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浙江大学学报(工学版)
J4  2012, Vol. 46 Issue (2): 345-350    DOI: 10.3785/j.issn.1008-973X.2012.02.026
    
The application of SAM technology in biocompatibility
design of cell-based biosensor
ZHANG Wei, HU Liang, XIAO Li-dan, HU Zhao-ying, WANG Ping
Biosensor National Special Laboratory, Key Laboratory of Biomedical Engineering of Ministry of Education,
Department ofBiomedical Engineering Zhejiang University, Hangzhou 310027, China
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Abstract  

To improve the biocompatibility of cell-based biosensor and immobilize cells in target position, self-assembled monolayer (SAM) technology was applied to modify the surface of cell-based biosensor. It utilized the Van der Waals attraction among different functional groups in organic molecular to assemble a thin film layer-by-layer in nano-scale, which made electrode region attracting cells, while background expelling cells. In this manner, cells were guided to adhere onto electrode region efficiently. Fluorescent imaging, electrochemical test and real-time impedance scanning were employed to evaluate the modified surface. The results demonstrate it behaved excellent biocompatibility and stable electrochemical properties, which is suitable for cell viability. Meanwhile, it can promote cells to adhere on target position successfully. Sequentially, we manage to culture cardiac myocytes from newborn rat on SAM modified MEA chip. And extracellular electrophysiological measurement is also carried out. It exhibits a promising prospect in further application of cell electrophysiological field.

Published: 20 March 2012
CLC:  TP 212.3  
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Cite this article:

ZHANG Wei, HU Liang, XIAO Li-dan, HU Zhao-ying, WANG Ping. The application of SAM technology in biocompatibility
design of cell-based biosensor. J4, 2012, 46(2): 345-350.

URL:

http://www.zjujournals.com/eng/10.3785/j.issn.1008-973X.2012.02.026     OR     http://www.zjujournals.com/eng/Y2012/V46/I2/345


自组装技术在细胞传感器相容性设计中的应用

为了提高细胞传感器表面的生物相容性并引导细胞定向定位生长,采用自组装单分子层（SAM）技术修饰细胞传感器表面,利用有机分子各官能团之间相互作用的范德华力层层组装成纳米尺寸薄膜,使电极测试区域吸引细胞,而背景区域排斥细胞,有效地引导细胞贴附在电极区域上.并使用荧光标记,电化学扫描和实时阻抗扫描方法对SAM修饰后表面评估.结果表明，该表面适合细胞生存生长,具有良好的生物相容性和稳定的电化学特性,能够大大提高细胞定位的成功率.在SAM技术修饰的微电极阵列传感器（MEA）表面培养乳鼠心肌细胞并进行胞外电位检测实验,进一步表明SAM技术可被应用于细胞电生理研究领域.

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