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| 反应条件对DBBF修饰猪血红蛋白性能的影响 |
| CAI Jin1, MENG Wen-fang2, YUAN Zhong-yi3, CEN Pei-lin1 |
(1. Department of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, China;
2. School of Medicine, Zhejiang University, Hangzhou 310016 China;
3.Institute of Biochemistry and Cell Biology, Shanghai Institutes of Life Science, The Chinese Academy of Sciences, Shanghai 200031, China) |
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Abstract Effect of different reaction conditions on properties of DBBF-modified porcine hemoglobin products
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Published: 16 January 2010
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反应条件对DBBF修饰猪血红蛋白性能的影响
猪血红蛋白在脱氧条件下经双（3,5-二溴水杨酸）延胡索酸酯(DBBF)修饰,其携氧能力得到改善,氧合曲线较未经修饰血红蛋白右移,半饱和氧分压（P50值）为3.1 kPa,希尔系数值为2.2,两者都接近人全血值；修饰过程中别构效应调节剂对修饰产物携氧性能影响很大,在反应混合物中加入5.0 mmol/L六磷酸肌醇,产物的P50明显增加.DBBF与血红蛋白的量比为2∶1,反应时间2 h时,产物的P50和希尔系数均接近人体生理指标.电泳图谱分析表明,无基质猪血红蛋白可完全解聚为单体,只在相对分子质量为15×103处出现条带,而DBBF修饰产物分别在相对分子质量为15×103和31×103处同现条带，提示DBBF对猪血红蛋白的两个亚基进行了分子内共价交联.
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