efficiency in virus-infected animals, a Westernblotting assay was employed to detected total c-Jun (t-c-Jun) and phosphorylation c-Jun (p-c-Jun) levels by specific antibody. It was demonstrated that the drug-treated mice markedly suppressed the expression of t-c-Jun and p-c-Jun. Through flow cytometry and quantitative real-time polymerase chain reaction (qRT-PCR), we could find an obvious CD^4＋ (helper T lymphocytes) and CD^8＋ T (cytotoxic T lymphocyte) cell proliferation and relative cytokine (IFN-γ, IL-6 and IL-10) expression on day 3 and 6 in the virus-infected mice. The Dz13-treated mice with a c-Jun suppression displayed a down-regulation of CD^4＋ and CD^8＋ T cells in peripheral blood. At the same time, Dz13-treated group demonstrated a significant reduction of inflammatory cytokine (IFN-γ, IL-6 and IL-10) expression. In conclusion, c-Jun protein regulates CD^4＋ and CD^8＋ T cell proliferation and inflammatory cytokine expression in H1N1 influenza A virus infected mice.

Key words: c-Jun protein influenza A virus T lymphocyte cytokine

出版日期: 2018-11-25

CLC:

Q 28

基金资助: 中国博士后科学基金面上项目（2016M602243）；河南省科技攻关计划（农业领域）项目（182102110053，182102110039）

通讯作者: 张百重（https://orcid.org/0000-0002-1172-582X） E-mail: 281341651@qq.com

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引用本文:

张守平, 申汶涛, 王丽荣, 张百重. c-Jun 蛋白对A 型流感病毒H1N1 感染小鼠体内CD4＋和 CD8＋ T 细胞增殖的调节作用[J]. 浙江大学学报（农业与生命科学版）, 2018, 44(6): 722-726.

ZHANG Shouping, SHEN Wentao, WANG Lirong, ZHANG Baizhong. c-Jun protein effect on regulating CD^4＋ and CD^8＋ T cell proliferation in H1N1 influenza A virus infected mice. Journal of Zhejiang University (Agriculture and Life Sciences), 2018, 44(6): 722-726.

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http://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2017.12.132 或 http://www.zjujournals.com/agr/CN/Y2018/V44/I6/722

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