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ZJUS-B
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology)  2010, Vol. 11 Issue (4): 267-274    DOI: 10.1631/jzus.B0900390
Biomedicine     
Effects of sevoflurane preconditioning and postconditioning on rat myocardial stunning in ischemic reperfusion injury
An-lu Dai, Li-hua Fan, Feng-jiang Zhang, Mei-juan Yang, Jing Yu, Jun-kuan Wang, Tao Fang, Gang Chen, Li-na Yu, Min Yan
Department of Anesthesiology, the Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China, Department of Anesthesiology, Lishui People\'s Hospital, Lishui 323600, China, Department of Anesthesiology, Jinhua People\'s Hospital, Jinhua 321300, China
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Abstract  Ischemic preconditioning and postconditioning distinctly attenuate ventricular arrhythmia after ischemia without affecting the severity of myocardial stunning. Therefore, we report the effects of sevoflurane preconditioning and postconditioning on stunned myocardium in isolated rat hearts. Isolated rat hearts were underwent 20 min of global ischemia and 40 min of reperfusion. After an equilibration period (20 min), the hearts in the preconditioning group were exposed to sevoflurane for 5 min and next washout for 5 min before ischemia. Hearts in the sevoflurane postconditioning group underwent equilibration and ischemia, followed immediately by sevoflurane exposure for the first 5 min of reperfusion. The control group received no treatment before and after ischemia. Left ventricular pressure, heart rate, coronary flow, electrocardiogram, and tissue histology were measured as variables of ventricular function and cellular injury, respectively. There was no significant difference in the duration of reperfusion ventricular arrhythmias between control and sevoflurane preconditioning group (P=0.195). The duration of reperfusion ventricular arrhythmias in the sevoflurane postconditioning group was significantly shorter than that in the other two groups (P<0.05). ±(dP/dt)max in the sevoflurane preconditioning group at 5, 10, 15, 20, and 30 min after reperfusion was significantly higher than that in the control group (P<0.05), and there were no significant differences at 40 min after reperfusion among the three groups (P>0.05). As expected, for a 20-min general ischemia, infarct size in heart slices determined by 2,3,5-triphenyltetrazolium chloride staining among the groups was not obvious. Sevoflurane postconditioning reduces reperfusion arrhythmias without affecting the severity of myocardial stunning. In contrast, sevoflurane preconditioning has no beneficial effects on reperfusion arrhythmias, but it is in favor of improving ventricular function and recovering myocardial stunning. Sevoflurane preconditioning and postconditioning may be useful for correcting the stunned myocardium.

Key words： Inhalation anesthetics      Sevoflurane      Postconditioning      Preconditioning      Ischemia-reperfusion injury      Myocardial stunning     
Received: 20 December 2009      Published: 29 March 2010
CLC:  R614  
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Li-na Yu
Min Yan
An-lu Dai
Li-hua Fan
Feng-jiang Zhang
Mei-juan Yang
Jing Yu
Jun-kuan Wang
Tao Fang
Gang Chen
Cite this article:

An-lu Dai, Li-hua Fan, Feng-jiang Zhang, Mei-juan Yang, Jing Yu, Jun-kuan Wang, Tao Fang, Gang Chen, Li-na Yu, Min Yan. Effects of sevoflurane preconditioning and postconditioning on rat myocardial stunning in ischemic reperfusion injury. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2010, 11(4): 267-274.

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http://www.zjujournals.com/xueshu/zjus-b/10.1631/jzus.B0900390     OR     http://www.zjujournals.com/xueshu/zjus-b/Y2010/V11/I4/267

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