| Biomedicine |
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| GM1 stabilizes expression of NMDA receptor subunit 1 in the ischemic hemisphere of MCAo/reperfusion rat |
| LIU Jian-ren, DING Mei-ping, WEI Er-qing, LUO Jian-hong, SONG Ying, HUANG Jian-zheng, GE Qiu-fu, HU Hua, ZHU Li-jun |
| Department of Neurology, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China; Department of Pharmacology, School of Medicine, Zhejiang University, Hangzhou 310009, China; Department of Neurobiology, School of Medicine, Zhejiang University, Hangzhou 310009, China; Department of Neurosurgery, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China |
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Abstract Objective: To determine the protective effect of monosialoganglionside (GM1) and evaluate the influence of GM1 on expression of N-methyl-D-aspartate receptor subunit 1 (NMDAR1) in Sprague-Dawley (SD) rats with focal cerebral ischemia-reperfusion (I/R). Methods: Left middle cerebral artery (MCA) was occluded by an intraluminal suture for 1 h and the brain was reperfused for 72 h in SD rats when infarct volume was measured, GM1 (10 mg/kg) was given ip (intraperitoneally) at 5 min (group A), 1 h (group B) and 2 h (group C) after MCA occlusion (MCAo). Expression of NMDAR1 was detected by Western blot at various time after reperfusion (4 h, 6 h, 24 h, 48 h and 72 h) in ischemic hemispheres of the rats with or without GM1 administered. Results: (1) Adjusted relative infarct volumes of groups A and B were significantly smaller than that of group C and the control group (P<0.01 and P<0.05, respectively). (2) Expression level of NMDAR1 was temporally high at 6 h after reperfusion, and dipped below the normal level at 72 h after reperfusion. GM1 at 5 min after MCAo significantly suppressed the expression of NMDAR1 at 6 h after reperfusion (P<0.05 vs the control). At 72 h after reperfusion, the NMDAR1 expression level of rats treated with GM1 administered (at 5 min or 2 h after MCAo) was significantly higher than that of the control (P<0.05). Conclusion: GM1 can time-dependently reduce infarct volume in rats with focal cerebral I/R partly through stabilizing the expression of NMDAR1.
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Received: 10 May 2004
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Cite this article:
LIU Jian-ren, DING Mei-ping, WEI Er-qing, LUO Jian-hong, SONG Ying, HUANG Jian-zheng, GE Qiu-fu, HU Hua, ZHU Li-jun. GM1 stabilizes expression of NMDA receptor subunit 1 in the ischemic hemisphere of MCAo/reperfusion rat. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2005, 6(4): 254-258.
URL:
http://www.zjujournals.com/xueshu/zjus-b/10.1631/jzus.2005.B0254 OR http://www.zjujournals.com/xueshu/zjus-b/Y2005/V6/I4/254
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