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ZJUS-B
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology)  2014, Vol. 15 Issue (8): 735-742    DOI: 10.1631/jzus.B1300196
Articles     
Protective effect of indomethacin in renal ischemia-reperfusion injury in mice
Sheng-hong Zhu, Li-jia Zhou, Hong Jiang, Rong-jun Chen, Chuan Lin, Shi Feng, Juan Jin, Jiang-hua Chen, Jian-yong Wu
Kidney Disease Center, the First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China; Kidney Disease Center, Shaoxing Second Hospital, Shaoxing 312000, China
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Abstract  Objective: To evaluate the renoprotection effects of non-steroidal anti-inflammatory drugs (NSAIDs) in renal ischemia-reperfusion injury (IRI) and the cyclooxygenase (COX)-1/2 blockade association by indomethacin (IMT) in the mice model. Methods: After the left renal pedicle of mice was clamped, IMT was administrated by intraperitoneal injection with four doses: 1, 3, 5, and 7 mg/kg. Blood and kidney samples were collected 24 h after IRI. The renal functions were assayed by the cytokines and serum creatinine (SCr) using enzyme-linked immunosorbent assay (ELISA) kits. Kidney samples were analyzed by hematoxylin and eosin (H&E) and immunohistochemistry stainings. Results: The mice administered with 5 mg/kg IMT had a marked reduction in SCr and significantly less tubular damage. The tumor necrosis factor α (TNF-α) activity in renal homogenates and interleukin 6 (IL-6) activity in serum had a marked reduction at doses of 5 and 7 mg/kg IMT. The administration of 3 and 5 mg/kg IMT had a marked reduction in the ratio of thromboxane B2 to 6-keto-prostaglandin F1α. COX-1 and COX-2 stainings were weaker in 5 mg/kg IMT groups than that in the other groups. Conclusions: There was a dose response in the IMT function of renal IRI in mice, and IMT had a protective effect in a certain dose range. The effect of IMT on mice IRI was related to COX-1/2 blockades.

Key words： Non-steroidal anti-inflammatory drug (NSAID)      Indomethacin (IMT)      Ischemia-reperfusion injury (IRI)      Dosage      Protective effect     
Received: 24 July 2013      Published: 05 August 2014
CLC:  R58  
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Jian-yong Wu
Sheng-hong Zhu
Li-jia Zhou
Hong Jiang
Rong-jun Chen
Chuan Lin
Shi Feng
Juan Jin
Jiang-hua Chen
Cite this article:

Sheng-hong Zhu, Li-jia Zhou, Hong Jiang, Rong-jun Chen, Chuan Lin, Shi Feng, Juan Jin, Jiang-hua Chen, Jian-yong Wu. Protective effect of indomethacin in renal ischemia-reperfusion injury in mice. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2014, 15(8): 735-742.

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http://www.zjujournals.com/xueshu/zjus-b/10.1631/jzus.B1300196     OR     http://www.zjujournals.com/xueshu/zjus-b/Y2014/V15/I8/735

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